Effects of human OEC-derived cell transplants in rodent spinal cord contusion injury

Gorrie, CA; Hayward, I; Cameron, N; Kailainathan, G; Nandapalan, N; Sutharsan, R; Wang, J; Mackay-Sim, A; Waite, PME

Effects of human OEC-derived cell transplants in rodent spinal cord contusion injury

Gorrie, CA

Hayward, I Cameron, N Kailainathan, G Nandapalan, N Sutharsan, R Wang, J Mackay-Sim, A Waite, PME

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Publication Type:: Journal Article
Citation:: Brain Research, 2010, 1337 pp. 8 - 20
Issue Date:: 2010-06-14

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Field	Value	Language
dc.contributor.author	Gorrie, CA https://orcid.org/0000-0001-5934-2492	en_US
dc.contributor.author	Hayward, I	en_US
dc.contributor.author	Cameron, N	en_US
dc.contributor.author	Kailainathan, G	en_US
dc.contributor.author	Nandapalan, N	en_US
dc.contributor.author	Sutharsan, R	en_US
dc.contributor.author	Wang, J	en_US
dc.contributor.author	Mackay-Sim, A	en_US
dc.contributor.author	Waite, PME	en_US
dc.date.available	2010-04-09	en_US
dc.date.issued	2010-06-14	en_US
dc.identifier.citation	Brain Research, 2010, 1337 pp. 8 - 20	en_US
dc.identifier.issn	0006-8993	en_US
dc.identifier.uri	http://hdl.handle.net/10453/15945
dc.description.abstract	Numerous reports indicate that rodent olfactory ensheathing cells (OECs) assist in spinal cord repair and clinical trials have been undertaken using autologous transplantation of human olfactory ensheathing cells (hOECs) as a treatment for spinal cord injury. However, there are few studies investigating the efficacy of hOECs in animal models of spinal cord injury. In this study hOECs were derived from biopsies of human olfactory mucosa, purifed by culture in a serum-free medium containing neurotrophin-3, genetically labelled with EGFP, and stored frozen. These hOEC-derived cells were thawed and transplanted into the spinal cord injury site 7 days after a moderate contusion injury of the spinal cord at thoracic level T10 in the athymic rat. Six weeks later the animals receiving the hOEC-derived transplants had greater functional improvement in their hindlimbs than controls, assessed using open field (BBB scale) and horizontal rung walking tests. Histological analysis demonstrated beneficial effects of hOEC-derived cell transplantation: reductions in the volume of the lesion and the cavities within the lesion. The transplanted cells were located at the periphery of the lesion where they integrated with GFAP-positive astrocytes resulting in a significant reduction of GFAP staining intensity adjacent to the lesion. Although their mechanism of action is unclear we conclude that hOEC-derived cell transplants improved functional recovery after transplantation into the contused spinal cord, probably by modulating inflammatory responses and reducing secondary damage to the cord. © 2010 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Brain Research	en_US
dc.relation.isbasedon	10.1016/j.brainres.2010.04.019	en_US
dc.subject.classification	Neurology & Neurosurgery	en_US
dc.subject.mesh	Olfactory Mucosa	en_US
dc.subject.mesh	Astrocytes	en_US
dc.subject.mesh	Cells, Cultured	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Rats	en_US
dc.subject.mesh	Rats, Nude	en_US
dc.subject.mesh	Spinal Cord Injuries	en_US
dc.subject.mesh	Neurotrophin 3	en_US
dc.subject.mesh	Green Fluorescent Proteins	en_US
dc.subject.mesh	Culture Media, Serum-Free	en_US
dc.subject.mesh	Fluorescent Dyes	en_US
dc.subject.mesh	Cryopreservation	en_US
dc.subject.mesh	Cell Transplantation	en_US
dc.subject.mesh	Models, Animal	en_US
dc.subject.mesh	Recovery of Function	en_US
dc.subject.mesh	Female	en_US
dc.title	Effects of human OEC-derived cell transplants in rodent spinal cord contusion injury	en_US
dc.type	Journal Article
utslib.citation.volume	1337	en_US
utslib.for	1109 Neurosciences	en_US
utslib.for	1701 Psychology	en_US
utslib.for	1702 Cognitive Sciences	en_US
dc.location.activity	ISI:000279207400002	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	1337	en_US

Abstract:

Numerous reports indicate that rodent olfactory ensheathing cells (OECs) assist in spinal cord repair and clinical trials have been undertaken using autologous transplantation of human olfactory ensheathing cells (hOECs) as a treatment for spinal cord injury. However, there are few studies investigating the efficacy of hOECs in animal models of spinal cord injury. In this study hOECs were derived from biopsies of human olfactory mucosa, purifed by culture in a serum-free medium containing neurotrophin-3, genetically labelled with EGFP, and stored frozen. These hOEC-derived cells were thawed and transplanted into the spinal cord injury site 7 days after a moderate contusion injury of the spinal cord at thoracic level T10 in the athymic rat. Six weeks later the animals receiving the hOEC-derived transplants had greater functional improvement in their hindlimbs than controls, assessed using open field (BBB scale) and horizontal rung walking tests. Histological analysis demonstrated beneficial effects of hOEC-derived cell transplantation: reductions in the volume of the lesion and the cavities within the lesion. The transplanted cells were located at the periphery of the lesion where they integrated with GFAP-positive astrocytes resulting in a significant reduction of GFAP staining intensity adjacent to the lesion. Although their mechanism of action is unclear we conclude that hOEC-derived cell transplants improved functional recovery after transplantation into the contused spinal cord, probably by modulating inflammatory responses and reducing secondary damage to the cord. © 2010 Elsevier B.V. All rights reserved.

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http://hdl.handle.net/10453/15945