Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli.

Westlund, E; Bergenstråle, A; Pokhrel, A; Chan, H; Skoglund, U; Daley, DO; Söderström, B

Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli.

Westlund, E Bergenstråle, A Pokhrel, A Chan, H Skoglund, U Daley, DO Söderström, B

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Publisher:: Springer Nature
Publication Type:: Journal Article
Citation:: Curr Genet, 2023, 69, (2-3), pp. 153-163
Issue Date:: 2023-06

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Field	Value	Language
dc.contributor.author	Westlund, E
dc.contributor.author	Bergenstråle, A
dc.contributor.author	Pokhrel, A
dc.contributor.author	Chan, H
dc.contributor.author	Skoglund, U
dc.contributor.author	Daley, DO
dc.contributor.author	Söderström, B
dc.date.accessioned	2023-08-02T22:54:41Z
dc.date.available	2023-03-24
dc.date.available	2023-08-02T22:54:41Z
dc.date.issued	2023-06
dc.identifier.citation	Curr Genet, 2023, 69, (2-3), pp. 153-163
dc.identifier.issn	0172-8083
dc.identifier.issn	1432-0983
dc.identifier.uri	http://hdl.handle.net/10453/171703
dc.description.abstract	Understanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (ALFA), and a corresponding nanobody fused to mEos3.2. The methodology presented is applicable to other bacterial proteins.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Springer Nature
dc.relation	http://purl.org/au-research/grants/arc/DP220101143
dc.relation.ispartof	Curr Genet
dc.relation.isbasedon	10.1007/s00294-023-01266-2
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0604 Genetics, 0605 Microbiology
dc.subject.classification	Microbiology
dc.subject.classification	3105 Genetics
dc.subject.classification	3107 Microbiology
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Single-Domain Antibodies
dc.subject.mesh	Single Molecule Imaging
dc.subject.mesh	Cytoskeletal Proteins
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Escherichia coli Proteins
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Escherichia coli Proteins
dc.subject.mesh	Cytoskeletal Proteins
dc.subject.mesh	Single-Domain Antibodies
dc.subject.mesh	Single Molecule Imaging
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Single-Domain Antibodies
dc.subject.mesh	Single Molecule Imaging
dc.subject.mesh	Cytoskeletal Proteins
dc.subject.mesh	Bacterial Proteins
dc.subject.mesh	Escherichia coli Proteins
dc.title	Application of nanotags and nanobodies for live cell single-molecule imaging of the Z-ring in Escherichia coli.
dc.type	Journal Article
utslib.citation.volume	69
utslib.location.activity	United States
utslib.for	0604 Genetics
utslib.for	0605 Microbiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
utslib.copyright.status	closed_access	*
dc.date.updated	2023-08-02T22:54:32Z
pubs.issue	2-3
pubs.publication-status	Published
pubs.volume	69
utslib.citation.issue	2-3

Abstract:

Understanding where proteins are localized in a bacterial cell is essential for understanding their function and regulation. This is particularly important for proteins that are involved in cell division, which localize at the division septum and assemble into highly regulated complexes. Current knowledge of these complexes has been greatly facilitated by super-resolution imaging using fluorescent protein fusions. Herein, we demonstrate with FtsZ that single-molecule PALM images can be obtained in-vivo using a genetically fused nanotag (ALFA), and a corresponding nanobody fused to mEos3.2. The methodology presented is applicable to other bacterial proteins.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/171703