Chlorella-enriched hydrogels protect against myocardial damage and reactive oxygen species production in anin vitroischemia/reperfusion model using cardiac spheroids.

Tarsitano, M; Liu Chung Ming, C; Bennar, L; Mahmodi, H; Wyllie, K; Idais, D; Al Shamery, W; Paolino, D; Cox, TR; Kabakova, I; Ralph, P; Gentile, C

Chlorella-enriched hydrogels protect against myocardial damage and reactive oxygen species production in anin vitroischemia/reperfusion model using cardiac spheroids.

Tarsitano, M Liu Chung Ming, C Bennar, L Mahmodi, H Wyllie, K Idais, D Al Shamery, W Paolino, D Cox, TR Kabakova, I

Ralph, P

Gentile, C

Permalink

Publisher:: IOP Publishing Ltd
Publication Type:: Journal Article
Citation:: Biofabrication, 2024, 17, (1)
Issue Date:: 2024-10-24

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (2.98 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Tarsitano, M
dc.contributor.author	Liu Chung Ming, C
dc.contributor.author	Bennar, L
dc.contributor.author	Mahmodi, H
dc.contributor.author	Wyllie, K
dc.contributor.author	Idais, D
dc.contributor.author	Al Shamery, W
dc.contributor.author	Paolino, D
dc.contributor.author	Cox, TR
dc.contributor.author	Kabakova, I https://orcid.org/0000-0002-6831-9478
dc.contributor.author	Ralph, P https://orcid.org/0000-0002-3103-7346
dc.contributor.author	Gentile, C https://orcid.org/0000-0002-3689-4275
dc.date.accessioned	2025-01-23T00:33:06Z
dc.date.available	2024-10-02
dc.date.available	2025-01-23T00:33:06Z
dc.date.issued	2024-10-24
dc.identifier.citation	Biofabrication, 2024, 17, (1)
dc.identifier.issn	1758-5082
dc.identifier.issn	1758-5090
dc.identifier.uri	http://hdl.handle.net/10453/184034
dc.description.abstract	Microalgae have emerged as promising photosynthetic microorganisms for biofabricating advanced tissue constructs, with improved oxygenation and reduced reactive oxygen species (ROS) production. However, their use in the engineering of human tissues has been limited due to their intrinsic growth requirements, which are not compatible with human cells. In this study, we first formulated alginate-gelatin (AlgGel) hydrogels with increasing densities ofChlorella vulgaris. Then, we characterised their mechanical properties and pore size. Finally, we evaluated their effects on cardiac spheroid (CS) pathophysiological response under control and ischemia/reperfusion (I/R) conditions. Our results showed that the addition ofChlorelladid not affect AlgGel mechanical properties, while the mean pore size significantly decreased by 35% in the presence of the 107cells ml-1microalgae density. Under normoxic conditions, the addition of 107Chlorellacells ml-1significantly reduced CS viability starting from 14 d in. No changes in pore size nor CS viability were measured for hydrogels containing 105and 106Chlorellacells ml-1. In our I/R model, allChlorella-enriched hydrogels reduced cardiac cell sensitivity to hypoxic conditions with a corresponding reduction in ROS production, as well as protected against I/R-induced reduction in cell viability. Altogether, our results support a promising use ofChlorella-enriched Alg-Gel hydrogels for cardiovascular tissue engineering.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	IOP Publishing Ltd
dc.relation	http://purl.org/au-research/grants/arc/CE230100021
dc.relation	http://purl.org/au-research/grants/arc/CE230100006
dc.relation.ispartof	Biofabrication
dc.relation.isbasedon	10.1088/1758-5090/ad8266
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0903 Biomedical Engineering, 1004 Medical Biotechnology, 1099 Other Technology
dc.subject.classification	3206 Medical biotechnology
dc.subject.classification	4003 Biomedical engineering
dc.subject.mesh	Hydrogels
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Animals
dc.subject.mesh	Alginates
dc.subject.mesh	Chlorella
dc.subject.mesh	Myocytes, Cardiac
dc.subject.mesh	Gelatin
dc.subject.mesh	Cell Survival
dc.subject.mesh	Myocardial Reperfusion Injury
dc.subject.mesh	Myocardium
dc.subject.mesh	Humans
dc.subject.mesh	Rats
dc.subject.mesh	Tissue Engineering
dc.subject.mesh	Hydrogels
dc.subject.mesh	Reactive Oxygen Species
dc.subject.mesh	Spheroids, Cellular
dc.subject.mesh	Animals
dc.subject.mesh	Alginates
dc.subject.mesh	Chlorella
dc.subject.mesh	Myocytes, Cardiac
dc.subject.mesh	Gelatin
dc.subject.mesh	Cell Survival
dc.subject.mesh	Myocardial Reperfusion Injury
dc.subject.mesh	Myocardium
dc.subject.mesh	Humans
dc.subject.mesh	Rats
dc.subject.mesh	Tissue Engineering
dc.title	Chlorella-enriched hydrogels protect against myocardial damage and reactive oxygen species production in anin vitroischemia/reperfusion model using cardiac spheroids.
dc.type	Journal Article
utslib.citation.volume	17
utslib.location.activity	England
utslib.for	0903 Biomedical Engineering
utslib.for	1004 Medical Biotechnology
utslib.for	1099 Other Technology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Biomedical Engineering
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Health Technologies (CHT)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Climate Change Cluster Research Strength (C3)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Institute of Biomedical Materials and Devices (IBMD)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Technology in Water and Wastewater (CTWW)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Advanced Manufacturing (CAM)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Advanced Manufacturing (CAM)/Centre for Advanced Manufacturing (CAM) Associate Members
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Technology in Water and Wastewater (CTWW)/Centre for Technology in Water and Wastewater (CTWW) Associate Members
pubs.organisational-group	University of Technology Sydney/UTS Groups/Institute of Biomedical Materials and Devices (IBMD)/Institute of Biomedical Materials and Devices (IBMD) Associate Members
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-01-23T00:33:04Z
pubs.issue	1
pubs.publication-status	Published online
pubs.volume	17
utslib.citation.issue	1

Abstract:

Microalgae have emerged as promising photosynthetic microorganisms for biofabricating advanced tissue constructs, with improved oxygenation and reduced reactive oxygen species (ROS) production. However, their use in the engineering of human tissues has been limited due to their intrinsic growth requirements, which are not compatible with human cells. In this study, we first formulated alginate-gelatin (AlgGel) hydrogels with increasing densities ofChlorella vulgaris. Then, we characterised their mechanical properties and pore size. Finally, we evaluated their effects on cardiac spheroid (CS) pathophysiological response under control and ischemia/reperfusion (I/R) conditions. Our results showed that the addition ofChlorelladid not affect AlgGel mechanical properties, while the mean pore size significantly decreased by 35% in the presence of the 107cells ml-1microalgae density. Under normoxic conditions, the addition of 107Chlorellacells ml-1significantly reduced CS viability starting from 14 d in. No changes in pore size nor CS viability were measured for hydrogels containing 105and 106Chlorellacells ml-1. In our I/R model, allChlorella-enriched hydrogels reduced cardiac cell sensitivity to hypoxic conditions with a corresponding reduction in ROS production, as well as protected against I/R-induced reduction in cell viability. Altogether, our results support a promising use ofChlorella-enriched Alg-Gel hydrogels for cardiovascular tissue engineering.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/184034