Multienzymatic conversion of monosaccharides from birch biomass after pretreatment

Bachosz, K; Zdarta, J; Nghiem, LD; Jesionowski, T

Multienzymatic conversion of monosaccharides from birch biomass after pretreatment

Bachosz, K Zdarta, J Nghiem, LD Jesionowski, T

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Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: Environmental Technology and Innovation, 2022, 28
Issue Date:: 2022-11-01

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Field	Value	Language
dc.contributor.author	Bachosz, K
dc.contributor.author	Zdarta, J
dc.contributor.author	Nghiem, LD
dc.contributor.author	Jesionowski, T
dc.date.accessioned	2023-04-11T05:16:56Z
dc.date.available	2023-04-11T05:16:56Z
dc.date.issued	2022-11-01
dc.identifier.citation	Environmental Technology and Innovation, 2022, 28
dc.identifier.issn	2352-1864
dc.identifier.issn	2352-1864
dc.identifier.uri	http://hdl.handle.net/10453/169593
dc.description.abstract	This study presents proof-of-concept validation of a multi enzymatic biocatalytic system, capable of producing monosaccharides from a birch biomass pretreated liquor with simultaneous cofactor regeneration. To improve the stability of the proposed biocatalyst, co-immobilization of NAD+-dependent xylose dehydrogenases, glucose dehydrogenase, and NADH-dependent 3-hydroxybutyrate dehydrogenase was performed on mesoporous silica SBA-15. The results of the physicochemical analysis confirmed the effectiveness of enzymes deposition on the support material and changes in the surface area and porous structure of silica before and after the process. Process conditions in which the three-enzymes system allowed to achieve the highest efficiency of the conducted reactions were optimized at 20 °C, pH 7, 90 min of process duration, 400 mM of levulinic acid addition and ratio of NAD+:NADH 1:5. Results from this study demonstrate excellent xylonic acid, gluconic acid and 4-hydroxyvaleric acid production at conversion rate of 98.7%, 95.6% and 99.2%, respectively. In addition, the high stability and reusability of the developed biocatalytic system was confirmed based on tests in ten successive catalytic cycles and the storage time of 10 days at 4 °C. At the end of the tests, co-immobilized enzymes were capable of catalyzing the process with >70% efficiency. The presented data confirmed the effectiveness of co-immobilization, improvement of enzyme properties including their high reusability, as well as indicated the application potential of the proposed multienzymatic system in the conversion of components of pretreated biomass solutions.
dc.language	English
dc.publisher	ELSEVIER
dc.relation.ispartof	Environmental Technology and Innovation
dc.relation.isbasedon	10.1016/j.eti.2022.102874
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0502 Environmental Science and Management, 0907 Environmental Engineering, 1002 Environmental Biotechnology
dc.title	Multienzymatic conversion of monosaccharides from birch biomass after pretreatment
dc.type	Journal Article
utslib.citation.volume	28
utslib.for	0502 Environmental Science and Management
utslib.for	0907 Environmental Engineering
utslib.for	1002 Environmental Biotechnology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	open_access	*
dc.date.updated	2023-04-11T05:16:55Z
pubs.publication-status	Published
pubs.volume	28

Abstract:

This study presents proof-of-concept validation of a multi enzymatic biocatalytic system, capable of producing monosaccharides from a birch biomass pretreated liquor with simultaneous cofactor regeneration. To improve the stability of the proposed biocatalyst, co-immobilization of NAD+-dependent xylose dehydrogenases, glucose dehydrogenase, and NADH-dependent 3-hydroxybutyrate dehydrogenase was performed on mesoporous silica SBA-15. The results of the physicochemical analysis confirmed the effectiveness of enzymes deposition on the support material and changes in the surface area and porous structure of silica before and after the process. Process conditions in which the three-enzymes system allowed to achieve the highest efficiency of the conducted reactions were optimized at 20 °C, pH 7, 90 min of process duration, 400 mM of levulinic acid addition and ratio of NAD+:NADH 1:5. Results from this study demonstrate excellent xylonic acid, gluconic acid and 4-hydroxyvaleric acid production at conversion rate of 98.7%, 95.6% and 99.2%, respectively. In addition, the high stability and reusability of the developed biocatalytic system was confirmed based on tests in ten successive catalytic cycles and the storage time of 10 days at 4 °C. At the end of the tests, co-immobilized enzymes were capable of catalyzing the process with >70% efficiency. The presented data confirmed the effectiveness of co-immobilization, improvement of enzyme properties including their high reusability, as well as indicated the application potential of the proposed multienzymatic system in the conversion of components of pretreated biomass solutions.

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http://hdl.handle.net/10453/169593