Multi-Chemical Omics Analysis of the Symbiodiniaceae Durusdinium trenchii under Heat Stress.

Matthews, JL; Ueland, M; Bartels, N; Lawson, CA; Lockwood, TE; Wu, Y; Camp, EF

Multi-Chemical Omics Analysis of the Symbiodiniaceae Durusdinium trenchii under Heat Stress.

Matthews, JL Ueland, M

Bartels, N Lawson, CA Lockwood, TE Wu, Y Camp, EF

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Microorganisms, 2024, 12, (2), pp. 317
Issue Date:: 2024-02-02

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Field	Value	Language
dc.contributor.author	Matthews, JL
dc.contributor.author	Ueland, M https://orcid.org/0000-0002-9155-3502
dc.contributor.author	Bartels, N
dc.contributor.author	Lawson, CA
dc.contributor.author	Lockwood, TE
dc.contributor.author	Wu, Y
dc.contributor.author	Camp, EF
dc.date.accessioned	2024-08-02T02:40:55Z
dc.date.available	2024-01-24
dc.date.available	2024-08-02T02:40:55Z
dc.date.issued	2024-02-02
dc.identifier.citation	Microorganisms, 2024, 12, (2), pp. 317
dc.identifier.issn	2076-2607
dc.identifier.issn	2076-2607
dc.identifier.uri	http://hdl.handle.net/10453/179993
dc.description.abstract	The urgency of responding to climate change for corals necessitates the exploration of innovative methods to swiftly enhance our understanding of crucial processes. In this study, we employ an integrated chemical omics approach, combining elementomics, metabolomics, and volatilomics methodologies to unravel the biochemical pathways associated with the thermal response of the coral symbiont, Symbiodiniaceae Durusdinium trenchii. We outline the complimentary sampling approaches and discuss the standardised data corrections used to allow data integration and comparability. Our findings highlight the efficacy of individual methods in discerning differences in the biochemical response of D. trenchii under both control and stress-inducing temperatures. However, a deeper insight emerges when these methods are integrated, offering a more comprehensive understanding, particularly regarding oxidative stress pathways. Employing correlation network analysis enhanced the interpretation of volatile data, shedding light on the potential metabolic origins of volatiles with undescribed functions and presenting promising candidates for further exploration. Elementomics proves to be less straightforward to integrate, likely due to no net change in elements but rather elements being repurposed across compounds. The independent and integrated data from this study informs future omic profiling studies and recommends candidates for targeted research beyond Symbiodiniaceae biology. This study highlights the pivotal role of omic integration in advancing our knowledge, addressing critical gaps, and guiding future research directions in the context of climate change and coral reef preservation.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation	http://purl.org/au-research/grants/arc/DE210100494
dc.relation.ispartof	Microorganisms
dc.relation.isbasedon	10.3390/microorganisms12020317
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	3107 Microbiology
dc.subject.classification	3207 Medical microbiology
dc.title	Multi-Chemical Omics Analysis of the Symbiodiniaceae Durusdinium trenchii under Heat Stress.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	Switzerland
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	University of Technology Sydney/All Manual Groups
pubs.organisational-group	University of Technology Sydney/All Manual Groups/Climate Change Cluster Research Strength (C3)
pubs.organisational-group	University of Technology Sydney/All Manual Groups/Centre for Forensic Science (CFS)
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2024-08-02T02:40:47Z
pubs.issue	2
pubs.publication-status	Published online
pubs.volume	12
utslib.citation.issue	2

Abstract:

The urgency of responding to climate change for corals necessitates the exploration of innovative methods to swiftly enhance our understanding of crucial processes. In this study, we employ an integrated chemical omics approach, combining elementomics, metabolomics, and volatilomics methodologies to unravel the biochemical pathways associated with the thermal response of the coral symbiont, Symbiodiniaceae Durusdinium trenchii. We outline the complimentary sampling approaches and discuss the standardised data corrections used to allow data integration and comparability. Our findings highlight the efficacy of individual methods in discerning differences in the biochemical response of D. trenchii under both control and stress-inducing temperatures. However, a deeper insight emerges when these methods are integrated, offering a more comprehensive understanding, particularly regarding oxidative stress pathways. Employing correlation network analysis enhanced the interpretation of volatile data, shedding light on the potential metabolic origins of volatiles with undescribed functions and presenting promising candidates for further exploration. Elementomics proves to be less straightforward to integrate, likely due to no net change in elements but rather elements being repurposed across compounds. The independent and integrated data from this study informs future omic profiling studies and recommends candidates for targeted research beyond Symbiodiniaceae biology. This study highlights the pivotal role of omic integration in advancing our knowledge, addressing critical gaps, and guiding future research directions in the context of climate change and coral reef preservation.

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http://hdl.handle.net/10453/179993