Genotyping and sequencing of DNA recovered from human skeletal remains using capillary electrophoresis (CE)
- Publisher:
- Elsevier
- Publication Type:
- Chapter
- Citation:
- Forensic Genetic Approaches for Identification of Human Skeletal Remains: Challenges, Best Practices, and Emerging Technologies, 2023, pp. 285-323
- Issue Date:
- 2023-01-01
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Filename | Description | Size | |||
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21658649_12314205630005671.pdf | 3.01 MB |
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This chapter introduces capillary electrophoresis (CE) and how it is used for profiling DNA recovered from human skeletal remains. It will explain the most common fragment analysis and sequencing applications employed in forensic genetics, including the genotyping of autosomal short tandem repeats (STRs) and Sanger sequencing of mitochondrial DNA (mtDNA). The process of how PCR products are prepared, injected, separated, detected, and interpreted is discussed for each application. The CE platforms, chemistries, and analysis software commonly used by forensic laboratories are presented. Sample-specific CE artifacts often associated with skeletal samples are also detailed. Finally, the advantages and disadvantages of CE will be highlighted given the growing popularity of massively parallel sequencing (MPS).
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