The incidence of donor white blood cell survival (transfusion‐associated microchimerism) in Australian pediatric patients

Hirani, R; Ross, B; Ma, Y; Irish, K; Chamberlain, J; Becker, T; Smalley, A; Irving, H; Irving, DO

The incidence of donor white blood cell survival (transfusion‐associated microchimerism) in Australian pediatric patients

Hirani, R Ross, B Ma, Y Irish, K Chamberlain, J Becker, T Smalley, A Irving, H Irving, DO

Permalink

Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Transfusion, 2024, 64, (10), pp. 1830-1840
Issue Date:: 2024-10

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (1.24 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Hirani, R
dc.contributor.author	Ross, B
dc.contributor.author	Ma, Y
dc.contributor.author	Irish, K
dc.contributor.author	Chamberlain, J
dc.contributor.author	Becker, T
dc.contributor.author	Smalley, A
dc.contributor.author	Irving, H
dc.contributor.author	Irving, DO
dc.date.accessioned	2025-06-20T20:48:24Z
dc.date.available	2024-08-25
dc.date.available	2025-06-20T20:48:24Z
dc.date.issued	2024-10
dc.identifier.citation	Transfusion, 2024, 64, (10), pp. 1830-1840
dc.identifier.issn	0041-1132
dc.identifier.issn	1537-2995
dc.identifier.uri	http://hdl.handle.net/10453/187823
dc.description.abstract	INTRODUCTION Donor leucocyte survival following red blood cell (RBC) transfusion, known as transfusion-associated microchimerism (TAM), can occur in some patients. In Australia, despite the introduction of leucocyte filtration (leucodepletion) during RBC manufacture, TAM has been detected in adult trauma patients. However, the incidence of TAM in Australian pediatric patients has not been analyzed. METHODS Patients aged 0-16 years were recruited across two cohorts. Retrospective participants had RBC transfusion between January 1, 2002 and November 15, 2017 and prospective participants received RBC transfusion between December 1, 2016 and November 25, 2020. Twelve bi-allelic insertion/deletion (InDel) polymorphisms were used to detect microchimerism amplification patterns using real-time PCR (RT-PCR) and droplet digital PCR (ddPCR). RESULTS Of the retrospective cohort (n 40), six patients showed amplification of InDel sequences indicating potential microchimerism. For three patients, minor InDel sequences were detected using RT-PCR only, two patients had minor InDel amplification using ddPCR only, and one patient had minor InDel amplification that was confirmed using both techniques. Amplification of minor sequences occurred in three patients who had received a bone marrow transplant in addition to RBC transfusion. In the prospective cohort (n 25), no InDel amplification indicating potential microchimerism was detected using RT-PCR. DISCUSSION Cell-based therapies had been administered in three patients where microchimerism amplification patterns were detected. Three patients have microchimerism that may be attributed to RBC transfusion. In prospective patients, who received leucodepleted and gamma-irradiated RBC units, no potential microchimerism amplification were detected. ddPCR may be a suitable technique for TAM analysis but requires further evaluation.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Wiley
dc.relation.ispartof	Transfusion
dc.relation.isbasedon	10.1111/trf.18010
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1102 Cardiorespiratory Medicine and Haematology, 1103 Clinical Sciences, 1107 Immunology
dc.subject.classification	Cardiovascular System & Hematology
dc.subject.classification	3201 Cardiovascular medicine and haematology
dc.subject.classification	3202 Clinical sciences
dc.subject.classification	3204 Immunology
dc.subject.mesh	Humans
dc.subject.mesh	Child
dc.subject.mesh	Infant
dc.subject.mesh	Child, Preschool
dc.subject.mesh	Australia
dc.subject.mesh	Adolescent
dc.subject.mesh	Chimerism
dc.subject.mesh	Male
dc.subject.mesh	Female
dc.subject.mesh	Retrospective Studies
dc.subject.mesh	Leukocytes
dc.subject.mesh	Erythrocyte Transfusion
dc.subject.mesh	Incidence
dc.subject.mesh	Infant, Newborn
dc.subject.mesh	Prospective Studies
dc.subject.mesh	Blood Donors
dc.subject.mesh	Leukocytes
dc.subject.mesh	Humans
dc.subject.mesh	Erythrocyte Transfusion
dc.subject.mesh	Incidence
dc.subject.mesh	Retrospective Studies
dc.subject.mesh	Prospective Studies
dc.subject.mesh	Chimerism
dc.subject.mesh	Adolescent
dc.subject.mesh	Child
dc.subject.mesh	Child, Preschool
dc.subject.mesh	Infant
dc.subject.mesh	Infant, Newborn
dc.subject.mesh	Blood Donors
dc.subject.mesh	Australia
dc.subject.mesh	Female
dc.subject.mesh	Male
dc.subject.mesh	Humans
dc.subject.mesh	Child
dc.subject.mesh	Infant
dc.subject.mesh	Child, Preschool
dc.subject.mesh	Australia
dc.subject.mesh	Adolescent
dc.subject.mesh	Chimerism
dc.subject.mesh	Male
dc.subject.mesh	Female
dc.subject.mesh	Retrospective Studies
dc.subject.mesh	Leukocytes
dc.subject.mesh	Erythrocyte Transfusion
dc.subject.mesh	Incidence
dc.subject.mesh	Infant, Newborn
dc.subject.mesh	Prospective Studies
dc.subject.mesh	Blood Donors
dc.title	The incidence of donor white blood cell survival (transfusion‐associated microchimerism) in Australian pediatric patients
dc.type	Journal Article
utslib.citation.volume	64
utslib.location.activity	United States
utslib.for	1102 Cardiorespiratory Medicine and Haematology
utslib.for	1103 Clinical Sciences
utslib.for	1107 Immunology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Health
utslib.copyright.status	open_access	*
pubs.consider-herdc	true
dc.rights.license	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated	2025-06-20T20:48:22Z
pubs.issue	10
pubs.publication-status	Published
pubs.volume	64
utslib.citation.issue	10

Abstract:

INTRODUCTION Donor leucocyte survival following red blood cell (RBC) transfusion, known as transfusion-associated microchimerism (TAM), can occur in some patients. In Australia, despite the introduction of leucocyte filtration (leucodepletion) during RBC manufacture, TAM has been detected in adult trauma patients. However, the incidence of TAM in Australian pediatric patients has not been analyzed. METHODS Patients aged 0-16 years were recruited across two cohorts. Retrospective participants had RBC transfusion between January 1, 2002 and November 15, 2017 and prospective participants received RBC transfusion between December 1, 2016 and November 25, 2020. Twelve bi-allelic insertion/deletion (InDel) polymorphisms were used to detect microchimerism amplification patterns using real-time PCR (RT-PCR) and droplet digital PCR (ddPCR). RESULTS Of the retrospective cohort (n 40), six patients showed amplification of InDel sequences indicating potential microchimerism. For three patients, minor InDel sequences were detected using RT-PCR only, two patients had minor InDel amplification using ddPCR only, and one patient had minor InDel amplification that was confirmed using both techniques. Amplification of minor sequences occurred in three patients who had received a bone marrow transplant in addition to RBC transfusion. In the prospective cohort (n 25), no InDel amplification indicating potential microchimerism was detected using RT-PCR. DISCUSSION Cell-based therapies had been administered in three patients where microchimerism amplification patterns were detected. Three patients have microchimerism that may be attributed to RBC transfusion. In prospective patients, who received leucodepleted and gamma-irradiated RBC units, no potential microchimerism amplification were detected. ddPCR may be a suitable technique for TAM analysis but requires further evaluation.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/187823