Identification of a Novel LysR Family Transcriptional Regulator Controlling Acquisition of Sulfur Sources in Acinetobacter baumannii.

Pokhrel, A; Dinh, H; Li, L; Hassan, KA; Cain, AK; Paulsen, IT

Identification of a Novel LysR Family Transcriptional Regulator Controlling Acquisition of Sulfur Sources in Acinetobacter baumannii.

Pokhrel, A

Dinh, H Li, L Hassan, KA Cain, AK Paulsen, IT

Permalink

Publisher:: KARGER
Publication Type:: Journal Article
Citation:: Microb Physiol, 2023, 33, (1), pp. 27-35
Issue Date:: 2023

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (1.05 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Pokhrel, A https://orcid.org/0000-0001-8897-2397
dc.contributor.author	Dinh, H
dc.contributor.author	Li, L
dc.contributor.author	Hassan, KA
dc.contributor.author	Cain, AK
dc.contributor.author	Paulsen, IT
dc.date.accessioned	2024-01-11T04:02:43Z
dc.date.available	2022-12-30
dc.date.available	2024-01-11T04:02:43Z
dc.date.issued	2023
dc.identifier.citation	Microb Physiol, 2023, 33, (1), pp. 27-35
dc.identifier.issn	2673-1665
dc.identifier.issn	2673-1673
dc.identifier.uri	http://hdl.handle.net/10453/174304
dc.description.abstract	<sc>l</sc>-cysteine biosynthesis from inorganic sulfur represents a major mechanism by which reduced sulfur is incorporated into organic compounds. Cysteine biosynthesis and regulation is well characterized in Escherichia coli. However, the regulation of sulfur metabolism in Acinetobacter baumannii is only partly understood, with the LysR-type regulator, GigC known to control some aspects of sulfur reduction. In this study, we have used transcriptomics and bioinformatic analyses to characterize a novel LysR-type transcriptional regulator encoded by ABUW_1016 (cbl), in a highly multidrug resistant and virulent isolate of A. baumannii. We have shown that Cbl is involved in controlling expression of the genes required for uptake and reduction of various sulfur sources in A. baumannii. Collectively, we have identified the global regulon of Cbl and proposed a model of cysteine biosynthesis and its regulation by Cbl and GigC in A. baumannii.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	KARGER
dc.relation.ispartof	Microb Physiol
dc.relation.isbasedon	10.1159/000529038
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Acinetobacter baumannii
dc.subject.mesh	Cysteine
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Escherichia coli Proteins
dc.subject.mesh	Sulfur
dc.subject.mesh	Acinetobacter baumannii
dc.subject.mesh	Escherichia coli
dc.subject.mesh	Sulfur
dc.subject.mesh	Cysteine
dc.subject.mesh	Escherichia coli Proteins
dc.title	Identification of a Novel LysR Family Transcriptional Regulator Controlling Acquisition of Sulfur Sources in Acinetobacter baumannii.
dc.type	Journal Article
utslib.citation.volume	33
utslib.location.activity	Switzerland
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2024-01-11T04:02:42Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	33
utslib.citation.issue	1

Abstract:

l-cysteine biosynthesis from inorganic sulfur represents a major mechanism by which reduced sulfur is incorporated into organic compounds. Cysteine biosynthesis and regulation is well characterized in Escherichia coli. However, the regulation of sulfur metabolism in Acinetobacter baumannii is only partly understood, with the LysR-type regulator, GigC known to control some aspects of sulfur reduction. In this study, we have used transcriptomics and bioinformatic analyses to characterize a novel LysR-type transcriptional regulator encoded by ABUW_1016 (cbl), in a highly multidrug resistant and virulent isolate of A. baumannii. We have shown that Cbl is involved in controlling expression of the genes required for uptake and reduction of various sulfur sources in A. baumannii. Collectively, we have identified the global regulon of Cbl and proposed a model of cysteine biosynthesis and its regulation by Cbl and GigC in A. baumannii.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/174304