Optimization of chromatographic buffer conditions for the simultaneous analysis of phosphatidylinositol and phosphatidylinositol phosphate species in canola.

Gertner, DS; Bishop, DP; Padula, MP

Optimization of chromatographic buffer conditions for the simultaneous analysis of phosphatidylinositol and phosphatidylinositol phosphate species in canola.

Gertner, DS Bishop, DP Padula, MP

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Publisher:: WILEY-V C H VERLAG GMBH
Publication Type:: Journal Article
Citation:: J Sep Sci, 2023, 46, (16), pp. e2300165
Issue Date:: 2023-08

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Field	Value	Language
dc.contributor.author	Gertner, DS
dc.contributor.author	Bishop, DP
dc.contributor.author	Padula, MP
dc.date.accessioned	2023-12-05T04:23:37Z
dc.date.available	2023-05-25
dc.date.available	2023-12-05T04:23:37Z
dc.date.issued	2023-08
dc.identifier.citation	J Sep Sci, 2023, 46, (16), pp. e2300165
dc.identifier.issn	1615-9306
dc.identifier.issn	1615-9314
dc.identifier.uri	http://hdl.handle.net/10453/173702
dc.description.abstract	The phosphatidylinositols and phosphatidylinositol phosphates are a set of closely related lipids known to influence various cellular functions. Irregular distributions of these molecules have been correlated with the development and progression of multiple diseases, including Alzheimer's, bipolar disorder, and various cancers. As a result, there is continued interest regarding the speciation of these compounds, with specific consideration on how their distribution may differ between healthy and diseased tissue. The comprehensive analysis of these compounds is challenging due to their varied and unique chemical characteristics, and current generalized lipidomics methods have proven unsuitable for phosphatidylinositol analysis and remain incapable of phosphatidylinositol phosphate analysis. Here we improved upon current methods by enabling the sensitive and simultaneous analysis of phosphatidylinositol and phosphatidylinositol phosphate species, whilst enhancing their characterization through chromatographic resolution between isomeric species. A 1 mM ammonium bicarbonate and ammonia buffer was determined optimal for this goal, enabling the identification of 148 phosphatidylinositide species, including 23 lyso-phosphatidylinositols, 51 phosphatidylinositols, 59 oxidized-phosphatidylinositols, and 15 phosphatidylinositol phosphates. As a result of this analysis, four distinct canola cultivars were differentiated based exclusively on their unique phosphatidylinositide-lipidome, indicating analyses of this type may be of use when considering the development and progression of the disease through lipidomic profiles.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY-V C H VERLAG GMBH
dc.relation.ispartof	J Sep Sci
dc.relation.isbasedon	10.1002/jssc.202300165
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0301 Analytical Chemistry, 0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Analytical Chemistry
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3401 Analytical chemistry
dc.subject.mesh	Phosphatidylinositols
dc.subject.mesh	Phosphatidylinositol Phosphates
dc.subject.mesh	Chromatography
dc.subject.mesh	Phosphates
dc.subject.mesh	Phosphates
dc.subject.mesh	Phosphatidylinositols
dc.subject.mesh	Phosphatidylinositol Phosphates
dc.subject.mesh	Chromatography
dc.subject.mesh	Phosphatidylinositols
dc.subject.mesh	Phosphatidylinositol Phosphates
dc.subject.mesh	Chromatography
dc.subject.mesh	Phosphates
dc.title	Optimization of chromatographic buffer conditions for the simultaneous analysis of phosphatidylinositol and phosphatidylinositol phosphate species in canola.
dc.type	Journal Article
utslib.citation.volume	46
utslib.location.activity	Germany
utslib.for	0301 Analytical Chemistry
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2023-12-05T04:23:35Z
pubs.issue	16
pubs.publication-status	Published
pubs.volume	46
utslib.citation.issue	16

Abstract:

The phosphatidylinositols and phosphatidylinositol phosphates are a set of closely related lipids known to influence various cellular functions. Irregular distributions of these molecules have been correlated with the development and progression of multiple diseases, including Alzheimer's, bipolar disorder, and various cancers. As a result, there is continued interest regarding the speciation of these compounds, with specific consideration on how their distribution may differ between healthy and diseased tissue. The comprehensive analysis of these compounds is challenging due to their varied and unique chemical characteristics, and current generalized lipidomics methods have proven unsuitable for phosphatidylinositol analysis and remain incapable of phosphatidylinositol phosphate analysis. Here we improved upon current methods by enabling the sensitive and simultaneous analysis of phosphatidylinositol and phosphatidylinositol phosphate species, whilst enhancing their characterization through chromatographic resolution between isomeric species. A 1 mM ammonium bicarbonate and ammonia buffer was determined optimal for this goal, enabling the identification of 148 phosphatidylinositide species, including 23 lyso-phosphatidylinositols, 51 phosphatidylinositols, 59 oxidized-phosphatidylinositols, and 15 phosphatidylinositol phosphates. As a result of this analysis, four distinct canola cultivars were differentiated based exclusively on their unique phosphatidylinositide-lipidome, indicating analyses of this type may be of use when considering the development and progression of the disease through lipidomic profiles.

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http://hdl.handle.net/10453/173702