Probiotic Bifidobacterium longum subsp. longum Protects against Cigarette Smoke-Induced Inflammation in Mice.

Budden, KF; Gellatly, SL; Vaughan, A; Amorim, N; Horvat, JC; Hansbro, NG; Wood, DLA; Hugenholtz, P; Dennis, PG; Wark, PAB; Hansbro, PM

Probiotic Bifidobacterium longum subsp. longum Protects against Cigarette Smoke-Induced Inflammation in Mice.

Budden, KF Gellatly, SL Vaughan, A

Amorim, N Horvat, JC Hansbro, NG Wood, DLA Hugenholtz, P Dennis, PG Wark, PAB Hansbro, PM

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Int J Mol Sci, 2023, 24, (1), pp. 252
Issue Date:: 2023

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Field	Value	Language
dc.contributor.author	Budden, KF
dc.contributor.author	Gellatly, SL
dc.contributor.author	Vaughan, A https://orcid.org/0000-0001-5890-7877
dc.contributor.author	Amorim, N
dc.contributor.author	Horvat, JC
dc.contributor.author	Hansbro, NG
dc.contributor.author	Wood, DLA
dc.contributor.author	Hugenholtz, P
dc.contributor.author	Dennis, PG
dc.contributor.author	Wark, PAB
dc.contributor.author	Hansbro, PM
dc.date.accessioned	2023-08-09T04:58:54Z
dc.date.available	2022-12-20
dc.date.available	2023-08-09T04:58:54Z
dc.date.issued	2023
dc.identifier.citation	Int J Mol Sci, 2023, 24, (1), pp. 252
dc.identifier.issn	1422-0067
dc.identifier.issn	1422-0067
dc.identifier.uri	http://hdl.handle.net/10453/171757
dc.description.abstract	Bifidobacterium are prominent gut commensals that produce the short-chain fatty acid (SCFA) acetate, and they are often used as probiotics. Connections between the gut and the lung, termed the gut-lung axis, are regulated by the microbiome. The gut-lung axis is increasingly implicated in cigarette smoke-induced diseases, and cigarette smoke exposure has been associated with depletion of Bifidobacterium species. In this study, we assessed the impact of acetate-producing Bifidobacterium longum subsp. longum (WT) and a mutant strain with an impaired acetate production capacity (MUT) on cigarette smoke-induced inflammation. The mice were treated with WT or MUT B. longum subsp. longum and exposed to cigarette smoke for 8 weeks before assessments of lung inflammation, lung tissue gene expression and cecal SCFAs were performed. Both strains of B. longum subsp. longum reduced lung inflammation, inflammatory cytokine expression and adhesion factor expression and alleviated cigarette smoke-induced depletion in caecum butyrate. Thus, the probiotic administration of B. longum subsp. longum, irrespective of its acetate-producing capacity, alleviated cigarette smoke-induced inflammation and the depletion of cecal butyrate levels.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation	http://purl.org/au-research/grants/nhmrc/1175134
dc.relation	http://purl.org/au-research/grants/nhmrc/GNT1079187
dc.relation.ispartof	Int J Mol Sci
dc.relation.isbasedon	10.3390/ijms24010252
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0399 Other Chemical Sciences, 0604 Genetics, 0699 Other Biological Sciences
dc.subject.classification	Chemical Physics
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3107 Microbiology
dc.subject.classification	3404 Medicinal and biomolecular chemistry
dc.subject.mesh	Acetates
dc.subject.mesh	Animals
dc.subject.mesh	Bifidobacterium
dc.subject.mesh	Butyrates
dc.subject.mesh	Cigarette Smoking
dc.subject.mesh	Inflammation
dc.subject.mesh	Mice
dc.subject.mesh	Probiotics
dc.subject.mesh	Mice
dc.subject.mesh	Animals
dc.subject.mesh	Cigarette Smoking
dc.subject.mesh	Bifidobacterium
dc.subject.mesh	Probiotics
dc.subject.mesh	Butyrates
dc.subject.mesh	Acetates
dc.subject.mesh	Inflammation
dc.subject.mesh	Animals
dc.subject.mesh	Mice
dc.subject.mesh	Bifidobacterium
dc.subject.mesh	Inflammation
dc.subject.mesh	Acetates
dc.subject.mesh	Butyrates
dc.subject.mesh	Probiotics
dc.subject.mesh	Cigarette Smoking
dc.subject.mesh	Mice
dc.subject.mesh	Animals
dc.subject.mesh	Cigarette Smoking
dc.subject.mesh	Bifidobacterium
dc.subject.mesh	Probiotics
dc.subject.mesh	Butyrates
dc.subject.mesh	Acetates
dc.subject.mesh	Inflammation
dc.title	Probiotic Bifidobacterium longum subsp. longum Protects against Cigarette Smoke-Induced Inflammation in Mice.
dc.type	Journal Article
utslib.citation.volume	24
utslib.location.activity	Switzerland
utslib.for	0399 Other Chemical Sciences
utslib.for	0604 Genetics
utslib.for	0699 Other Biological Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFI - Centre for Inflammation
utslib.copyright.status	in_progress	*
pubs.consider-herdc	false
dc.date.updated	2023-08-09T04:58:49Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	24
utslib.citation.issue	1

Abstract:

Bifidobacterium are prominent gut commensals that produce the short-chain fatty acid (SCFA) acetate, and they are often used as probiotics. Connections between the gut and the lung, termed the gut-lung axis, are regulated by the microbiome. The gut-lung axis is increasingly implicated in cigarette smoke-induced diseases, and cigarette smoke exposure has been associated with depletion of Bifidobacterium species. In this study, we assessed the impact of acetate-producing Bifidobacterium longum subsp. longum (WT) and a mutant strain with an impaired acetate production capacity (MUT) on cigarette smoke-induced inflammation. The mice were treated with WT or MUT B. longum subsp. longum and exposed to cigarette smoke for 8 weeks before assessments of lung inflammation, lung tissue gene expression and cecal SCFAs were performed. Both strains of B. longum subsp. longum reduced lung inflammation, inflammatory cytokine expression and adhesion factor expression and alleviated cigarette smoke-induced depletion in caecum butyrate. Thus, the probiotic administration of B. longum subsp. longum, irrespective of its acetate-producing capacity, alleviated cigarette smoke-induced inflammation and the depletion of cecal butyrate levels.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/171757