Integrative omics identifies conserved and pathogen-specific responses of sepsis-causing bacteria.

Mu, A; Klare, WP; Baines, SL; Ignatius Pang, CN; Guérillot, R; Harbison-Price, N; Keller, N; Wilksch, J; Nhu, NTK; Phan, M-D; Keller, B; Nijagal, B; Tull, D; Dayalan, S; Chua, HHC; Skoneczny, D; Koval, J; Hachani, A; Shah, AD; Neha, N; Jadhav, S; Partridge, SR; Cork, AJ; Peters, K; Bertolla, O; Brouwer, S; Hancock, SJ; Álvarez-Fraga, L; De Oliveira, DMP; Forde, B; Dale, A; Mujchariyakul, W; Walsh, CJ; Monk, I; Fitzgerald, A; Lum, M; Correa-Ospina, C; Roy Chowdhury, P; Parton, RG; De Voss, J; Beckett, J; Monty, F; McKinnon, J; Song, X; Stephen, JR; Everest, M; Bellgard, MI; Tinning, M; Leeming, M; Hocking, D; Jebeli, L; Wang, N; Ben Zakour, N; Yasar, SA; Vecchiarelli, S; Russell, T; Zaw, T; Chen, T; Teng, D; Kassir, Z; Lithgow, T; Jenney, A; Cole, JN; Nizet, V; Sorrell, TC; Peleg, AY; Paterson, DL; Beatson, SA; Wu, J; Molloy, MP; Syme, AE; Goode, RJA; Hunter, AA; Bowland, G; West, NP; Wilkins, MR; Djordjevic, SP; Davies, MR; Seemann, T; Howden, BP; Pascovici, D; Tyagi, S; Schittenhelm, RB; De Souza, DP; McConville, MJ; Iredell, JR; Cordwell, SJ; Strugnell, RA; Stinear, TP; Schembri, MA; Walker, MJ

Integrative omics identifies conserved and pathogen-specific responses of sepsis-causing bacteria.

Mu, A Klare, WP Baines, SL Ignatius Pang, CN Guérillot, R Harbison-Price, N Keller, N Wilksch, J Nhu, NTK Phan, M-D Keller, B Nijagal, B Tull, D Dayalan, S Chua, HHC Skoneczny, D Koval, J Hachani, A Shah, AD Neha, N Jadhav, S Partridge, SR Cork, AJ Peters, K Bertolla, O Brouwer, S Hancock, SJ Álvarez-Fraga, L De Oliveira, DMP Forde, B Dale, A Mujchariyakul, W Walsh, CJ Monk, I Fitzgerald, A Lum, M Correa-Ospina, C Roy Chowdhury, P Parton, RG De Voss, J Beckett, J Monty, F McKinnon, J Song, X Stephen, JR Everest, M Bellgard, MI Tinning, M Leeming, M Hocking, D Jebeli, L Wang, N Ben Zakour, N Yasar, SA Vecchiarelli, S Russell, T Zaw, T Chen, T Teng, D Kassir, Z Lithgow, T Jenney, A Cole, JN Nizet, V Sorrell, TC Peleg, AY Paterson, DL Beatson, SA Wu, J Molloy, MP Syme, AE Goode, RJA Hunter, AA Bowland, G West, NP Wilkins, MR Djordjevic, SP Davies, MR Seemann, T Howden, BP Pascovici, D Tyagi, S Schittenhelm, RB De Souza, DP McConville, MJ Iredell, JR Cordwell, SJ Strugnell, RA Stinear, TP Schembri, MA Walker, MJ

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Publisher:: Springer Nature
Publication Type:: Journal Article
Citation:: Nat Commun, 2023, 14, (1), pp. 1530
Issue Date:: 2023-03-18

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Field	Value	Language
dc.contributor.author	Mu, A
dc.contributor.author	Klare, WP
dc.contributor.author	Baines, SL
dc.contributor.author	Ignatius Pang, CN
dc.contributor.author	Guérillot, R
dc.contributor.author	Harbison-Price, N
dc.contributor.author	Keller, N
dc.contributor.author	Wilksch, J
dc.contributor.author	Nhu, NTK
dc.contributor.author	Phan, M-D
dc.contributor.author	Keller, B
dc.contributor.author	Nijagal, B
dc.contributor.author	Tull, D
dc.contributor.author	Dayalan, S
dc.contributor.author	Chua, HHC
dc.contributor.author	Skoneczny, D
dc.contributor.author	Koval, J
dc.contributor.author	Hachani, A
dc.contributor.author	Shah, AD
dc.contributor.author	Neha, N
dc.contributor.author	Jadhav, S
dc.contributor.author	Partridge, SR
dc.contributor.author	Cork, AJ
dc.contributor.author	Peters, K
dc.contributor.author	Bertolla, O
dc.contributor.author	Brouwer, S
dc.contributor.author	Hancock, SJ
dc.contributor.author	Álvarez-Fraga, L
dc.contributor.author	De Oliveira, DMP
dc.contributor.author	Forde, B
dc.contributor.author	Dale, A
dc.contributor.author	Mujchariyakul, W
dc.contributor.author	Walsh, CJ
dc.contributor.author	Monk, I
dc.contributor.author	Fitzgerald, A
dc.contributor.author	Lum, M
dc.contributor.author	Correa-Ospina, C
dc.contributor.author	Roy Chowdhury, P
dc.contributor.author	Parton, RG
dc.contributor.author	De Voss, J
dc.contributor.author	Beckett, J
dc.contributor.author	Monty, F
dc.contributor.author	McKinnon, J
dc.contributor.author	Song, X
dc.contributor.author	Stephen, JR
dc.contributor.author	Everest, M
dc.contributor.author	Bellgard, MI
dc.contributor.author	Tinning, M
dc.contributor.author	Leeming, M
dc.contributor.author	Hocking, D
dc.contributor.author	Jebeli, L
dc.contributor.author	Wang, N
dc.contributor.author	Ben Zakour, N
dc.contributor.author	Yasar, SA
dc.contributor.author	Vecchiarelli, S
dc.contributor.author	Russell, T
dc.contributor.author	Zaw, T
dc.contributor.author	Chen, T
dc.contributor.author	Teng, D
dc.contributor.author	Kassir, Z
dc.contributor.author	Lithgow, T
dc.contributor.author	Jenney, A
dc.contributor.author	Cole, JN
dc.contributor.author	Nizet, V
dc.contributor.author	Sorrell, TC
dc.contributor.author	Peleg, AY
dc.contributor.author	Paterson, DL
dc.contributor.author	Beatson, SA
dc.contributor.author	Wu, J
dc.contributor.author	Molloy, MP
dc.contributor.author	Syme, AE
dc.contributor.author	Goode, RJA
dc.contributor.author	Hunter, AA
dc.contributor.author	Bowland, G
dc.contributor.author	West, NP
dc.contributor.author	Wilkins, MR
dc.contributor.author	Djordjevic, SP
dc.contributor.author	Davies, MR
dc.contributor.author	Seemann, T
dc.contributor.author	Howden, BP
dc.contributor.author	Pascovici, D
dc.contributor.author	Tyagi, S
dc.contributor.author	Schittenhelm, RB
dc.contributor.author	De Souza, DP
dc.contributor.author	McConville, MJ
dc.contributor.author	Iredell, JR
dc.contributor.author	Cordwell, SJ
dc.contributor.author	Strugnell, RA
dc.contributor.author	Stinear, TP
dc.contributor.author	Schembri, MA
dc.contributor.author	Walker, MJ
dc.date.accessioned	2023-09-29T05:34:35Z
dc.date.available	2023-03-06
dc.date.available	2023-09-29T05:34:35Z
dc.date.issued	2023-03-18
dc.identifier.citation	Nat Commun, 2023, 14, (1), pp. 1530
dc.identifier.issn	2041-1723
dc.identifier.issn	2041-1723
dc.identifier.uri	http://hdl.handle.net/10453/172388
dc.description.abstract	Even in the setting of optimal resuscitation in high-income countries severe sepsis and septic shock have a mortality of 20-40%, with antibiotic resistance dramatically increasing this mortality risk. To develop a reference dataset enabling the identification of common bacterial targets for therapeutic intervention, we applied a standardized genomic, transcriptomic, proteomic and metabolomic technological framework to multiple clinical isolates of four sepsis-causing pathogens: Escherichia coli, Klebsiella pneumoniae species complex, Staphylococcus aureus and Streptococcus pyogenes. Exposure to human serum generated a sepsis molecular signature containing global increases in fatty acid and lipid biosynthesis and metabolism, consistent with cell envelope remodelling and nutrient adaptation for osmoprotection. In addition, acquisition of cholesterol was identified across the bacterial species. This detailed reference dataset has been established as an open resource to support discovery and translational research.
dc.format	Electronic
dc.language	eng
dc.publisher	Springer Nature
dc.relation.ispartof	Nat Commun
dc.relation.isbasedon	10.1038/s41467-023-37200-w
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Humans
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Proteomics
dc.subject.mesh	Sepsis
dc.subject.mesh	Bacteria
dc.subject.mesh	Staphylococcal Infections
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Klebsiella
dc.subject.mesh	Microbial Sensitivity Tests
dc.subject.mesh	Humans
dc.subject.mesh	Bacteria
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Klebsiella
dc.subject.mesh	Staphylococcal Infections
dc.subject.mesh	Sepsis
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Microbial Sensitivity Tests
dc.subject.mesh	Proteomics
dc.subject.mesh	Humans
dc.subject.mesh	Anti-Bacterial Agents
dc.subject.mesh	Proteomics
dc.subject.mesh	Sepsis
dc.subject.mesh	Bacteria
dc.subject.mesh	Staphylococcal Infections
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Klebsiella
dc.subject.mesh	Microbial Sensitivity Tests
dc.title	Integrative omics identifies conserved and pathogen-specific responses of sepsis-causing bacteria.
dc.type	Journal Article
utslib.citation.volume	14
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - AIMI - Australian Institute for Microbiology & Infection
utslib.copyright.status	open_access	*
dc.date.updated	2023-09-29T05:34:29Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	14
utslib.citation.issue	1

Abstract:

Even in the setting of optimal resuscitation in high-income countries severe sepsis and septic shock have a mortality of 20-40%, with antibiotic resistance dramatically increasing this mortality risk. To develop a reference dataset enabling the identification of common bacterial targets for therapeutic intervention, we applied a standardized genomic, transcriptomic, proteomic and metabolomic technological framework to multiple clinical isolates of four sepsis-causing pathogens: Escherichia coli, Klebsiella pneumoniae species complex, Staphylococcus aureus and Streptococcus pyogenes. Exposure to human serum generated a sepsis molecular signature containing global increases in fatty acid and lipid biosynthesis and metabolism, consistent with cell envelope remodelling and nutrient adaptation for osmoprotection. In addition, acquisition of cholesterol was identified across the bacterial species. This detailed reference dataset has been established as an open resource to support discovery and translational research.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/172388