Gene expression profiles in mesenchymal stromal cells from bone marrow, adipose tissue and lung tissue of COPD patients and controls.
Kruk, D
Yeung, ACY
Faiz, A
Ten Hacken, NHT
Timens, W
van Kuppevelt, TH
Daamen, W
Hof, D
Harmsen, MC
Rojas, M
Heijink, IH
- Publisher:
- Springer Nature
- Publication Type:
- Journal Article
- Citation:
- Respir Res, 2023, 24, (1), pp. 22
- Issue Date:
- 2023-01-21
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Kruk, D | |
dc.contributor.author | Yeung, ACY | |
dc.contributor.author |
Faiz, A https://orcid.org/0000-0003-1740-3538 |
|
dc.contributor.author | Ten Hacken, NHT | |
dc.contributor.author | Timens, W | |
dc.contributor.author | van Kuppevelt, TH | |
dc.contributor.author | Daamen, W | |
dc.contributor.author | Hof, D | |
dc.contributor.author | Harmsen, MC | |
dc.contributor.author | Rojas, M | |
dc.contributor.author | Heijink, IH | |
dc.date.accessioned | 2023-09-28T01:21:43Z | |
dc.date.available | 2023-01-03 | |
dc.date.available | 2023-09-28T01:21:43Z | |
dc.date.issued | 2023-01-21 | |
dc.identifier.citation | Respir Res, 2023, 24, (1), pp. 22 | |
dc.identifier.issn | 1465-9921 | |
dc.identifier.issn | 1465-993X | |
dc.identifier.uri | http://hdl.handle.net/10453/172359 | |
dc.description.abstract | BACKGROUND: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible lung tissue damage. Novel regenerative strategies are urgently awaited. Cultured mesenchymal stem/stromal cells (MSCs) have shown promising results in experimental models of COPD, but differences between sources may impact on their potential use in therapeutic strategies in patients. AIM: To assess the transcriptome of lung-derived MSCs (LMSCs), bone marrow-derived MSCs (BM-MSC) and adipose-derived MSCs (AD-MSCs) from COPD patients and non-COPD controls. METHODS: We studied differences in gene expression profiles between the MSC-subtypes, as well as between COPD and control using RNA sequencing (RNA-seq). RESULTS: We show that besides heterogeneity between donors, MSCs from different sources have strongly divergent gene signatures. The growth factors FGF10 and HGF were predominantly expressed in LMSCs. MSCs from all sources displayed altered expression profiles in COPD, with most pronounced significantly up- and downregulated genes in MSCs from adipose tissue. Pathway analysis revealed that the most differentially expressed genes in COPD-derived AD-MSCs are involved in extracellular matrix (ECM) binding and expression. In LMSCs, the gene that differed most strongly between COPD and control was CSGALNACT1, an ECM modulating gene. CONCLUSION: Autologous MSCs from COPD patients display abnormalities with respect to their transcriptome, which were surprisingly most profound in MSCs from extrapulmonary sources. LMSCs may be optimally equipped for lung tissue repair because of the expression of specific growth factor genes. | |
dc.format | Electronic | |
dc.language | eng | |
dc.publisher | Springer Nature | |
dc.relation.ispartof | Respir Res | |
dc.relation.isbasedon | 10.1186/s12931-023-02314-8 | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | 1102 Cardiorespiratory Medicine and Haematology, 1103 Clinical Sciences | |
dc.subject.classification | Respiratory System | |
dc.subject.classification | 3201 Cardiovascular medicine and haematology | |
dc.subject.classification | 3202 Clinical sciences | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Transcriptome | |
dc.subject.mesh | Bone Marrow | |
dc.subject.mesh | Adipose Tissue | |
dc.subject.mesh | Lung | |
dc.subject.mesh | Pulmonary Disease, Chronic Obstructive | |
dc.subject.mesh | Mesenchymal Stem Cells | |
dc.subject.mesh | Bone Marrow Cells | |
dc.subject.mesh | Cells, Cultured | |
dc.subject.mesh | Cell Differentiation | |
dc.subject.mesh | Lung | |
dc.subject.mesh | Adipose Tissue | |
dc.subject.mesh | Bone Marrow Cells | |
dc.subject.mesh | Cells, Cultured | |
dc.subject.mesh | Mesenchymal Stem Cells | |
dc.subject.mesh | Bone Marrow | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Pulmonary Disease, Chronic Obstructive | |
dc.subject.mesh | Cell Differentiation | |
dc.subject.mesh | Transcriptome | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Transcriptome | |
dc.subject.mesh | Bone Marrow | |
dc.subject.mesh | Adipose Tissue | |
dc.subject.mesh | Lung | |
dc.subject.mesh | Pulmonary Disease, Chronic Obstructive | |
dc.subject.mesh | Mesenchymal Stem Cells | |
dc.subject.mesh | Bone Marrow Cells | |
dc.subject.mesh | Cells, Cultured | |
dc.subject.mesh | Cell Differentiation | |
dc.title | Gene expression profiles in mesenchymal stromal cells from bone marrow, adipose tissue and lung tissue of COPD patients and controls. | |
dc.type | Journal Article | |
utslib.citation.volume | 24 | |
utslib.location.activity | England | |
utslib.for | 1102 Cardiorespiratory Medicine and Haematology | |
utslib.for | 1103 Clinical Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Life Sciences | |
pubs.organisational-group | /University of Technology Sydney/Centre for Health Technologies (CHT) | |
pubs.organisational-group | /University of Technology Sydney/Strength - CFI - Centre for Inflammation | |
utslib.copyright.status | open_access | * |
dc.date.updated | 2023-09-28T01:21:39Z | |
pubs.issue | 1 | |
pubs.publication-status | Published online | |
pubs.volume | 24 | |
utslib.citation.issue | 1 |
Abstract:
BACKGROUND: Chronic obstructive pulmonary disease (COPD) is characterized by irreversible lung tissue damage. Novel regenerative strategies are urgently awaited. Cultured mesenchymal stem/stromal cells (MSCs) have shown promising results in experimental models of COPD, but differences between sources may impact on their potential use in therapeutic strategies in patients. AIM: To assess the transcriptome of lung-derived MSCs (LMSCs), bone marrow-derived MSCs (BM-MSC) and adipose-derived MSCs (AD-MSCs) from COPD patients and non-COPD controls. METHODS: We studied differences in gene expression profiles between the MSC-subtypes, as well as between COPD and control using RNA sequencing (RNA-seq). RESULTS: We show that besides heterogeneity between donors, MSCs from different sources have strongly divergent gene signatures. The growth factors FGF10 and HGF were predominantly expressed in LMSCs. MSCs from all sources displayed altered expression profiles in COPD, with most pronounced significantly up- and downregulated genes in MSCs from adipose tissue. Pathway analysis revealed that the most differentially expressed genes in COPD-derived AD-MSCs are involved in extracellular matrix (ECM) binding and expression. In LMSCs, the gene that differed most strongly between COPD and control was CSGALNACT1, an ECM modulating gene. CONCLUSION: Autologous MSCs from COPD patients display abnormalities with respect to their transcriptome, which were surprisingly most profound in MSCs from extrapulmonary sources. LMSCs may be optimally equipped for lung tissue repair because of the expression of specific growth factor genes.
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