Sucrose non-fermenting 1-related protein kinase 2–14 participating in lipid elevating efficacy and biodiesel upgrade by Coccomyxa subllipsoidea

Luo, Y; Zhao, S; Fan, Z; Li, Y; Peng, Z; Zhang, Y; Feng, S; Mou, J; Wang, Z; Ki Lin, CS; Li, X

Sucrose non-fermenting 1-related protein kinase 2–14 participating in lipid elevating efficacy and biodiesel upgrade by Coccomyxa subllipsoidea

Luo, Y Zhao, S Fan, Z Li, Y Peng, Z Zhang, Y Feng, S

Mou, J Wang, Z

Ki Lin, CS Li, X

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Publisher:: ELSEVIER SCIENCE SA
Publication Type:: Journal Article
Citation:: Chemical Engineering Journal, 2025, 505
Issue Date:: 2025-02-01

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Field	Value	Language
dc.contributor.author	Luo, Y
dc.contributor.author	Zhao, S
dc.contributor.author	Fan, Z
dc.contributor.author	Li, Y
dc.contributor.author	Peng, Z
dc.contributor.author	Zhang, Y
dc.contributor.author	Feng, S https://orcid.org/0000-0002-0051-0751
dc.contributor.author	Mou, J
dc.contributor.author	Wang, Z https://orcid.org/0000-0002-8227-2602
dc.contributor.author	Ki Lin, CS
dc.contributor.author	Li, X https://orcid.org/0000-0003-1768-9556
dc.date.accessioned	2025-04-14T00:34:54Z
dc.date.available	2025-04-14T00:34:54Z
dc.date.issued	2025-02-01
dc.identifier.citation	Chemical Engineering Journal, 2025, 505
dc.identifier.issn	1385-8947
dc.identifier.issn	1873-3212
dc.identifier.uri	http://hdl.handle.net/10453/186887
dc.description.abstract	Microalgae-based biodiesel offered a promising solution to mitigate environmental and energy crises, yet improving lipid productivity and upgrading biodiesel quality in microalgae remained a challenge. Abscisic acid (ABA) supplementation to Coccomyxa subllipsoidea resulted in lipid accumulation, and most sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family genes especially SnRK2-14 containing cis-acting element for ABA responsiveness in region of promoter showed significant up-regulation and strongly positive correlated with lipid productivity. Therefore, this study was the first to explore the lipid elevating efficacy and biodiesel upgrade by SnRK2-14 overexpression in C. subllipsoidea. The lipid yield of SnRK2-14 overexpression C. subllipsoidea strain (SnRK2-14-OCS) boosted to 2.18 g/L, representing a 2.63-fold improvement over wild type (WT). Further, SnRK2-14 overexpression up-regulated lipid-related genes, rewired intermediates/energy derived from protein and carbohydrate degradation, and inhibited lipid oxidation by strengthening antioxidant capacity to collectively achieve lipid overaccumulation in SnRK2-14-OCS. Under fed-batch fermentation mode, the lipid yield further increased 7.92-fold in SnRK2-14-OCS compared to WT and also modulated fatty acid profiles to upgrade biodiesel quality to meet the established quality criteria. These findings revealed the involvement of SnRK2-14 in lipid biosynthesis in microalgae and highlighted the potential of manipulating SnRK2-14 for improved lipid and biodiesel production.
dc.language	English
dc.publisher	ELSEVIER SCIENCE SA
dc.relation.ispartof	Chemical Engineering Journal
dc.relation.isbasedon	10.1016/j.cej.2025.159607
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0904 Chemical Engineering, 0905 Civil Engineering, 0907 Environmental Engineering
dc.subject.classification	Chemical Engineering
dc.subject.classification	4004 Chemical engineering
dc.subject.classification	4011 Environmental engineering
dc.subject.classification	4016 Materials engineering
dc.title	Sucrose non-fermenting 1-related protein kinase 2–14 participating in lipid elevating efficacy and biodiesel upgrade by Coccomyxa subllipsoidea
dc.type	Journal Article
utslib.citation.volume	505
utslib.for	0904 Chemical Engineering
utslib.for	0905 Civil Engineering
utslib.for	0907 Environmental Engineering
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	University of Technology Sydney/UTS Groups
pubs.organisational-group	University of Technology Sydney/UTS Groups/Climate Change & Health Research Collaborative (CCHRC)
pubs.organisational-group	University of Technology Sydney/UTS Groups/Centre for Technology in Water and Wastewater (CTWW)
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-04-14T00:34:51Z
pubs.publication-status	Published
pubs.volume	505

Abstract:

Microalgae-based biodiesel offered a promising solution to mitigate environmental and energy crises, yet improving lipid productivity and upgrading biodiesel quality in microalgae remained a challenge. Abscisic acid (ABA) supplementation to Coccomyxa subllipsoidea resulted in lipid accumulation, and most sucrose non-fermenting 1-related protein kinase 2 (SnRK2) family genes especially SnRK2-14 containing cis-acting element for ABA responsiveness in region of promoter showed significant up-regulation and strongly positive correlated with lipid productivity. Therefore, this study was the first to explore the lipid elevating efficacy and biodiesel upgrade by SnRK2-14 overexpression in C. subllipsoidea. The lipid yield of SnRK2-14 overexpression C. subllipsoidea strain (SnRK2-14-OCS) boosted to 2.18 g/L, representing a 2.63-fold improvement over wild type (WT). Further, SnRK2-14 overexpression up-regulated lipid-related genes, rewired intermediates/energy derived from protein and carbohydrate degradation, and inhibited lipid oxidation by strengthening antioxidant capacity to collectively achieve lipid overaccumulation in SnRK2-14-OCS. Under fed-batch fermentation mode, the lipid yield further increased 7.92-fold in SnRK2-14-OCS compared to WT and also modulated fatty acid profiles to upgrade biodiesel quality to meet the established quality criteria. These findings revealed the involvement of SnRK2-14 in lipid biosynthesis in microalgae and highlighted the potential of manipulating SnRK2-14 for improved lipid and biodiesel production.

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http://hdl.handle.net/10453/186887