Signal amplification of araC pBAD using a standardized translation initiation region.

Shilling, PJ; Khananisho, D; Cumming, AJ; Söderström, B; Daley, DO

Signal amplification of araC pBAD using a standardized translation initiation region.

Shilling, PJ Khananisho, D Cumming, AJ Söderström, B Daley, DO

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Publisher:: Oxford University Press (OUP)
Publication Type:: Journal Article
Citation:: Synth Biol (Oxf), 2022, 7, (1), pp. ysac009
Issue Date:: 2022

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Field	Value	Language
dc.contributor.author	Shilling, PJ
dc.contributor.author	Khananisho, D
dc.contributor.author	Cumming, AJ
dc.contributor.author	Söderström, B
dc.contributor.author	Daley, DO
dc.date.accessioned	2023-01-04T06:32:22Z
dc.date.available	2022-07-04
dc.date.available	2023-01-04T06:32:22Z
dc.date.issued	2022
dc.identifier.citation	Synth Biol (Oxf), 2022, 7, (1), pp. ysac009
dc.identifier.issn	2397-7000
dc.identifier.issn	2397-7000
dc.identifier.uri	http://hdl.handle.net/10453/164687
dc.description.abstract	araC pBAD is a genetic fragment that regulates the expression of the araBAD operon in bacteria, which is required for the metabolism of L-arabinose. It is widely used in bioengineering applications because it can drive regulatable and titratable expression of genes and genetic pathways in microbial cell factories. A notable limitation of araC pBAD is that it generates a low signal when induced with high concentrations of L-arabinose (the maximum ON state). Herein we have amplified the maximum ON state of araC pBAD by coupling it to a synthetically evolved translation initiation region (TIREVOL ). The coupling maintains regulatable and titratable expression from araC pBAD and yet increases the maximal ON state by >5-fold. The general principle demonstrated in the study can be applied to amplify the signal from similar genetic modules. Graphical Abstract.
dc.format	Electronic-eCollection
dc.language	eng
dc.publisher	Oxford University Press (OUP)
dc.relation	http://purl.org/au-research/grants/arc/DP220101143
dc.relation.ispartof	Synth Biol (Oxf)
dc.relation.isbasedon	10.1093/synbio/ysac009
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Signal amplification of araC pBAD using a standardized translation initiation region.
dc.type	Journal Article
utslib.citation.volume	7
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
utslib.copyright.status	open_access	*
dc.date.updated	2023-01-04T06:32:01Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	7
utslib.citation.issue	1

Abstract:

araC pBAD is a genetic fragment that regulates the expression of the araBAD operon in bacteria, which is required for the metabolism of L-arabinose. It is widely used in bioengineering applications because it can drive regulatable and titratable expression of genes and genetic pathways in microbial cell factories. A notable limitation of araC pBAD is that it generates a low signal when induced with high concentrations of L-arabinose (the maximum ON state). Herein we have amplified the maximum ON state of araC pBAD by coupling it to a synthetically evolved translation initiation region (TIREVOL ). The coupling maintains regulatable and titratable expression from araC pBAD and yet increases the maximal ON state by >5-fold. The general principle demonstrated in the study can be applied to amplify the signal from similar genetic modules. Graphical Abstract.

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http://hdl.handle.net/10453/164687