Enzymatic hydrolysis using ultrasound for bioethanol production from durian (durio zibethinus) seeds as potential bio fuel

Sebayang, AH; Hasan, MH; Chyuan, OH; Dharma, S; Bahar, AH; Silitonga, AS; Kusumo, F

Enzymatic hydrolysis using ultrasound for bioethanol production from durian (durio zibethinus) seeds as potential bio fuel

Sebayang, AH Hasan, MH Chyuan, OH Dharma, S Bahar, AH Silitonga, AS Kusumo, F

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Publisher:: Italian Association of Chemical Engineering - AIDIC
Publication Type:: Journal Article
Citation:: Chemical Engineering Transactions, 2017, 56, pp. 553-558
Issue Date:: 2017-01-01

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Field	Value	Language
dc.contributor.author	Sebayang, AH
dc.contributor.author	Hasan, MH
dc.contributor.author	Chyuan, OH
dc.contributor.author	Dharma, S
dc.contributor.author	Bahar, AH
dc.contributor.author	Silitonga, AS
dc.contributor.author	Kusumo, F
dc.date.accessioned	2022-09-08T01:41:00Z
dc.date.available	2022-09-08T01:41:00Z
dc.date.issued	2017-01-01
dc.identifier.citation	Chemical Engineering Transactions, 2017, 56, pp. 553-558
dc.identifier.isbn	9788895608471
dc.identifier.issn	2283-9216
dc.identifier.issn	2283-9216
dc.identifier.uri	http://hdl.handle.net/10453/161514
dc.description.abstract	The appealing second generation bioethanol production brings a good promise to achieve a fuel production that is renewable and sustainable; this makes durian (Durio zibethinus) seed interesting to take advantage of, especially for a tropical country like Malaysia. This paper aims to produce bioethanol from durian seed by utilizing ultrasound technique in its enzymatic hydrolysis process. 9 % (w/v) pre-Treated durian seed was brought into the ultrasound-Assisted glass reactor to begin the liquefaction and saccharification processes. Bacillus licheniformis Type XII-A was employed, and ultrasound at 50% amplitude for 60 min was set for liquefaction process; while amyloglucosidase from Aspergillus niger was used, and ultrasound at 40% amplitude for 120 min was run for saccharification process. The sum of both processes in hydrolysis yielded 41.07 g/L of reducing sugar, which was immediately brought to fermentation stage. Saccharomyces cerevisiae was employed for fermentation and resulted 18.48 g/L (0.44 g ethanol/g glucose), which is equivalent to 86.27 % of theoretical ethanol yield (0.51 g ethanol/g glucose) after 84 h of fermentation at 37 °C with 150 rpm incubator shaker. The ethanol purity was improved in the next stage, distillation. Using zeolite as adsorbent, ethanol with purity of 95.7% (v/v) was produced. From the acquired results, durian seed shows a justifiably potential as a second generation bioethanol feedstock. To further improve its potential, studies of optimization using this feedstock is highly encouraged.
dc.language	en
dc.publisher	Italian Association of Chemical Engineering - AIDIC
dc.relation.ispartof	Chemical Engineering Transactions
dc.relation.isbasedon	10.3303/CET1756093
dc.rights	info:eu-repo/semantics/closedAccess
dc.title	Enzymatic hydrolysis using ultrasound for bioethanol production from durian (durio zibethinus) seeds as potential bio fuel
dc.type	Journal Article
utslib.citation.volume	56
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2022-09-08T01:40:58Z
pubs.publication-status	Published
pubs.volume	56

Abstract:

The appealing second generation bioethanol production brings a good promise to achieve a fuel production that is renewable and sustainable; this makes durian (Durio zibethinus) seed interesting to take advantage of, especially for a tropical country like Malaysia. This paper aims to produce bioethanol from durian seed by utilizing ultrasound technique in its enzymatic hydrolysis process. 9 % (w/v) pre-Treated durian seed was brought into the ultrasound-Assisted glass reactor to begin the liquefaction and saccharification processes. Bacillus licheniformis Type XII-A was employed, and ultrasound at 50% amplitude for 60 min was set for liquefaction process; while amyloglucosidase from Aspergillus niger was used, and ultrasound at 40% amplitude for 120 min was run for saccharification process. The sum of both processes in hydrolysis yielded 41.07 g/L of reducing sugar, which was immediately brought to fermentation stage. Saccharomyces cerevisiae was employed for fermentation and resulted 18.48 g/L (0.44 g ethanol/g glucose), which is equivalent to 86.27 % of theoretical ethanol yield (0.51 g ethanol/g glucose) after 84 h of fermentation at 37 °C with 150 rpm incubator shaker. The ethanol purity was improved in the next stage, distillation. Using zeolite as adsorbent, ethanol with purity of 95.7% (v/v) was produced. From the acquired results, durian seed shows a justifiably potential as a second generation bioethanol feedstock. To further improve its potential, studies of optimization using this feedstock is highly encouraged.

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http://hdl.handle.net/10453/161514