Characterization of the Wave Phenomenon in Flash-Induced Fluorescence Relaxation and Its Application to Study Cyclic Electron Pathways in Microalgae.

Patil, PP; Vass, I; Szabó, M

Characterization of the Wave Phenomenon in Flash-Induced Fluorescence Relaxation and Its Application to Study Cyclic Electron Pathways in Microalgae.

Patil, PP Vass, I Szabó, M

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Int J Mol Sci, 2022, 23, (9), pp. 4927
Issue Date:: 2022-04-28

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Field	Value	Language
dc.contributor.author	Patil, PP
dc.contributor.author	Vass, I
dc.contributor.author	Szabó, M
dc.date.accessioned	2023-01-31T23:51:47Z
dc.date.available	2022-04-26
dc.date.available	2023-01-31T23:51:47Z
dc.date.issued	2022-04-28
dc.identifier.citation	Int J Mol Sci, 2022, 23, (9), pp. 4927
dc.identifier.issn	1422-0067
dc.identifier.issn	1422-0067
dc.identifier.uri	http://hdl.handle.net/10453/165713
dc.description.abstract	Photosynthesis is a series of redox reactions, in which several electron transport processes operate to provide the energetic balance of light harvesting. In addition to linear electron flow, which ensures the basic functions of photosynthetic productivity and carbon fixation, alternative electron transport pathways operate, such as the cyclic electron flow (CEF), which play a role in the fine tuning of photosynthesis and balancing the ATP/NADPH ratio under stress conditions. In this work, we characterized the electron transport processes in microalgae species that have high relevance in applied research and industry (e.g., Chlorella sorokiniana, Haematococcus pluvialis, Dunaliella salina, Nannochloropsis sp.) by using flash-induced fluorescence relaxation kinetics. We found that a wave phenomenon appeared in the fluorescence relaxation profiles of microalgae to different extents; it was remarkable in the red cells of H. pluvialis, D. salina and C. sorokiniana, but it was absent in green cells of H. pluvialis and N. limnetica. Furthermore, in microalgae, unlike in cyanobacteria, the appearance of the wave required the partial decrease in the activity of Photosystem II, because the relatively high Photosystem II/Photosystem I ratio in microalgae prevented the enhanced oxidation of the plastoquinone pool. The wave phenomenon was shown to be related to the antimycin A-sensitive pathway of CEF in C. sorokiniana but not in other species. Therefore, the fluorescence wave phenomenon appears to be a species-specific indicator of the redox reactions of the plastoquinone pool and certain pathways of cyclic electron flow.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation.ispartof	Int J Mol Sci
dc.relation.isbasedon	10.3390/ijms23094927
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0399 Other Chemical Sciences, 0604 Genetics, 0699 Other Biological Sciences
dc.subject.classification	Chemical Physics
dc.subject.mesh	Chlorella
dc.subject.mesh	Chlorophyll
dc.subject.mesh	Electron Transport
dc.subject.mesh	Electrons
dc.subject.mesh	Fluorescence
dc.subject.mesh	Microalgae
dc.subject.mesh	Photosynthesis
dc.subject.mesh	Photosystem I Protein Complex
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	Plastoquinone
dc.subject.mesh	Chlorella
dc.subject.mesh	Plastoquinone
dc.subject.mesh	Chlorophyll
dc.subject.mesh	Photosystem I Protein Complex
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	Photosynthesis
dc.subject.mesh	Electron Transport
dc.subject.mesh	Electrons
dc.subject.mesh	Fluorescence
dc.subject.mesh	Microalgae
dc.title	Characterization of the Wave Phenomenon in Flash-Induced Fluorescence Relaxation and Its Application to Study Cyclic Electron Pathways in Microalgae.
dc.type	Journal Article
utslib.citation.volume	23
utslib.location.activity	Switzerland
utslib.for	0399 Other Chemical Sciences
utslib.for	0604 Genetics
utslib.for	0699 Other Biological Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2023-01-31T23:51:42Z
pubs.issue	9
pubs.publication-status	Published online
pubs.volume	23
utslib.citation.issue	9

Abstract:

Photosynthesis is a series of redox reactions, in which several electron transport processes operate to provide the energetic balance of light harvesting. In addition to linear electron flow, which ensures the basic functions of photosynthetic productivity and carbon fixation, alternative electron transport pathways operate, such as the cyclic electron flow (CEF), which play a role in the fine tuning of photosynthesis and balancing the ATP/NADPH ratio under stress conditions. In this work, we characterized the electron transport processes in microalgae species that have high relevance in applied research and industry (e.g., Chlorella sorokiniana, Haematococcus pluvialis, Dunaliella salina, Nannochloropsis sp.) by using flash-induced fluorescence relaxation kinetics. We found that a wave phenomenon appeared in the fluorescence relaxation profiles of microalgae to different extents; it was remarkable in the red cells of H. pluvialis, D. salina and C. sorokiniana, but it was absent in green cells of H. pluvialis and N. limnetica. Furthermore, in microalgae, unlike in cyanobacteria, the appearance of the wave required the partial decrease in the activity of Photosystem II, because the relatively high Photosystem II/Photosystem I ratio in microalgae prevented the enhanced oxidation of the plastoquinone pool. The wave phenomenon was shown to be related to the antimycin A-sensitive pathway of CEF in C. sorokiniana but not in other species. Therefore, the fluorescence wave phenomenon appears to be a species-specific indicator of the redox reactions of the plastoquinone pool and certain pathways of cyclic electron flow.

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http://hdl.handle.net/10453/165713