Fluorescence in situ hybridization and microbial community profiling analysis of explanted cochlear implants.

Asfour, L; Smyth, D; Whitchurch, CB; Cavaliere, R; Roland, JT

Fluorescence in situ hybridization and microbial community profiling analysis of explanted cochlear implants.

Asfour, L Smyth, D Whitchurch, CB Cavaliere, R

Roland, JT

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Publisher:: Taylor & Francis
Publication Type:: Journal Article
Citation:: Acta Otolaryngol, 2022, 142, (5), pp. 395-401
Issue Date:: 2022-05

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Field	Value	Language
dc.contributor.author	Asfour, L
dc.contributor.author	Smyth, D
dc.contributor.author	Whitchurch, CB
dc.contributor.author	Cavaliere, R https://orcid.org/0000-0002-4709-5081
dc.contributor.author	Roland, JT
dc.date.accessioned	2023-01-30T22:24:56Z
dc.date.available	2023-01-30T22:24:56Z
dc.date.issued	2022-05
dc.identifier.citation	Acta Otolaryngol, 2022, 142, (5), pp. 395-401
dc.identifier.issn	0001-6489
dc.identifier.issn	1651-2251
dc.identifier.uri	http://hdl.handle.net/10453/165628
dc.description.abstract	BACKGROUND: Cochlear implant (CI) infections affect a small, but significant number of patients. Unremitting infections can lead to explantation. Fluorescence in situ hybridization (FISH) and microbial community profiling (MCP) are methods of studying microbial environments of explanted devices that can provide information to reduce morbidity and costs of infected CIs. AIMS/OBJECTIVES: To describe the results and clinical significance of bacterial analyses conducted on explanted CIs. MATERIAL AND METHODS: Between 2013 and 2017, 12 explanted devices underwent microbiological analysis in addition to the manufacturer's device failure analysis. Patients' clinical history, infection status and outcome were reviewed and correlated with microbial analysis results. RESULTS: From 2013 to 2017, 12 Cochlear™ devices from 11 patients underwent additional MCP or FISH analysis. Five devices were explanted due to suspected implant associated infection, and seven were explanted for other reasons. FISH analysis revealed biofilm presence on all infected devices, only partial correlation of cultures with biofilm composition and confirmation that biofilm formation occurs preferentially at particular device interfaces and geometries. MCP analysis presented challenges in data analysis inherent to its technique but correlated with cultures of infected devices and suggested a diverse microbial composition of explanted devices. CONCLUSIONS AND SIGNIFICANCE: Microbial analysis of explanted devices can aid in further elucidating treatment approaches to infected CIs.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Taylor & Francis
dc.relation.ispartof	Acta Otolaryngol
dc.relation.isbasedon	10.1080/00016489.2022.2070931
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	1103 Clinical Sciences
dc.subject.classification	Otorhinolaryngology
dc.subject.classification	Public Health
dc.subject.mesh	Biofilms
dc.subject.mesh	Cochlear Implantation
dc.subject.mesh	Cochlear Implants
dc.subject.mesh	Humans
dc.subject.mesh	In Situ Hybridization, Fluorescence
dc.subject.mesh	Microbiota
dc.subject.mesh	Postoperative Complications
dc.subject.mesh	Humans
dc.subject.mesh	Biofilms
dc.subject.mesh	Postoperative Complications
dc.subject.mesh	Cochlear Implantation
dc.subject.mesh	In Situ Hybridization, Fluorescence
dc.subject.mesh	Cochlear Implants
dc.subject.mesh	Microbiota
dc.subject.mesh	Biofilms
dc.subject.mesh	Cochlear Implantation
dc.subject.mesh	Cochlear Implants
dc.subject.mesh	Humans
dc.subject.mesh	In Situ Hybridization, Fluorescence
dc.subject.mesh	Microbiota
dc.subject.mesh	Postoperative Complications
dc.title	Fluorescence in situ hybridization and microbial community profiling analysis of explanted cochlear implants.
dc.type	Journal Article
utslib.citation.volume	142
utslib.location.activity	England
utslib.for	1103 Clinical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
utslib.copyright.status	closed_access	*
dc.date.updated	2023-01-30T22:24:54Z
pubs.issue	5
pubs.publication-status	Published
pubs.volume	142
utslib.citation.issue	5

Abstract:

BACKGROUND: Cochlear implant (CI) infections affect a small, but significant number of patients. Unremitting infections can lead to explantation. Fluorescence in situ hybridization (FISH) and microbial community profiling (MCP) are methods of studying microbial environments of explanted devices that can provide information to reduce morbidity and costs of infected CIs. AIMS/OBJECTIVES: To describe the results and clinical significance of bacterial analyses conducted on explanted CIs. MATERIAL AND METHODS: Between 2013 and 2017, 12 explanted devices underwent microbiological analysis in addition to the manufacturer's device failure analysis. Patients' clinical history, infection status and outcome were reviewed and correlated with microbial analysis results. RESULTS: From 2013 to 2017, 12 Cochlear™ devices from 11 patients underwent additional MCP or FISH analysis. Five devices were explanted due to suspected implant associated infection, and seven were explanted for other reasons. FISH analysis revealed biofilm presence on all infected devices, only partial correlation of cultures with biofilm composition and confirmation that biofilm formation occurs preferentially at particular device interfaces and geometries. MCP analysis presented challenges in data analysis inherent to its technique but correlated with cultures of infected devices and suggested a diverse microbial composition of explanted devices. CONCLUSIONS AND SIGNIFICANCE: Microbial analysis of explanted devices can aid in further elucidating treatment approaches to infected CIs.

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http://hdl.handle.net/10453/165628