A dynamic WUSCHEL/Layer 1 interplay directs shoot apical meristem formation during regeneration in tobacco.

Kumar, M; Ayzenshtat, D; Rather, GA; Zemach, H; Belausov, E; Eshed Williams, L; Bocobza, S

A dynamic WUSCHEL/Layer 1 interplay directs shoot apical meristem formation during regeneration in tobacco.

Kumar, M

Ayzenshtat, D Rather, GA Zemach, H Belausov, E Eshed Williams, L Bocobza, S

Permalink

Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Plant J, 2024, 120, (2), pp. 578-597
Issue Date:: 2024-10

Closed Access

	Filename	Description	Size
	The Plant Journal - 2024 - Kumar - A dynamic WUSCHEL Layer 1 interplay directs shoot apical meristem formation during.pdf	Published version	21.08 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Kumar, M https://orcid.org/0000-0002-5247-3059
dc.contributor.author	Ayzenshtat, D
dc.contributor.author	Rather, GA
dc.contributor.author	Zemach, H
dc.contributor.author	Belausov, E
dc.contributor.author	Eshed Williams, L
dc.contributor.author	Bocobza, S
dc.date.accessioned	2024-11-04T03:16:43Z
dc.date.available	2024-08-14
dc.date.available	2024-11-04T03:16:43Z
dc.date.issued	2024-10
dc.identifier.citation	Plant J, 2024, 120, (2), pp. 578-597
dc.identifier.issn	0960-7412
dc.identifier.issn	1365-313X
dc.identifier.uri	http://hdl.handle.net/10453/181698
dc.description.abstract	De novo shoot apical meristem (SAM) organogenesis during regeneration in tissue culture has been investigated for several decades, but the precise mechanisms governing early-stage cell fate specification remain elusive. In contrast to SAM establishment during embryogenesis, in vitro SAM formation occurs without positional cues and is characterized by autonomous initiation of cellular patterning. Here, we report on the initial stages of SAM organogenesis and on the molecular mechanisms that orchestrate gene patterning to establish SAM homeostasis. We found that SAM organogenesis in tobacco calli starts with protuberance formation followed by the formation of an intact L1 layer covering the nascent protuberance. We also exposed a complex interdependent relationship between L1 and WUS expression and revealed that any disruption in this interplay compromises shoot formation. Silencing WUS in nascent protuberances prevented L1 formation and caused the disorganization of the outer cell layers exhibiting both anticlinal and periclinal divisions, suggesting WUS plays a critical role in the proper establishment and organization of L1 during SAM organogenesis. We further discovered that silencing TONNEAU1 prevents the exclusive occurrence of anticlinal divisions in the outermost layer of the protuberances and suppresses the acquisition of L1 cellular identity and L1 formation, ultimately impeding SAM formation and regeneration. This study provides a novel molecular framework for the characterization of a WUS/L1 interplay that mediates SAM formation during regeneration.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation.ispartof	Plant J
dc.relation.isbasedon	10.1111/tpj.17002
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0601 Biochemistry and Cell Biology, 0607 Plant Biology
dc.subject.classification	Plant Biology & Botany
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3108 Plant biology
dc.subject.mesh	Meristem
dc.subject.mesh	Nicotiana
dc.subject.mesh	Plant Proteins
dc.subject.mesh	Gene Expression Regulation, Plant
dc.subject.mesh	Regeneration
dc.subject.mesh	Plant Shoots
dc.subject.mesh	Organogenesis, Plant
dc.subject.mesh	Homeodomain Proteins
dc.subject.mesh	Plant Shoots
dc.subject.mesh	Meristem
dc.subject.mesh	Homeodomain Proteins
dc.subject.mesh	Plant Proteins
dc.subject.mesh	Regeneration
dc.subject.mesh	Gene Expression Regulation, Plant
dc.subject.mesh	Organogenesis, Plant
dc.subject.mesh	Nicotiana
dc.title	A dynamic WUSCHEL/Layer 1 interplay directs shoot apical meristem formation during regeneration in tobacco.
dc.type	Journal Article
utslib.citation.volume	120
utslib.location.activity	England
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	0607 Plant Biology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Climate Change Cluster Research Strength (C3)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Climate Change Cluster Research Strength (C3)/Associate Member
utslib.copyright.status	closed_access	*
dc.date.updated	2024-11-04T03:16:39Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	120
utslib.citation.issue	2

Abstract:

De novo shoot apical meristem (SAM) organogenesis during regeneration in tissue culture has been investigated for several decades, but the precise mechanisms governing early-stage cell fate specification remain elusive. In contrast to SAM establishment during embryogenesis, in vitro SAM formation occurs without positional cues and is characterized by autonomous initiation of cellular patterning. Here, we report on the initial stages of SAM organogenesis and on the molecular mechanisms that orchestrate gene patterning to establish SAM homeostasis. We found that SAM organogenesis in tobacco calli starts with protuberance formation followed by the formation of an intact L1 layer covering the nascent protuberance. We also exposed a complex interdependent relationship between L1 and WUS expression and revealed that any disruption in this interplay compromises shoot formation. Silencing WUS in nascent protuberances prevented L1 formation and caused the disorganization of the outer cell layers exhibiting both anticlinal and periclinal divisions, suggesting WUS plays a critical role in the proper establishment and organization of L1 during SAM organogenesis. We further discovered that silencing TONNEAU1 prevents the exclusive occurrence of anticlinal divisions in the outermost layer of the protuberances and suppresses the acquisition of L1 cellular identity and L1 formation, ultimately impeding SAM formation and regeneration. This study provides a novel molecular framework for the characterization of a WUS/L1 interplay that mediates SAM formation during regeneration.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/181698