Field |
Value |
Language |
dc.contributor.author |
Chhor, M |
|
dc.contributor.author |
Tulpar, E |
|
dc.contributor.author |
Nguyen, T |
|
dc.contributor.author |
Cranfield, CG |
|
dc.contributor.author |
Gorrie, CA |
|
dc.contributor.author |
Chan, YL |
|
dc.contributor.author |
Chen, H
https://orcid.org/0000-0001-6883-3752
|
|
dc.contributor.author |
Oliver, BG |
|
dc.contributor.author |
McClements, L
https://orcid.org/0000-0002-4911-1014
|
|
dc.contributor.author |
McGrath, KC |
|
dc.date.accessioned |
2023-04-18T03:47:50Z |
|
dc.date.available |
2023-04-18T03:47:50Z |
|
dc.identifier.citation |
International Journal of Molecular Sciences, 24, (7), pp. 6378 |
|
dc.identifier.issn |
1661-6596 |
|
dc.identifier.issn |
1422-0067 |
|
dc.identifier.uri |
http://hdl.handle.net/10453/169920
|
|
dc.description.abstract |
<jats:p>Cardiovascular disease (CVD) is a leading cause of mortality worldwide, with cigarette smoking being a major preventable risk factor. Smoking cessation can be difficult due to the addictive nature of nicotine and the withdrawal symptoms following cessation. Electronic cigarettes (e-Cigs) have emerged as an alternative smoking cessation device, which has been increasingly used by non-smokers; however, the cardiovascular effects surrounding the use of e-Cigs remains unclear. This study aimed to investigate the effects of e-Cig aerosol condensate (EAC) (0 mg and 18 mg nicotine) in vitro on human coronary artery endothelial cells (HCAEC) and in vivo on the cardiovascular system using a mouse model of ‘e-vaping’. In vitro results show a decrease in cell viability of HCAEC when exposed to EAC either directly or after exposure to conditioned lung cell media (p < 0.05 vs. control). Reactive oxygen species were increased in HCAEC when exposed to EAC directly or after exposure to conditioned lung cell media (p < 0.0001 vs. control). ICAM-1 protein expression levels were increased after exposure to conditioned lung cell media (18 mg vs. control, p < 0.01). Ex vivo results show an increase in the mRNA levels of anti-angiogenic marker, FKBPL (p < 0.05 vs. sham), and endothelial cell adhesion molecule involved in barrier function, ICAM-1 (p < 0.05 vs. sham) in murine hearts following exposure to electronic cigarette aerosol treatment containing a higher amount of nicotine. Immunohistochemistry also revealed an upregulation of FKBPL and ICAM-1 protein expression levels. This study showed that despite e-Cigs being widely used for tobacco smoking cessation, these can negatively impact endothelial cell health with a potential to lead to the development of cardiovascular disease.</jats:p> |
|
dc.language |
en |
|
dc.publisher |
MDPI |
|
dc.relation |
http://purl.org/au-research/grants/nhmrc/1158186
|
|
dc.relation |
http://purl.org/au-research/grants/nhmrc/GNT1158186
|
|
dc.relation.ispartof |
International Journal of Molecular Sciences |
|
dc.relation.isbasedon |
10.3390/ijms24076378 |
|
dc.rights |
info:eu-repo/semantics/openAccess |
|
dc.subject |
0399 Other Chemical Sciences, 0604 Genetics, 0699 Other Biological Sciences |
|
dc.subject.classification |
Chemical Physics |
|
dc.title |
E-Cigarette Aerosol Condensate Leads to Impaired Coronary Endothelial Cell Health and Restricted Angiogenesis |
|
dc.type |
Journal Article |
|
utslib.citation.volume |
24 |
|
utslib.for |
0399 Other Chemical Sciences |
|
utslib.for |
0604 Genetics |
|
utslib.for |
0699 Other Biological Sciences |
|
pubs.organisational-group |
/University of Technology Sydney |
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Science |
|
pubs.organisational-group |
/University of Technology Sydney/Strength - CHT - Health Technologies |
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Science/School of Life Sciences |
|
pubs.organisational-group |
/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices |
|
pubs.organisational-group |
/University of Technology Sydney/Centre for Health Technologies (CHT) |
|
utslib.copyright.status |
open_access |
* |
dc.date.updated |
2023-04-18T03:47:49Z |
|
pubs.issue |
7 |
|
pubs.publication-status |
Published online |
|
pubs.volume |
24 |
|
utslib.citation.issue |
7 |
|