The immobilization of Candida rugosa lipase on the modified polyethersulfone with MOF nanoparticles as an excellent performance bioreactor membrane.
- Publisher:
- ELSEVIER SCIENCE BV
- Publication Type:
- Journal Article
- Citation:
- J Biotechnol, 2019, 289, pp. 55-63
- Issue Date:
- 2019-01-10
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| Filename | Description | Size | |||
|---|---|---|---|---|---|
| The immobilization of Candida rugosa lipase on the modified polyethersulfone with MOF nanoparticles as an excellent performance bioreactor membrane..pdf | Published version | 2.46 MB |
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In this study, the modified nanocomposite membrane of polyethersulfone (PES) with NH2-MIL-101(Cr) as a metal-organic framework (MOF) is exploited for Candida rugosa lipase (CRL) immobilization. To that end, the various amounts of NH2-MIL-101(Cr) nanoparticles are blended into PES casting solution to fabricate ultrafiltration membrane via phase inversion technique. The incorporation efficiency of NH2-MIL-101(Cr) nanoparticles on the membrane morphology is investigated using various techniques, namely atomic force microscopy (AFM), X-ray diffraction (XRD), and contact angle goniometry. In terms of water pure flux and CRL immobilization efficiency, the best performance is observed for PES-NH2-MIL1% membrane. This bioactive membrane (CRL@GA@PES-NH2-MIL1%) displays an improvement in pH and thermal stability and separation performance that makes it a fruitful candidate for using in bioreactors. The examination of the wet- and dry-storage stability of CRL@GA@PES-NH2-MIL1% demonstrates the high stability for the wet bioactive membrane. The reusability inspection of CRL@GA@PES-NH2-MIL1% represents about 50% conservation of the residual activity after 12 sequential usage cycles.
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