Characterization of the Flash-Induced Fluorescence Wave Phenomenon in the Coral Endosymbiont Algae, Symbiodiniaceae.

Mohammad Aslam, S; Vass, I; Szabó, M

Characterization of the Flash-Induced Fluorescence Wave Phenomenon in the Coral Endosymbiont Algae, Symbiodiniaceae.

Mohammad Aslam, S Vass, I Szabó, M

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Int J Mol Sci, 2023, 24, (10), pp. 8712
Issue Date:: 2023-05-13

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Field	Value	Language
dc.contributor.author	Mohammad Aslam, S
dc.contributor.author	Vass, I
dc.contributor.author	Szabó, M
dc.date.accessioned	2024-03-11T03:36:25Z
dc.date.available	2023-05-11
dc.date.available	2024-03-11T03:36:25Z
dc.date.issued	2023-05-13
dc.identifier.citation	Int J Mol Sci, 2023, 24, (10), pp. 8712
dc.identifier.issn	1422-0067
dc.identifier.issn	1422-0067
dc.identifier.uri	http://hdl.handle.net/10453/176456
dc.description.abstract	The dinoflagellate algae, Symbiodiniaceae, are significant symbiotic partners of corals due to their photosynthetic capacity. The photosynthetic processes of the microalgae consist of linear electron transport, which provides the energetic balance of ATP and NADPH production for CO2 fixation, and alternative electron transport pathways, including cyclic electron flow, which ensures the elevated ATP requirements under stress conditions. Flash-induced chlorophyll fluorescence relaxation is a non-invasive tool to assess the various electron transport pathways. A special case of fluorescence relaxation, the so-called wave phenomenon, was found to be associated with the activity of NAD(P)H dehydrogenase (NDH) in microalgae. We showed previously that the wave phenomenon existed in Symbiodiniaceae under acute heat stress and microaerobic conditions, however, the electron transport processes related to the wave phenomenon remained unknown. In this work, using various inhibitors, we show that (i) the linear electron transport has a crucial role in the formation of the wave, (ii) the inhibition of the donor side of Photosystem II did not induce the wave, whereas inhibition of the Calvin-Benson cycle accelerated it, (iii) the wave phenomenon was related to the operation of type II NDH (NDH-2). We therefore propose that the wave phenomenon is an important marker of the regulation of electron transport in Symbiodiniaceae.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation.ispartof	Int J Mol Sci
dc.relation.isbasedon	10.3390/ijms24108712
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0399 Other Chemical Sciences, 0604 Genetics, 0699 Other Biological Sciences
dc.subject.classification	Chemical Physics
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3107 Microbiology
dc.subject.classification	3404 Medicinal and biomolecular chemistry
dc.subject.mesh	Animals
dc.subject.mesh	Anthozoa
dc.subject.mesh	Fluorescence
dc.subject.mesh	Photosystem I Protein Complex
dc.subject.mesh	Photosynthesis
dc.subject.mesh	Electron Transport
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	Dinoflagellida
dc.subject.mesh	Adenosine Triphosphate
dc.subject.mesh	Chlorophyll
dc.subject.mesh	Animals
dc.subject.mesh	Anthozoa
dc.subject.mesh	Dinoflagellida
dc.subject.mesh	Chlorophyll
dc.subject.mesh	Photosystem I Protein Complex
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	Adenosine Triphosphate
dc.subject.mesh	Photosynthesis
dc.subject.mesh	Electron Transport
dc.subject.mesh	Fluorescence
dc.subject.mesh	Animals
dc.subject.mesh	Anthozoa
dc.subject.mesh	Fluorescence
dc.subject.mesh	Photosystem I Protein Complex
dc.subject.mesh	Photosynthesis
dc.subject.mesh	Electron Transport
dc.subject.mesh	Photosystem II Protein Complex
dc.subject.mesh	Dinoflagellida
dc.subject.mesh	Adenosine Triphosphate
dc.subject.mesh	Chlorophyll
dc.title	Characterization of the Flash-Induced Fluorescence Wave Phenomenon in the Coral Endosymbiont Algae, Symbiodiniaceae.
dc.type	Journal Article
utslib.citation.volume	24
utslib.location.activity	Switzerland
utslib.for	0399 Other Chemical Sciences
utslib.for	0604 Genetics
utslib.for	0699 Other Biological Sciences
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2024-03-11T03:36:23Z
pubs.issue	10
pubs.publication-status	Published online
pubs.volume	24
utslib.citation.issue	10

Abstract:

The dinoflagellate algae, Symbiodiniaceae, are significant symbiotic partners of corals due to their photosynthetic capacity. The photosynthetic processes of the microalgae consist of linear electron transport, which provides the energetic balance of ATP and NADPH production for CO2 fixation, and alternative electron transport pathways, including cyclic electron flow, which ensures the elevated ATP requirements under stress conditions. Flash-induced chlorophyll fluorescence relaxation is a non-invasive tool to assess the various electron transport pathways. A special case of fluorescence relaxation, the so-called wave phenomenon, was found to be associated with the activity of NAD(P)H dehydrogenase (NDH) in microalgae. We showed previously that the wave phenomenon existed in Symbiodiniaceae under acute heat stress and microaerobic conditions, however, the electron transport processes related to the wave phenomenon remained unknown. In this work, using various inhibitors, we show that (i) the linear electron transport has a crucial role in the formation of the wave, (ii) the inhibition of the donor side of Photosystem II did not induce the wave, whereas inhibition of the Calvin-Benson cycle accelerated it, (iii) the wave phenomenon was related to the operation of type II NDH (NDH-2). We therefore propose that the wave phenomenon is an important marker of the regulation of electron transport in Symbiodiniaceae.

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http://hdl.handle.net/10453/176456