Quantitative and qualitative assessment of DNA recovered from human skeletal remains
- Publisher:
- Elsevier
- Publication Type:
- Chapter
- Citation:
- Forensic Genetic Approaches for Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies, 2023, pp. 137-163
- Issue Date:
- 2023-01-01
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This chapter introduces DNA quantification and the importance of assessing the quantity and quality of DNA recovered from human skeletal remains to ensure optimal performance of downstream assays. Quantification methods and instruments commonly used by forensic laboratories are presented, with a focus on real-time quantitative PCR (qPCR) techniques. The suitability of each qPCR method for determining the amount of amplifiable total human, male, and/or mitochondrial DNA, and for detecting the presence of co-extracted inhibitors or degraded DNA, is discussed. Finally, explanations on how this quantitative and qualitative sample information should be utilized to guide selection of genetic markers, DNA input amount, and procedural modifications for addressing sample degradation and inhibition are provided.
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