A Cyanobacteria Enriched Layer of Shark Bay Stromatolites Reveals a New Acaryochloris Strain Living in Near Infrared Light.

Johnson, MS; Burns, BP; Herdean, A; Angeloski, A; Ralph, P; Morris, T; Kindler, G; Wong, HL; Kuzhiumparambil, U; Sedger, LM; Larkum, AWD

A Cyanobacteria Enriched Layer of Shark Bay Stromatolites Reveals a New Acaryochloris Strain Living in Near Infrared Light.

Johnson, MS Burns, BP Herdean, A

Angeloski, A

Ralph, P Morris, T Kindler, G Wong, HL Kuzhiumparambil, U Sedger, LM Larkum, AWD

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Microorganisms, 2022, 10, (5), pp. 1035
Issue Date:: 2022-05-17

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Field	Value	Language
dc.contributor.author	Johnson, MS
dc.contributor.author	Burns, BP
dc.contributor.author	Herdean, A https://orcid.org/0000-0003-2143-0213
dc.contributor.author	Angeloski, A https://orcid.org/0000-0003-4395-1829
dc.contributor.author	Ralph, P
dc.contributor.author	Morris, T
dc.contributor.author	Kindler, G
dc.contributor.author	Wong, HL
dc.contributor.author	Kuzhiumparambil, U
dc.contributor.author	Sedger, LM
dc.contributor.author	Larkum, AWD
dc.date.accessioned	2022-08-31T05:15:48Z
dc.date.available	2022-05-05
dc.date.available	2022-08-31T05:15:48Z
dc.date.issued	2022-05-17
dc.identifier.citation	Microorganisms, 2022, 10, (5), pp. 1035
dc.identifier.issn	2076-2607
dc.identifier.issn	2076-2607
dc.identifier.uri	http://hdl.handle.net/10453/161136
dc.description.abstract	The genus Acaryochloris is unique among phototrophic organisms due to the dominance of chlorophyll d in its photosynthetic reaction centres and light-harvesting proteins. This allows Acaryochloris to capture light energy for photosynthesis over an extended spectrum of up to ~760 nm in the near infra-red (NIR) spectrum. Acaryochloris sp. has been reported in a variety of ecological niches, ranging from polar to tropical shallow aquatic sites. Here, we report a new Acarychloris strain isolated from an NIR-enriched stratified microbial layer 4-6 mm under the surface of stromatolite mats located in the Hamelin Pool of Shark Bay, Western Australia. Pigment analysis by spectrometry/fluorometry, flow cytometry and spectral confocal microscopy identifies unique patterns in pigment content that likely reflect niche adaption. For example, unlike the original A. marina species (type strain MBIC11017), this new strain, Acarychloris LARK001, shows little change in the chlorophyll d/a ratio in response to changes in light wavelength, displays a different Fv/Fm response and lacks detectable levels of phycocyanin. Indeed, 16S rRNA analysis supports the identity of the A. marina LARK001 strain as close to but distinct from from the A. marina HICR111A strain first isolated from Heron Island and previously found on the Great Barrier Reef under coral rubble on the reef flat. Taken together, A. marina LARK001 is a new cyanobacterial strain adapted to the stromatolite mats in Shark Bay.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation.ispartof	Microorganisms
dc.relation.isbasedon	10.3390/microorganisms10051035
dc.rights	info:eu-repo/semantics/openAccess
dc.title	A Cyanobacteria Enriched Layer of Shark Bay Stromatolites Reveals a New Acaryochloris Strain Living in Near Infrared Light.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	Switzerland
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2022-08-31T05:15:33Z
pubs.issue	5
pubs.publication-status	Published online
pubs.volume	10
utslib.citation.issue	5

Abstract:

The genus Acaryochloris is unique among phototrophic organisms due to the dominance of chlorophyll d in its photosynthetic reaction centres and light-harvesting proteins. This allows Acaryochloris to capture light energy for photosynthesis over an extended spectrum of up to ~760 nm in the near infra-red (NIR) spectrum. Acaryochloris sp. has been reported in a variety of ecological niches, ranging from polar to tropical shallow aquatic sites. Here, we report a new Acarychloris strain isolated from an NIR-enriched stratified microbial layer 4-6 mm under the surface of stromatolite mats located in the Hamelin Pool of Shark Bay, Western Australia. Pigment analysis by spectrometry/fluorometry, flow cytometry and spectral confocal microscopy identifies unique patterns in pigment content that likely reflect niche adaption. For example, unlike the original A. marina species (type strain MBIC11017), this new strain, Acarychloris LARK001, shows little change in the chlorophyll d/a ratio in response to changes in light wavelength, displays a different Fv/Fm response and lacks detectable levels of phycocyanin. Indeed, 16S rRNA analysis supports the identity of the A. marina LARK001 strain as close to but distinct from from the A. marina HICR111A strain first isolated from Heron Island and previously found on the Great Barrier Reef under coral rubble on the reef flat. Taken together, A. marina LARK001 is a new cyanobacterial strain adapted to the stromatolite mats in Shark Bay.

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http://hdl.handle.net/10453/161136