Potential Role of Menstrual Fluid-Derived Small Extracellular Vesicle Proteins in Endometriosis Pathogenesiss.

Gurung, S; Piskopos, J; Steele, J; Schittenhelm, R; Shah, A; Cousins, FL; Tapmeier, TT; Gargett, CE

Potential Role of Menstrual Fluid-Derived Small Extracellular Vesicle Proteins in Endometriosis Pathogenesiss.

Gurung, S Piskopos, J Steele, J

Schittenhelm, R Shah, A Cousins, FL Tapmeier, TT Gargett, CE

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: J Extracell Vesicles, 2025, 14, (3), pp. e70048
Issue Date:: 2025-03

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Field	Value	Language
dc.contributor.author	Gurung, S
dc.contributor.author	Piskopos, J
dc.contributor.author	Steele, J https://orcid.org/0000-0002-3070-9761
dc.contributor.author	Schittenhelm, R
dc.contributor.author	Shah, A
dc.contributor.author	Cousins, FL
dc.contributor.author	Tapmeier, TT
dc.contributor.author	Gargett, CE
dc.date.accessioned	2025-07-25T01:55:17Z
dc.date.available	2025-02-03
dc.date.available	2025-07-25T01:55:17Z
dc.date.issued	2025-03
dc.identifier.citation	J Extracell Vesicles, 2025, 14, (3), pp. e70048
dc.identifier.issn	2001-3078
dc.identifier.issn	2001-3078
dc.identifier.uri	http://hdl.handle.net/10453/188748
dc.description.abstract	Endometriosis, a chronic debilitating disease affects 1 in 7-10 girls and women, who have symptoms of severe chronic pain and subfertility and significantly impacts the overall quality of life. Currently, no effective early diagnostic methods are available for early stages of endometriosis. We used menstrual fluid-derived small extracellular vesicles (MF-sEVs) from women with self-reported endometriosis (laparoscopically diagnosed, n = 8) and self-reported without endometriosis and no painful periods (n = 9). MF-sEVs were separated using differential ultracentrifugation and characterised using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), Western Blot, flow cytometry, mass-proteomics analysis and functional assays. Spherical-shaped sEVs were identified with a median diameter of ∼120 nm, expressing sEV marker proteins. The MF-sEV proteins were classified as endometrial origin. Over 5000 proteins were identified, ∼77% of which were decreased whilst only 22 proteins (largely comprising immunoglobulins) were increased in endometriosis/MF-sEVs compared to control/MF-sEVs. Decreased proteins were involved in nitrogen compound metabolism, immune response, intracellular signal transduction, regulation of programmed cell death, maintenance of cell polarity and actin cytoskeleton organisation. Flow cytometry demonstrated a significant increase in CD86 expression (immune activation marker) in endometriosis/MF-sEVs. Mesothelial cells showed a significant decrease in cellular resistance and junctional protein expression. MF-sEVs are possible contributors to the pathogenesis of endometriosis and may have the potential for early detection of the disease.
dc.format	Print
dc.language	eng
dc.publisher	WILEY
dc.relation.ispartof	J Extracell Vesicles
dc.relation.isbasedon	10.1002/jev2.70048
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology
dc.subject.classification	3101 Biochemistry and cell biology
dc.subject.mesh	Humans
dc.subject.mesh	Female
dc.subject.mesh	Endometriosis
dc.subject.mesh	Extracellular Vesicles
dc.subject.mesh	Adult
dc.subject.mesh	Proteomics
dc.subject.mesh	Menstruation
dc.subject.mesh	Biomarkers
dc.subject.mesh	Humans
dc.subject.mesh	Endometriosis
dc.subject.mesh	Proteomics
dc.subject.mesh	Menstruation
dc.subject.mesh	Adult
dc.subject.mesh	Female
dc.subject.mesh	Biomarkers
dc.subject.mesh	Extracellular Vesicles
dc.subject.mesh	Humans
dc.subject.mesh	Female
dc.subject.mesh	Endometriosis
dc.subject.mesh	Extracellular Vesicles
dc.subject.mesh	Adult
dc.subject.mesh	Proteomics
dc.subject.mesh	Menstruation
dc.subject.mesh	Biomarkers
dc.title	Potential Role of Menstrual Fluid-Derived Small Extracellular Vesicle Proteins in Endometriosis Pathogenesiss.
dc.type	Journal Article
utslib.citation.volume	14
utslib.location.activity	United States
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Provost
pubs.organisational-group	University of Technology Sydney/Provost/Jumbunna
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.date.updated	2025-07-25T01:55:15Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	14
utslib.citation.issue	3

Abstract:

Endometriosis, a chronic debilitating disease affects 1 in 7-10 girls and women, who have symptoms of severe chronic pain and subfertility and significantly impacts the overall quality of life. Currently, no effective early diagnostic methods are available for early stages of endometriosis. We used menstrual fluid-derived small extracellular vesicles (MF-sEVs) from women with self-reported endometriosis (laparoscopically diagnosed, n = 8) and self-reported without endometriosis and no painful periods (n = 9). MF-sEVs were separated using differential ultracentrifugation and characterised using nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), Western Blot, flow cytometry, mass-proteomics analysis and functional assays. Spherical-shaped sEVs were identified with a median diameter of ∼120 nm, expressing sEV marker proteins. The MF-sEV proteins were classified as endometrial origin. Over 5000 proteins were identified, ∼77% of which were decreased whilst only 22 proteins (largely comprising immunoglobulins) were increased in endometriosis/MF-sEVs compared to control/MF-sEVs. Decreased proteins were involved in nitrogen compound metabolism, immune response, intracellular signal transduction, regulation of programmed cell death, maintenance of cell polarity and actin cytoskeleton organisation. Flow cytometry demonstrated a significant increase in CD86 expression (immune activation marker) in endometriosis/MF-sEVs. Mesothelial cells showed a significant decrease in cellular resistance and junctional protein expression. MF-sEVs are possible contributors to the pathogenesis of endometriosis and may have the potential for early detection of the disease.

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http://hdl.handle.net/10453/188748