Extracellular vesicle cargo : a source of biomarkers for severe malaria in paediatric patients

Cheng, Iris Simone

Extracellular vesicle cargo : a source of biomarkers for severe malaria in paediatric patients

Cheng, Iris Simone

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Publication Type:: Thesis
Issue Date:: 2024

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Field	Value	Language
dc.contributor.author	Cheng, Iris Simone
dc.date.accessioned	2025-01-13T01:36:55Z
dc.date.available	2025-01-13T01:36:55Z
dc.date.issued	2024
dc.identifier.uri	http://hdl.handle.net/10453/183301
dc.description	University of Technology Sydney. Faculty of Science.	en_US.UTF-8
dc.description.abstract	For decades, malaria has remained a global health concern, if allowed to progress, it can develop into severe malaria and is a leading cause of death among children under five years old in Sub-Saharan Africa. Current treatments can eliminate malaria infection; however, the long-lasting effects, such as learning and motor difficulties, are more problematic to treat post-infection. The need for prognostic biomarkers of severe malaria is essential, as this would allow for earlier treatment and adjunctive therapy application, decreasing the lasting effects of symptomatic malaria. This thesis uses molecular and high-throughput -omics to investigate potential biomarkers carried by extracellular vesicles (EVs) from the plasma of adults and children who developed severe malaria, respectively. In addition to plasma-derived EVs, cerebrospinal fluid-derived EVs from children with severe malaria were also phenotypically analysed using highly sensitive cytometric techniques. This allowed the identification of miRNAs and proteins with high prognostic value when differentiating the outcomes of severe malaria, as well as the identification of the EV's phenotypic cellular origin. Throughout the findings, miRNA, protein and phenotypic differences were evident for children with neurological complications, indicating the importance of prognostic biomarkers for the identification of children with neurological complications.	en_US.UTF-8
dc.format	Thesis (PhD)
dc.language.iso	en_US	en_US.UTF-8
dc.relation	https://opus.lib.uts.edu.au/bitstream/10453/183301/1/thesis.pdf
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	The author owns the copyright in this thesis including all reproduction and reuse rights for the work. The work may not be altered without the permission of the copyright owner. Attribution is essential when quoting or paraphrasing from this thesis.
dc.rights	© 2024 Iris Simone Cheng
dc.rights	au.edu.uts.lib/cph
dc.title	Extracellular vesicle cargo : a source of biomarkers for severe malaria in paediatric patients	en_US.UTF-8
dc.type	Thesis
utslib.copyright.status	open_access	*

Abstract:

For decades, malaria has remained a global health concern, if allowed to progress, it can develop into severe malaria and is a leading cause of death among children under five years old in Sub-Saharan Africa. Current treatments can eliminate malaria infection; however, the long-lasting effects, such as learning and motor difficulties, are more problematic to treat post-infection. The need for prognostic biomarkers of severe malaria is essential, as this would allow for earlier treatment and adjunctive therapy application, decreasing the lasting effects of symptomatic malaria. This thesis uses molecular and high-throughput -omics to investigate potential biomarkers carried by extracellular vesicles (EVs) from the plasma of adults and children who developed severe malaria, respectively. In addition to plasma-derived EVs, cerebrospinal fluid-derived EVs from children with severe malaria were also phenotypically analysed using highly sensitive cytometric techniques. This allowed the identification of miRNAs and proteins with high prognostic value when differentiating the outcomes of severe malaria, as well as the identification of the EV's phenotypic cellular origin. Throughout the findings, miRNA, protein and phenotypic differences were evident for children with neurological complications, indicating the importance of prognostic biomarkers for the identification of children with neurological complications.

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http://hdl.handle.net/10453/183301