Exploring the potential of algae-bacteria interactions in the biocontrol of the marine pathogen Vibrio parahaemolyticus

Osborne, B; Siboni, N; Seymour, JR; Ralph, P; Pernice, M

Exploring the potential of algae-bacteria interactions in the biocontrol of the marine pathogen Vibrio parahaemolyticus

Osborne, B Siboni, N

Seymour, JR Ralph, P

Pernice, M

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Publisher:: SPRINGER
Publication Type:: Journal Article
Citation:: Journal of Applied Phycology, 2023, 35, (6), pp. 2731-2743
Issue Date:: 2023-12-01

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Field	Value	Language
dc.contributor.author	Osborne, B
dc.contributor.author	Siboni, N https://orcid.org/0000-0001-6082-0949
dc.contributor.author	Seymour, JR
dc.contributor.author	Ralph, P https://orcid.org/0000-0002-3103-7346
dc.contributor.author	Pernice, M https://orcid.org/0000-0002-3431-2104
dc.date.accessioned	2024-02-12T19:18:12Z
dc.date.available	2024-02-12T19:18:12Z
dc.date.issued	2023-12-01
dc.identifier.citation	Journal of Applied Phycology, 2023, 35, (6), pp. 2731-2743
dc.identifier.issn	0921-8971
dc.identifier.issn	1573-5176
dc.identifier.uri	http://hdl.handle.net/10453/175598
dc.description.abstract	The marine bacterium Vibrio parahaemolyticus is the leading cause of seafood-related food poisoning worldwide and a pathogen of marine species harvested in aquaculture. An outbreak of pathogenic V. parahaemolyticus within crustacean and bi-valve aquaculture facilities often results in significant loss of farmed product and in the spread of the pathogen into the human population. Evidence shows several marine bacteria and microalgae species have antibacterial effects against marine pathogens, including V. parahaemolyticus. This study explored the potential of combining possible mutualistic species as a consortium to enhance antibacterial properties against V. parahaemolyticus. Marine bacteria and microalgae were screened with the aim of identifying those able to successfully coexist while demonstrating growth suppression of multiple V. parahaemolyticus strains. This trial involved four screening phases to find effective inhibitor species and to gain insight into species-specific influences on cell growth. The combination of Tetraselmis sp. and Pseudoalteromonas peptidolytica achieved the greatest inhibition rate of V. parahaemolyticus of all combinations. This pairing resulted in the significant reduction of up to 24 ± 15% in Vibrio sp. copy number mL−1 day−1 in two of the five examined V. parahaemolyticus strains within five days, compared to control cultures absent of probiotic bacteria. Applying probiotic consortia such as this has the potential for use as a biocontrol technique within mariculture, but it will require additional research into the self-sustainability of successful consortia in natural aquatic conditions.
dc.language	English
dc.publisher	SPRINGER
dc.relation.ispartof	Journal of Applied Phycology
dc.relation.isbasedon	10.1007/s10811-023-03071-3
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0607 Plant Biology, 0704 Fisheries Sciences, 1002 Environmental Biotechnology
dc.subject.classification	Marine Biology & Hydrobiology
dc.subject.classification	3005 Fisheries sciences
dc.subject.classification	3108 Plant biology
dc.title	Exploring the potential of algae-bacteria interactions in the biocontrol of the marine pathogen Vibrio parahaemolyticus
dc.type	Journal Article
utslib.citation.volume	35
utslib.for	0607 Plant Biology
utslib.for	0704 Fisheries Sciences
utslib.for	1002 Environmental Biotechnology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	open_access	*
dc.date.updated	2024-02-12T19:18:11Z
pubs.issue	6
pubs.publication-status	Published
pubs.volume	35
utslib.citation.issue	6

Abstract:

The marine bacterium Vibrio parahaemolyticus is the leading cause of seafood-related food poisoning worldwide and a pathogen of marine species harvested in aquaculture. An outbreak of pathogenic V. parahaemolyticus within crustacean and bi-valve aquaculture facilities often results in significant loss of farmed product and in the spread of the pathogen into the human population. Evidence shows several marine bacteria and microalgae species have antibacterial effects against marine pathogens, including V. parahaemolyticus. This study explored the potential of combining possible mutualistic species as a consortium to enhance antibacterial properties against V. parahaemolyticus. Marine bacteria and microalgae were screened with the aim of identifying those able to successfully coexist while demonstrating growth suppression of multiple V. parahaemolyticus strains. This trial involved four screening phases to find effective inhibitor species and to gain insight into species-specific influences on cell growth. The combination of Tetraselmis sp. and Pseudoalteromonas peptidolytica achieved the greatest inhibition rate of V. parahaemolyticus of all combinations. This pairing resulted in the significant reduction of up to 24 ± 15% in Vibrio sp. copy number mL−1 day−1 in two of the five examined V. parahaemolyticus strains within five days, compared to control cultures absent of probiotic bacteria. Applying probiotic consortia such as this has the potential for use as a biocontrol technique within mariculture, but it will require additional research into the self-sustainability of successful consortia in natural aquatic conditions.

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http://hdl.handle.net/10453/175598