From Differential DNA Methylation in COPD to Mitochondria: Regulation of AHRR Expression Affects Airway Epithelial Response to Cigarette Smoke.
Chen, Q
Nwozor, KO
van den Berge, M
Slebos, D-J
Faiz, A
Jonker, MR
Boezen, HM
Heijink, IH
de Vries, M
- Publisher:
- MDPI
- Publication Type:
- Journal Article
- Citation:
- Cells, 2022, 11, (21), pp. 3423
- Issue Date:
- 2022-10-29
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, Q | |
dc.contributor.author | Nwozor, KO | |
dc.contributor.author | van den Berge, M | |
dc.contributor.author | Slebos, D-J | |
dc.contributor.author |
Faiz, A https://orcid.org/0000-0003-1740-3538 |
|
dc.contributor.author | Jonker, MR | |
dc.contributor.author | Boezen, HM | |
dc.contributor.author | Heijink, IH | |
dc.contributor.author | de Vries, M | |
dc.date.accessioned | 2023-02-03T00:24:53Z | |
dc.date.available | 2022-10-27 | |
dc.date.available | 2023-02-03T00:24:53Z | |
dc.date.issued | 2022-10-29 | |
dc.identifier.citation | Cells, 2022, 11, (21), pp. 3423 | |
dc.identifier.issn | 2073-4409 | |
dc.identifier.issn | 2073-4409 | |
dc.identifier.uri | http://hdl.handle.net/10453/165871 | |
dc.description.abstract | Cigarette smoking causes hypomethylation of the gene Aryl Hydrocarbon Receptor Repressor (AHRR), which regulates detoxification and oxidative stress-responses. We investigated whether AHRR DNA methylation is related to chronic obstructive pulmonary disease (COPD) and studied its function in airway epithelial cells (AECs). The association with COPD was assessed in blood from never and current smokers with/without COPD, and in AECs from ex-smoking non-COPD controls and GOLD stage II-IV COPD patients cultured with/without cigarette smoke extract (CSE). The effect of CRISPR/Cas9-induced AHRR knockout on proliferation, CSE-induced mitochondrial membrane potential and apoptosis/necrosis in human bronchial epithelial 16HBE cells was studied. In blood, DNA methylation of AHRR at cg05575921 and cg21161138 was lower in smoking COPD subjects than smoking controls. In vitro, AHRR DNA methylation at these CpG-sites was lower in COPD-derived than control-derived AECs only upon CSE exposure. Upon AHRR knockout, we found a lower proliferation rate at baseline, stronger CSE-induced decrease in mitochondrial membrane potential, and higher CSE-induced late apoptosis/necroptosis. Together, our results show lower DNA methylation of AHRR upon smoking in COPD patients compared to non-COPD controls. Our data suggest that higher airway epithelial AHRR expression may lead to impaired cigarette smoke-induced mitochondrial dysfunction and apoptosis/necroptosis, potentially promoting unprogrammed/immunogenic cell death. | |
dc.format | Electronic | |
dc.language | eng | |
dc.publisher | MDPI | |
dc.relation.ispartof | Cells | |
dc.relation.isbasedon | 10.3390/cells11213423 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Basic Helix-Loop-Helix Transcription Factors | |
dc.subject.mesh | Cigarette Smoking | |
dc.subject.mesh | DNA Methylation | |
dc.subject.mesh | Mitochondria | |
dc.subject.mesh | Pulmonary Disease, Chronic Obstructive | |
dc.subject.mesh | Receptors, Aryl Hydrocarbon | |
dc.subject.mesh | Repressor Proteins | |
dc.subject.mesh | Tobacco | |
dc.subject.mesh | Mitochondria | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Tobacco | |
dc.subject.mesh | Pulmonary Disease, Chronic Obstructive | |
dc.subject.mesh | Receptors, Aryl Hydrocarbon | |
dc.subject.mesh | Repressor Proteins | |
dc.subject.mesh | DNA Methylation | |
dc.subject.mesh | Basic Helix-Loop-Helix Transcription Factors | |
dc.subject.mesh | Cigarette Smoking | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Basic Helix-Loop-Helix Transcription Factors | |
dc.subject.mesh | Cigarette Smoking | |
dc.subject.mesh | DNA Methylation | |
dc.subject.mesh | Mitochondria | |
dc.subject.mesh | Pulmonary Disease, Chronic Obstructive | |
dc.subject.mesh | Receptors, Aryl Hydrocarbon | |
dc.subject.mesh | Repressor Proteins | |
dc.subject.mesh | Tobacco | |
dc.title | From Differential DNA Methylation in COPD to Mitochondria: Regulation of AHRR Expression Affects Airway Epithelial Response to Cigarette Smoke. | |
dc.type | Journal Article | |
utslib.citation.volume | 11 | |
utslib.location.activity | Switzerland | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Life Sciences | |
pubs.organisational-group | /University of Technology Sydney/Centre for Health Technologies (CHT) | |
pubs.organisational-group | /University of Technology Sydney/Strength - CFI - Centre for Inflammation | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2023-02-03T00:24:47Z | |
pubs.issue | 21 | |
pubs.publication-status | Published online | |
pubs.volume | 11 | |
utslib.citation.issue | 21 |
Abstract:
Cigarette smoking causes hypomethylation of the gene Aryl Hydrocarbon Receptor Repressor (AHRR), which regulates detoxification and oxidative stress-responses. We investigated whether AHRR DNA methylation is related to chronic obstructive pulmonary disease (COPD) and studied its function in airway epithelial cells (AECs). The association with COPD was assessed in blood from never and current smokers with/without COPD, and in AECs from ex-smoking non-COPD controls and GOLD stage II-IV COPD patients cultured with/without cigarette smoke extract (CSE). The effect of CRISPR/Cas9-induced AHRR knockout on proliferation, CSE-induced mitochondrial membrane potential and apoptosis/necrosis in human bronchial epithelial 16HBE cells was studied. In blood, DNA methylation of AHRR at cg05575921 and cg21161138 was lower in smoking COPD subjects than smoking controls. In vitro, AHRR DNA methylation at these CpG-sites was lower in COPD-derived than control-derived AECs only upon CSE exposure. Upon AHRR knockout, we found a lower proliferation rate at baseline, stronger CSE-induced decrease in mitochondrial membrane potential, and higher CSE-induced late apoptosis/necroptosis. Together, our results show lower DNA methylation of AHRR upon smoking in COPD patients compared to non-COPD controls. Our data suggest that higher airway epithelial AHRR expression may lead to impaired cigarette smoke-induced mitochondrial dysfunction and apoptosis/necroptosis, potentially promoting unprogrammed/immunogenic cell death.
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