Taguchi L9 Optimization of Microwave-Assisted Solid-Liquid Duo Heating for Berberine Extraction With HPTLC Analysis.

Satija, S; Vyas, M; Pandey, P; Garg, M

Taguchi L9 Optimization of Microwave-Assisted Solid-Liquid Duo Heating for Berberine Extraction With HPTLC Analysis.

Satija, S

Vyas, M Pandey, P Garg, M

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Biomed Chromatogr, 2025, 39, (3), pp. e70008
Issue Date:: 2025-03

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Field	Value	Language
dc.contributor.author	Satija, S https://orcid.org/0000-0003-4702-6534
dc.contributor.author	Vyas, M
dc.contributor.author	Pandey, P
dc.contributor.author	Garg, M
dc.date.accessioned	2025-07-25T01:47:39Z
dc.date.available	2025-01-14
dc.date.available	2025-07-25T01:47:39Z
dc.date.issued	2025-03
dc.identifier.citation	Biomed Chromatogr, 2025, 39, (3), pp. e70008
dc.identifier.issn	0269-3879
dc.identifier.issn	1099-0801
dc.identifier.uri	http://hdl.handle.net/10453/188727
dc.description.abstract	We present an optimized approach for the extraction of berberine using microwave-assisted solid-liquid duo heating. The conventional procedure relies on the simultaneous heating of the sample matrix and the dissolving solvent under microwave energy. To improve the efficiency of this process, we employed the Taguchi L9 orthogonal design methodology, focusing on key extraction parameters, including microwave power, irradiation time, solute-solvent ratio, and temperature. Berberine content was quantified using high-performance thin-layer chromatography (HPTLC). Our findings revealed that the most influential parameters in the berberine extraction process were microwave power (ranked as the most critical factor), followed by irradiation time (second in importance), and temperature (third in significance). Utilizing the dual heating approach in microwave-assisted extraction (MAE) led to a more precise and efficient determination of extraction parameters, resulting in enhanced accuracy and higher yield. Additionally, this optimized method significantly reduced the overall extraction time, making it an ideal choice for berberine extraction under optimal conditions.
dc.format	Print
dc.language	eng
dc.publisher	Wiley
dc.relation.ispartof	Biomed Chromatogr
dc.relation.isbasedon	10.1002/bmc.70008
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0301 Analytical Chemistry, 1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Analytical Chemistry
dc.subject.classification	3214 Pharmacology and pharmaceutical sciences
dc.subject.classification	3401 Analytical chemistry
dc.subject.mesh	Berberine
dc.subject.mesh	Microwaves
dc.subject.mesh	Chromatography, Thin Layer
dc.subject.mesh	Reproducibility of Results
dc.subject.mesh	Linear Models
dc.subject.mesh	Limit of Detection
dc.subject.mesh	Chromatography, High Pressure Liquid
dc.subject.mesh	Solid Phase Extraction
dc.subject.mesh	Berberine
dc.subject.mesh	Chromatography, High Pressure Liquid
dc.subject.mesh	Chromatography, Thin Layer
dc.subject.mesh	Linear Models
dc.subject.mesh	Reproducibility of Results
dc.subject.mesh	Microwaves
dc.subject.mesh	Solid Phase Extraction
dc.subject.mesh	Limit of Detection
dc.subject.mesh	Berberine
dc.subject.mesh	Microwaves
dc.subject.mesh	Chromatography, Thin Layer
dc.subject.mesh	Reproducibility of Results
dc.subject.mesh	Chromatography, High Pressure Liquid
dc.subject.mesh	Linear Models
dc.subject.mesh	Limit of Detection
dc.title	Taguchi L9 Optimization of Microwave-Assisted Solid-Liquid Duo Heating for Berberine Extraction With HPTLC Analysis.
dc.type	Journal Article
utslib.citation.volume	39
utslib.location.activity	England
utslib.for	0301 Analytical Chemistry
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-07-25T01:47:38Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	39
utslib.citation.issue	3

Abstract:

We present an optimized approach for the extraction of berberine using microwave-assisted solid-liquid duo heating. The conventional procedure relies on the simultaneous heating of the sample matrix and the dissolving solvent under microwave energy. To improve the efficiency of this process, we employed the Taguchi L9 orthogonal design methodology, focusing on key extraction parameters, including microwave power, irradiation time, solute-solvent ratio, and temperature. Berberine content was quantified using high-performance thin-layer chromatography (HPTLC). Our findings revealed that the most influential parameters in the berberine extraction process were microwave power (ranked as the most critical factor), followed by irradiation time (second in importance), and temperature (third in significance). Utilizing the dual heating approach in microwave-assisted extraction (MAE) led to a more precise and efficient determination of extraction parameters, resulting in enhanced accuracy and higher yield. Additionally, this optimized method significantly reduced the overall extraction time, making it an ideal choice for berberine extraction under optimal conditions.

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http://hdl.handle.net/10453/188727