A novel multilocus variable-number tandem repeat analysis for Bordetella parapertussis.

Kamachi, K; Otsuka, N; Fumimoto, R; Ozawa, K; Yao, S-M; Chiang, C-S; Luu, LDW; Lan, R; Shibayama, K; Watanabe, M

A novel multilocus variable-number tandem repeat analysis for Bordetella parapertussis.

Kamachi, K Otsuka, N Fumimoto, R Ozawa, K Yao, S-M Chiang, C-S Luu, LDW Lan, R Shibayama, K Watanabe, M

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Publisher:: MICROBIOLOGY SOC
Publication Type:: Journal Article
Citation:: J Med Microbiol, 2019, 68, (11), pp. 1671-1676
Issue Date:: 2019-11

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Field	Value	Language
dc.contributor.author	Kamachi, K
dc.contributor.author	Otsuka, N
dc.contributor.author	Fumimoto, R
dc.contributor.author	Ozawa, K
dc.contributor.author	Yao, S-M
dc.contributor.author	Chiang, C-S
dc.contributor.author	Luu, LDW
dc.contributor.author	Lan, R
dc.contributor.author	Shibayama, K
dc.contributor.author	Watanabe, M
dc.date.accessioned	2022-09-27T04:56:24Z
dc.date.available	2022-09-27T04:56:24Z
dc.date.issued	2019-11
dc.identifier.citation	J Med Microbiol, 2019, 68, (11), pp. 1671-1676
dc.identifier.issn	0022-2615
dc.identifier.issn	1473-5644
dc.identifier.uri	http://hdl.handle.net/10453/162124
dc.description.abstract	Purpose. Human-adapted Bordetella parapertussis is one of the causative agents of whooping cough; however, there are currently no genotyping systems with high discriminatory power for this bacterial pathogen. We therefore aimed to develop a multilocus variable-number tandem repeat analysis (MLVA) for human-adapted B. parapertussis.Methodology. Four highly polymorphic variable number tandem repeat (VNTR) loci in the B. parapertussis genome were selected and amplified by multiplex PCR. MLVA was performed based on the number of tandem repeats at VNTR loci. The discriminatory power of MLVA was evaluated with three laboratory reference strains and 50 human isolates of B. parapertussis.Results. Multiplex PCR-based MLVA characterized 53 B. parapertussis reference strains and isolates into 25 MLVA types and the Simpson diversity index was 0.91 (95 % confidence interval, 0.86-0.97). The three reference strains exhibited different MLVA types. Thirty-one Japanese isolates, ten French isolates and three Taiwanese isolates belonged to fourteen, nine and three MLVA types, respectively. In contrast, all five Australian isolates belonged to the same type. Two Japanese isolates collected from patients with known epidemiological links had the same type.Conclusion. Our novel MLVA method has high discriminatory power for genotyping human B. parapertussis. Regarding this organism, this genotyping system is a promising tool for epidemiological surveillance and investigating outbreaks.
dc.format	Print
dc.language	eng
dc.publisher	MICROBIOLOGY SOC
dc.relation.ispartof	J Med Microbiol
dc.relation.isbasedon	10.1099/jmm.0.001095
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	06 Biological Sciences, 07 Agricultural and Veterinary Sciences, 11 Medical and Health Sciences
dc.subject.classification	Microbiology
dc.subject.mesh	Bordetella parapertussis
dc.subject.mesh	Humans
dc.subject.mesh	Minisatellite Repeats
dc.subject.mesh	Multilocus Sequence Typing
dc.subject.mesh	Whooping Cough
dc.subject.mesh	Humans
dc.subject.mesh	Bordetella parapertussis
dc.subject.mesh	Whooping Cough
dc.subject.mesh	Minisatellite Repeats
dc.subject.mesh	Multilocus Sequence Typing
dc.title	A novel multilocus variable-number tandem repeat analysis for Bordetella parapertussis.
dc.type	Journal Article
utslib.citation.volume	68
utslib.location.activity	England
utslib.for	06 Biological Sciences
utslib.for	07 Agricultural and Veterinary Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
dc.date.updated	2022-09-27T04:56:20Z
pubs.issue	11
pubs.publication-status	Published
pubs.volume	68
utslib.citation.issue	11

Abstract:

Purpose. Human-adapted Bordetella parapertussis is one of the causative agents of whooping cough; however, there are currently no genotyping systems with high discriminatory power for this bacterial pathogen. We therefore aimed to develop a multilocus variable-number tandem repeat analysis (MLVA) for human-adapted B. parapertussis.Methodology. Four highly polymorphic variable number tandem repeat (VNTR) loci in the B. parapertussis genome were selected and amplified by multiplex PCR. MLVA was performed based on the number of tandem repeats at VNTR loci. The discriminatory power of MLVA was evaluated with three laboratory reference strains and 50 human isolates of B. parapertussis.Results. Multiplex PCR-based MLVA characterized 53 B. parapertussis reference strains and isolates into 25 MLVA types and the Simpson diversity index was 0.91 (95 % confidence interval, 0.86-0.97). The three reference strains exhibited different MLVA types. Thirty-one Japanese isolates, ten French isolates and three Taiwanese isolates belonged to fourteen, nine and three MLVA types, respectively. In contrast, all five Australian isolates belonged to the same type. Two Japanese isolates collected from patients with known epidemiological links had the same type.Conclusion. Our novel MLVA method has high discriminatory power for genotyping human B. parapertussis. Regarding this organism, this genotyping system is a promising tool for epidemiological surveillance and investigating outbreaks.

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http://hdl.handle.net/10453/162124