Discovering an unknown territory using atom probe tomography: Elemental exchange at the bioceramic scaffold/bone tissue interface.

Holmes, NP; Roohani, I; Entezari, A; Guagliardo, P; Mirkhalaf, M; Lu, Z; Chen, Y-S; Yang, L; Dunstan, CR; Zreiqat, H; Cairney, JM

Discovering an unknown territory using atom probe tomography: Elemental exchange at the bioceramic scaffold/bone tissue interface.

Holmes, NP Roohani, I Entezari, A

Guagliardo, P Mirkhalaf, M Lu, Z Chen, Y-S Yang, L

Dunstan, CR Zreiqat, H Cairney, JM

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Publisher:: ELSEVIER SCI LTD
Publication Type:: Journal Article
Citation:: Acta Biomater, 2023, 162, pp. 199-210
Issue Date:: 2023-05

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Field	Value	Language
dc.contributor.author	Holmes, NP
dc.contributor.author	Roohani, I
dc.contributor.author	Entezari, A https://orcid.org/0000-0003-0504-8364
dc.contributor.author	Guagliardo, P
dc.contributor.author	Mirkhalaf, M
dc.contributor.author	Lu, Z
dc.contributor.author	Chen, Y-S
dc.contributor.author	Yang, L https://orcid.org/0000-0003-0123-1540
dc.contributor.author	Dunstan, CR
dc.contributor.author	Zreiqat, H
dc.contributor.author	Cairney, JM
dc.date.accessioned	2024-03-20T02:09:22Z
dc.date.available	2023-02-28
dc.date.available	2024-03-20T02:09:22Z
dc.date.issued	2023-05
dc.identifier.citation	Acta Biomater, 2023, 162, pp. 199-210
dc.identifier.issn	1742-7061
dc.identifier.issn	1878-7568
dc.identifier.uri	http://hdl.handle.net/10453/176942
dc.description.abstract	Here we report the first atom probe study to reveal the atomic-scale composition of in vivo bone formed in a bioceramic scaffold (strontium-hardystonite-gahnite) after 12-month implantation in a large bone defect in sheep tibia. The composition of the newly formed bone tissue differs to that of mature cortical bone tissue, and elements from the degrading bioceramic implant, particularly aluminium (Al), are present in both the newly formed bone and in the original mature cortical bone tissue at the perimeter of the bioceramic implant. Atom probe tomography confirmed that the trace elements are released from the bioceramic and are actively transported into the newly formed bone. NanoSIMS mapping, as a complementary technique, confirmed the distribution of the released ions from the bioceramic into the newly formed bone tissue within the scaffold. This study demonstrated the combined benefits of atom probe and nanoSIMS in assessing nanoscopic chemical composition changes at precise locations within the tissue/biomaterial interface. Such information can assist in understanding the interaction of scaffolds with surrounding tissue, hence permitting further iterative improvements to the design and performance of biomedical implants, and ultimately reducing the risk of complications or failure while increasing the rate of tissue formation. STATEMENT OF SIGNIFICANCE: The repair of critical-sized load-bearing bone defects is a challenge, and precisely engineered bioceramic scaffold implants is an emerging potential treatment strategy. However, we still do not understand the effect of the bioceramic scaffold implants on the composition of newly formed bone in vivo and surrounding existing mature bone. This article reports an innovative route to solve this problem, the combined power of atom probe tomography and nanoSIMS is used to spatially define elemental distributions across bioceramic implant sites. We determine the nanoscopic chemical composition changes at the Sr-HT Gahnite bioceramic/bone tissue interface, and importantly, provide the first report of in vivo bone tissue chemical composition formed in a bioceramic scaffold.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ELSEVIER SCI LTD
dc.relation.ispartof	Acta Biomater
dc.relation.isbasedon	10.1016/j.actbio.2023.02.039
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Biomedical Engineering
dc.subject.mesh	Animals
dc.subject.mesh	Sheep
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Biocompatible Materials
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Bone and Bones
dc.subject.mesh	Tomography
dc.subject.mesh	Bone and Bones
dc.subject.mesh	Animals
dc.subject.mesh	Sheep
dc.subject.mesh	Biocompatible Materials
dc.subject.mesh	Tomography
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Animals
dc.subject.mesh	Sheep
dc.subject.mesh	Tissue Scaffolds
dc.subject.mesh	Biocompatible Materials
dc.subject.mesh	Osteogenesis
dc.subject.mesh	Bone and Bones
dc.subject.mesh	Tomography
dc.title	Discovering an unknown territory using atom probe tomography: Elemental exchange at the bioceramic scaffold/bone tissue interface.
dc.type	Journal Article
utslib.citation.volume	162
utslib.location.activity	England
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	University of Technology Sydney/Strength - CGT - Centre for Green Technology
utslib.copyright.status	closed_access	*
dc.date.updated	2024-03-20T02:09:19Z
pubs.publication-status	Published
pubs.volume	162

Abstract:

Here we report the first atom probe study to reveal the atomic-scale composition of in vivo bone formed in a bioceramic scaffold (strontium-hardystonite-gahnite) after 12-month implantation in a large bone defect in sheep tibia. The composition of the newly formed bone tissue differs to that of mature cortical bone tissue, and elements from the degrading bioceramic implant, particularly aluminium (Al), are present in both the newly formed bone and in the original mature cortical bone tissue at the perimeter of the bioceramic implant. Atom probe tomography confirmed that the trace elements are released from the bioceramic and are actively transported into the newly formed bone. NanoSIMS mapping, as a complementary technique, confirmed the distribution of the released ions from the bioceramic into the newly formed bone tissue within the scaffold. This study demonstrated the combined benefits of atom probe and nanoSIMS in assessing nanoscopic chemical composition changes at precise locations within the tissue/biomaterial interface. Such information can assist in understanding the interaction of scaffolds with surrounding tissue, hence permitting further iterative improvements to the design and performance of biomedical implants, and ultimately reducing the risk of complications or failure while increasing the rate of tissue formation. STATEMENT OF SIGNIFICANCE: The repair of critical-sized load-bearing bone defects is a challenge, and precisely engineered bioceramic scaffold implants is an emerging potential treatment strategy. However, we still do not understand the effect of the bioceramic scaffold implants on the composition of newly formed bone in vivo and surrounding existing mature bone. This article reports an innovative route to solve this problem, the combined power of atom probe tomography and nanoSIMS is used to spatially define elemental distributions across bioceramic implant sites. We determine the nanoscopic chemical composition changes at the Sr-HT Gahnite bioceramic/bone tissue interface, and importantly, provide the first report of in vivo bone tissue chemical composition formed in a bioceramic scaffold.

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http://hdl.handle.net/10453/176942