Green Tea Polyphenols Modify the Gut Microbiome in db/db Mice as Co-Abundance Groups Correlating with the Blood Glucose Lowering Effect.

Chen, T; Liu, AB; Sun, S; Ajami, NJ; Ross, MC; Wang, H; Zhang, L; Reuhl, K; Kobayashi, K; Onishi, JC; Zhao, L; Yang, CS

Green Tea Polyphenols Modify the Gut Microbiome in db/db Mice as Co-Abundance Groups Correlating with the Blood Glucose Lowering Effect.

Chen, T Liu, AB Sun, S Ajami, NJ Ross, MC Wang, H Zhang, L

Reuhl, K Kobayashi, K Onishi, JC Zhao, L Yang, CS

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Mol Nutr Food Res, 2019, 63, (8), pp. e1801064
Issue Date:: 2019-04

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Field	Value	Language
dc.contributor.author	Chen, T
dc.contributor.author	Liu, AB
dc.contributor.author	Sun, S
dc.contributor.author	Ajami, NJ
dc.contributor.author	Ross, MC
dc.contributor.author	Wang, H
dc.contributor.author	Zhang, L https://orcid.org/0000-0003-0641-2383
dc.contributor.author	Reuhl, K
dc.contributor.author	Kobayashi, K
dc.contributor.author	Onishi, JC
dc.contributor.author	Zhao, L
dc.contributor.author	Yang, CS
dc.date.accessioned	2022-09-14T02:02:02Z
dc.date.available	2022-09-14T02:02:02Z
dc.date.issued	2019-04
dc.identifier.citation	Mol Nutr Food Res, 2019, 63, (8), pp. e1801064
dc.identifier.issn	1613-4125
dc.identifier.issn	1613-4133
dc.identifier.uri	http://hdl.handle.net/10453/161813
dc.description.abstract	SCOPE: The effects of green tea polyphenols, Polyphenon E (PPE), and black tea polyphenols, theaflavins (TFs), on gut microbiota and development of diabetes in db/db mice are investigated and compared. METHODS AND RESULTS: Supplementation of PPE (0.1%) in the diet of female db/db mice for 7 weeks decreases fasting blood glucose levels and mesenteric fat while increasing the serum level of insulin, possibly through protection against β-cell damage. However, TFs are less or not effective. Microbiome analysis through 16S rRNA gene sequencing shows that PPE and TFs treatments significantly alter the bacterial community structure in the cecum and colon, but not in the ileum. The key bacterial phylotypes responding to the treatments are then clustered into 11 co-abundance groups (CAGs). CAGs 6 and 7, significantly increased by PPE but not by TFs, are negatively associated with blood glucose levels. The operational taxonomic units in these CAGs are from two different phyla, Firmicutes and Bacteroidetes. CAG 10, decreased by PPE and TFs, is positively associated with blood glucose levels. CONCLUSION: Gut microbiota respond to tea polyphenol treatments as CAGs instead of taxa. Some of the CAGs associated with the blood glucose lowering effect are enriched by PPE, but not TFs.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation.ispartof	Mol Nutr Food Res
dc.relation.isbasedon	10.1002/mnfr.201801064
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0908 Food Sciences, 1111 Nutrition and Dietetics, 1117 Public Health and Health Services
dc.subject.classification	Food Science
dc.subject.classification	Nutrition & Dietetics
dc.subject.mesh	Animals
dc.subject.mesh	Biflavonoids
dc.subject.mesh	Blood Glucose
dc.subject.mesh	Body Weight
dc.subject.mesh	Catechin
dc.subject.mesh	Female
dc.subject.mesh	Gastrointestinal Microbiome
dc.subject.mesh	Hypoglycemic Agents
dc.subject.mesh	Insulin
dc.subject.mesh	Insulin-Secreting Cells
dc.subject.mesh	Mice, Mutant Strains
dc.subject.mesh	Organ Size
dc.subject.mesh	Polyphenols
dc.subject.mesh	RNA, Ribosomal, 16S
dc.subject.mesh	Tea
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Mutant Strains
dc.subject.mesh	Body Weight
dc.subject.mesh	Catechin
dc.subject.mesh	Biflavonoids
dc.subject.mesh	Insulin
dc.subject.mesh	Blood Glucose
dc.subject.mesh	RNA, Ribosomal, 16S
dc.subject.mesh	Hypoglycemic Agents
dc.subject.mesh	Organ Size
dc.subject.mesh	Tea
dc.subject.mesh	Female
dc.subject.mesh	Insulin-Secreting Cells
dc.subject.mesh	Polyphenols
dc.subject.mesh	Gastrointestinal Microbiome
dc.title	Green Tea Polyphenols Modify the Gut Microbiome in db/db Mice as Co-Abundance Groups Correlating with the Blood Glucose Lowering Effect.
dc.type	Journal Article
utslib.citation.volume	63
utslib.location.activity	Germany
utslib.for	0908 Food Sciences
utslib.for	1111 Nutrition and Dietetics
utslib.for	1117 Public Health and Health Services
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-09-14T02:01:42Z
pubs.issue	8
pubs.publication-status	Published
pubs.volume	63
utslib.citation.issue	8

Abstract:

SCOPE: The effects of green tea polyphenols, Polyphenon E (PPE), and black tea polyphenols, theaflavins (TFs), on gut microbiota and development of diabetes in db/db mice are investigated and compared. METHODS AND RESULTS: Supplementation of PPE (0.1%) in the diet of female db/db mice for 7 weeks decreases fasting blood glucose levels and mesenteric fat while increasing the serum level of insulin, possibly through protection against β-cell damage. However, TFs are less or not effective. Microbiome analysis through 16S rRNA gene sequencing shows that PPE and TFs treatments significantly alter the bacterial community structure in the cecum and colon, but not in the ileum. The key bacterial phylotypes responding to the treatments are then clustered into 11 co-abundance groups (CAGs). CAGs 6 and 7, significantly increased by PPE but not by TFs, are negatively associated with blood glucose levels. The operational taxonomic units in these CAGs are from two different phyla, Firmicutes and Bacteroidetes. CAG 10, decreased by PPE and TFs, is positively associated with blood glucose levels. CONCLUSION: Gut microbiota respond to tea polyphenol treatments as CAGs instead of taxa. Some of the CAGs associated with the blood glucose lowering effect are enriched by PPE, but not TFs.

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http://hdl.handle.net/10453/161813