Analysis of pCl107 a large plasmid carried by an ST25 Acinetobacter baumannii strain reveals a complex evolutionary history and links to multiple antibiotic resistance and metabolic pathways

Rafei, R; Koong, J; Osman, M; Atrouni, AA; Hamze, M; Hamidian, M

Analysis of pCl107 a large plasmid carried by an ST25 Acinetobacter baumannii strain reveals a complex evolutionary history and links to multiple antibiotic resistance and metabolic pathways

Rafei, R Koong, J Osman, M Atrouni, AA Hamze, M Hamidian, M

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Publisher:: Oxford University Press (OUP)
Publication Type:: Journal Article
Citation:: FEMS Microbes, 2022, 3, pp. xtac027
Issue Date:: 2022-11-18

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Field	Value	Language
dc.contributor.author	Rafei, R
dc.contributor.author	Koong, J
dc.contributor.author	Osman, M
dc.contributor.author	Atrouni, AA
dc.contributor.author	Hamze, M
dc.contributor.author	Hamidian, M https://orcid.org/0000-0002-3614-7261
dc.date.accessioned	2023-01-04T07:01:55Z
dc.date.available	2023-01-04T07:01:55Z
dc.date.issued	2022-11-18
dc.identifier.citation	FEMS Microbes, 2022, 3, pp. xtac027
dc.identifier.issn	2633-6685
dc.identifier.issn	2633-6685
dc.identifier.uri	http://hdl.handle.net/10453/164688
dc.description.abstract	<jats:title>Abstract</jats:title> <jats:p>Acinetobacter baumannii has successfully spread during the last decades as one of the main critically important pathogens. However, many aspects including plasmids, are still under-investigated. Here, we report the complete sequence of an Acinetobacter baumannii strain, belonging to the ST25IP (Institut Pasteur) sequence type recovered in 2012 in Lebanon, using a combination of Illumina MiSeq and Oxford Nanopore sequencing and a hybrid assembly approach. This strain (Cl107) carries a 198 kb plasmid called pCl107 that encodes the MPFI conjugative transfer system. The plasmid carries the aacA1, aacC2, sul2, strAB, and tetA(B) antibiotic resistance genes. pCl107 region encompassing the sul2, strAB, tetA(B) is closely related to AbGRI1 chromosomal resistance islands, which are widespread in A. baumannii strains belonging to Global Clone 2. The resistance region found in pCl107 is one of the missing links in the evolutionary history of the AbGRI1 islands. pCl107 also contains a BREX Type 1 region and represents one of the two main evolution patterns observed in BREX clusters found in plasmids related to pCl107. pCl107 also harbours a ptx phosphonate metabolism module, which plays an ancestral structure compared to other large plasmids in ST25 strains. While the uric acid metabolic module found in pCl107 is incomplete, we identified possible ancestors from plasmids and chromosomes of Acinetobacter spp. Our analyses indicate a complex evolutionary history of plasmids related to pCl107 with many links to multiple antibiotic resistance and metabolic pathways.</jats:p>
dc.language	en
dc.publisher	Oxford University Press (OUP)
dc.relation	http://purl.org/au-research/grants/arc/DE200100111
dc.relation.ispartof	FEMS Microbes
dc.relation.isbasedon	10.1093/femsmc/xtac027
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Analysis of pCl107 a large plasmid carried by an ST25 Acinetobacter baumannii strain reveals a complex evolutionary history and links to multiple antibiotic resistance and metabolic pathways
dc.type	Journal Article
utslib.citation.volume	3
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
utslib.copyright.status	open_access	*
dc.date.updated	2023-01-04T07:01:34Z
pubs.publication-status	Published
pubs.volume	3

Abstract:

Abstract Acinetobacter baumannii has successfully spread during the last decades as one of the main critically important pathogens. However, many aspects including plasmids, are still under-investigated. Here, we report the complete sequence of an Acinetobacter baumannii strain, belonging to the ST25IP (Institut Pasteur) sequence type recovered in 2012 in Lebanon, using a combination of Illumina MiSeq and Oxford Nanopore sequencing and a hybrid assembly approach. This strain (Cl107) carries a 198 kb plasmid called pCl107 that encodes the MPFI conjugative transfer system. The plasmid carries the aacA1, aacC2, sul2, strAB, and tetA(B) antibiotic resistance genes. pCl107 region encompassing the sul2, strAB, tetA(B) is closely related to AbGRI1 chromosomal resistance islands, which are widespread in A. baumannii strains belonging to Global Clone 2. The resistance region found in pCl107 is one of the missing links in the evolutionary history of the AbGRI1 islands. pCl107 also contains a BREX Type 1 region and represents one of the two main evolution patterns observed in BREX clusters found in plasmids related to pCl107. pCl107 also harbours a ptx phosphonate metabolism module, which plays an ancestral structure compared to other large plasmids in ST25 strains. While the uric acid metabolic module found in pCl107 is incomplete, we identified possible ancestors from plasmids and chromosomes of Acinetobacter spp. Our analyses indicate a complex evolutionary history of plasmids related to pCl107 with many links to multiple antibiotic resistance and metabolic pathways.

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http://hdl.handle.net/10453/164688