Proteomic Analysis of Whole Blood Using Volumetric Absorptive Microsampling for Precision Medicine Biomarker Studies.

Molloy, MP; Hill, C; O'Rourke, MB; Chandra, J; Steffen, P; McKay, MJ; Pascovici, D; Herbert, BR

Proteomic Analysis of Whole Blood Using Volumetric Absorptive Microsampling for Precision Medicine Biomarker Studies.

Molloy, MP Hill, C O'Rourke, MB Chandra, J Steffen, P McKay, MJ Pascovici, D Herbert, BR

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: J Proteome Res, 2022, 21, (4), pp. 1196-1203
Issue Date:: 2022-04-01

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Field	Value	Language
dc.contributor.author	Molloy, MP
dc.contributor.author	Hill, C
dc.contributor.author	O'Rourke, MB
dc.contributor.author	Chandra, J
dc.contributor.author	Steffen, P
dc.contributor.author	McKay, MJ
dc.contributor.author	Pascovici, D
dc.contributor.author	Herbert, BR
dc.date.accessioned	2023-02-08T04:21:28Z
dc.date.available	2023-02-08T04:21:28Z
dc.date.issued	2022-04-01
dc.identifier.citation	J Proteome Res, 2022, 21, (4), pp. 1196-1203
dc.identifier.issn	1535-3893
dc.identifier.issn	1535-3907
dc.identifier.uri	http://hdl.handle.net/10453/166004
dc.description.abstract	Microsampling of patient blood promises several benefits over conventional phlebotomy practices to facilitate precision medicine studies. These include at-home patient blood collection, supporting telehealth monitoring, minimal postcollection processing, and compatibility with nonrefrigerated transport and storage. However, for proteomic biomarker studies, mass spectrometry of whole blood has generally been avoided in favor of using plasma or serum obtained from venepuncture. We evaluated the use of a volumetric absorptive microsampling (VAMS) device as a sample preparation matrix to enable LC-MS proteomic analyses of dried whole blood. We demonstrated the detection and robust quantitation of up to 1600 proteins from single-shot shotgun-LC-MS analysis of dried whole blood, greatly enhancing proteome depth compared with conventional single-shot LC-MS analyses of undepleted plasma. Some proteins not previously reported in blood were detected using this approach. Various washing reagents were used to demonstrate that proteins can be preferentially removed from VAMS devices prior to downstream analyses. We provide a demonstration that archival frozen blood cell pellets housed under long-term storage (exceeding 5 years) are compatible with VAMS to enable quantitation of potential biomarker proteins from biobank repositories. These demonstrations are important steps in establishing viable analysis workflows to underpin large-scale precision medicine studies. Data are available via ProteomeXchange with the identifier PXD028605.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	J Proteome Res
dc.relation.isbasedon	10.1021/acs.jproteome.1c00971
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 06 Biological Sciences
dc.subject.classification	Biochemistry & Molecular Biology
dc.subject.mesh	Biomarkers
dc.subject.mesh	Blood Specimen Collection
dc.subject.mesh	Dried Blood Spot Testing
dc.subject.mesh	Humans
dc.subject.mesh	Precision Medicine
dc.subject.mesh	Proteomics
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Humans
dc.subject.mesh	Blood Specimen Collection
dc.subject.mesh	Proteomics
dc.subject.mesh	Tandem Mass Spectrometry
dc.subject.mesh	Dried Blood Spot Testing
dc.subject.mesh	Biomarkers
dc.subject.mesh	Precision Medicine
dc.title	Proteomic Analysis of Whole Blood Using Volumetric Absorptive Microsampling for Precision Medicine Biomarker Studies.
dc.type	Journal Article
utslib.citation.volume	21
utslib.location.activity	United States
utslib.for	03 Chemical Sciences
utslib.for	06 Biological Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFI - Centre for Inflammation
utslib.copyright.status	closed_access	*
dc.date.updated	2023-02-08T04:21:27Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	21
utslib.citation.issue	4

Abstract:

Microsampling of patient blood promises several benefits over conventional phlebotomy practices to facilitate precision medicine studies. These include at-home patient blood collection, supporting telehealth monitoring, minimal postcollection processing, and compatibility with nonrefrigerated transport and storage. However, for proteomic biomarker studies, mass spectrometry of whole blood has generally been avoided in favor of using plasma or serum obtained from venepuncture. We evaluated the use of a volumetric absorptive microsampling (VAMS) device as a sample preparation matrix to enable LC-MS proteomic analyses of dried whole blood. We demonstrated the detection and robust quantitation of up to 1600 proteins from single-shot shotgun-LC-MS analysis of dried whole blood, greatly enhancing proteome depth compared with conventional single-shot LC-MS analyses of undepleted plasma. Some proteins not previously reported in blood were detected using this approach. Various washing reagents were used to demonstrate that proteins can be preferentially removed from VAMS devices prior to downstream analyses. We provide a demonstration that archival frozen blood cell pellets housed under long-term storage (exceeding 5 years) are compatible with VAMS to enable quantitation of potential biomarker proteins from biobank repositories. These demonstrations are important steps in establishing viable analysis workflows to underpin large-scale precision medicine studies. Data are available via ProteomeXchange with the identifier PXD028605.

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http://hdl.handle.net/10453/166004