Mpeg1 is not essential for antibacterial or antiviral immunity, but is implicated in antigen presentation.

Ebrahimnezhaddarzi, S; Bird, CH; Allison, CC; Tuipulotu, DE; Kostoulias, X; Macri, C; Stutz, MD; Abraham, G; Kaiserman, D; Pang, SS; Man, SM; Mintern, JD; Naderer, T; Peleg, AY; Pellegrini, M; Whisstock, JC; Bird, PI

Mpeg1 is not essential for antibacterial or antiviral immunity, but is implicated in antigen presentation.

Ebrahimnezhaddarzi, S Bird, CH Allison, CC Tuipulotu, DE Kostoulias, X Macri, C Stutz, MD Abraham, G Kaiserman, D Pang, SS Man, SM Mintern, JD Naderer, T Peleg, AY Pellegrini, M Whisstock, JC Bird, PI

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Immunol Cell Biol, 2022, 100, (7), pp. 529-546
Issue Date:: 2022-08

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Field	Value	Language
dc.contributor.author	Ebrahimnezhaddarzi, S
dc.contributor.author	Bird, CH
dc.contributor.author	Allison, CC
dc.contributor.author	Tuipulotu, DE
dc.contributor.author	Kostoulias, X
dc.contributor.author	Macri, C
dc.contributor.author	Stutz, MD
dc.contributor.author	Abraham, G
dc.contributor.author	Kaiserman, D
dc.contributor.author	Pang, SS
dc.contributor.author	Man, SM
dc.contributor.author	Mintern, JD
dc.contributor.author	Naderer, T
dc.contributor.author	Peleg, AY
dc.contributor.author	Pellegrini, M
dc.contributor.author	Whisstock, JC
dc.contributor.author	Bird, PI
dc.date.accessioned	2025-01-15T13:30:28Z
dc.date.available	2022-04-25
dc.date.available	2025-01-15T13:30:28Z
dc.date.issued	2022-08
dc.identifier.citation	Immunol Cell Biol, 2022, 100, (7), pp. 529-546
dc.identifier.issn	0818-9641
dc.identifier.issn	1440-1711
dc.identifier.uri	http://hdl.handle.net/10453/183652
dc.description.abstract	To control infections phagocytes can directly kill invading microbes. Macrophage-expressed gene 1 (Mpeg1), a pore-forming protein sometimes known as perforin-2, is reported to be essential for bacterial killing following phagocytosis. Mice homozygous for the mutant allele Mpeg1tm1Pod succumb to bacterial infection and exhibit deficiencies in bacterial killing in vitro. Here we describe a new Mpeg mutant allele Mpeg1tm1.1Pib on the C57BL/6J background. Mice homozygous for the new allele are not abnormally susceptible to bacterial or viral infection, and irrespective of genetic background show no perturbation in bacterial killing in vitro. Potential reasons for these conflicting findings are discussed. In further work, we show that cytokine responses to inflammatory mediators, as well as antibody generation, are also normal in Mpeg1tm1.1Pib/tm1.1Pib mice. We also show that Mpeg1 is localized to a CD68-positive endolysosomal compartment, and that it exists predominantly as a processed, two-chain disulfide-linked molecule. It is abundant in conventional dendritic cells 1, and mice lacking Mpeg1 do not present the model antigen ovalbumin efficiently. We conclude that Mpeg1 is not essential for innate antibacterial protection or antiviral immunity, but may play a focused role early in the adaptive immune response.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation.ispartof	Immunol Cell Biol
dc.relation.isbasedon	10.1111/imcb.12554
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology, 1107 Immunology
dc.subject.classification	Immunology
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.classification	3204 Immunology
dc.subject.mesh	Animals
dc.subject.mesh	Antigen Presentation
dc.subject.mesh	Bacterial Infections
dc.subject.mesh	Immunity, Innate
dc.subject.mesh	Mice
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Pore Forming Cytotoxic Proteins
dc.subject.mesh	Virus Diseases
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Mice
dc.subject.mesh	Bacterial Infections
dc.subject.mesh	Virus Diseases
dc.subject.mesh	Antigen Presentation
dc.subject.mesh	Pore Forming Cytotoxic Proteins
dc.subject.mesh	Immunity, Innate
dc.subject.mesh	Animals
dc.subject.mesh	Antigen Presentation
dc.subject.mesh	Bacterial Infections
dc.subject.mesh	Immunity, Innate
dc.subject.mesh	Mice
dc.subject.mesh	Mice, Inbred C57BL
dc.subject.mesh	Pore Forming Cytotoxic Proteins
dc.subject.mesh	Virus Diseases
dc.title	Mpeg1 is not essential for antibacterial or antiviral immunity, but is implicated in antigen presentation.
dc.type	Journal Article
utslib.citation.volume	100
utslib.location.activity	United States
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	1107 Immunology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated	2025-01-15T13:30:25Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	100
utslib.citation.issue	7

Abstract:

To control infections phagocytes can directly kill invading microbes. Macrophage-expressed gene 1 (Mpeg1), a pore-forming protein sometimes known as perforin-2, is reported to be essential for bacterial killing following phagocytosis. Mice homozygous for the mutant allele Mpeg1tm1Pod succumb to bacterial infection and exhibit deficiencies in bacterial killing in vitro. Here we describe a new Mpeg mutant allele Mpeg1tm1.1Pib on the C57BL/6J background. Mice homozygous for the new allele are not abnormally susceptible to bacterial or viral infection, and irrespective of genetic background show no perturbation in bacterial killing in vitro. Potential reasons for these conflicting findings are discussed. In further work, we show that cytokine responses to inflammatory mediators, as well as antibody generation, are also normal in Mpeg1tm1.1Pib/tm1.1Pib mice. We also show that Mpeg1 is localized to a CD68-positive endolysosomal compartment, and that it exists predominantly as a processed, two-chain disulfide-linked molecule. It is abundant in conventional dendritic cells 1, and mice lacking Mpeg1 do not present the model antigen ovalbumin efficiently. We conclude that Mpeg1 is not essential for innate antibacterial protection or antiviral immunity, but may play a focused role early in the adaptive immune response.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/183652