Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (<i>Leptospermum petersonii)</i> leaves and their cytotoxic effects

Hoang, PL; Castorina, A; Cheng, YY; Van Vuong, Q

Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (<i>Leptospermum petersonii)</i> leaves and their cytotoxic effects

Hoang, PL Castorina, A

Cheng, YY Van Vuong, Q

Permalink

Publisher:: TAYLOR & FRANCIS LTD
Publication Type:: Journal Article
Citation:: COGENT FOOD & AGRICULTURE, 2025, 11, (1)
Issue Date:: 2025-08-06

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published versionAdobe PDF (2.73 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Hoang, PL
dc.contributor.author	Castorina, A https://orcid.org/0000-0001-7037-759X
dc.contributor.author	Cheng, YY
dc.contributor.author	Van Vuong, Q
dc.date.accessioned	2025-09-03T07:51:58Z
dc.date.available	2025-09-03T07:51:58Z
dc.date.issued	2025-08-06
dc.identifier.citation	COGENT FOOD & AGRICULTURE, 2025, 11, (1)
dc.identifier.issn	2331-1932
dc.identifier.issn	2331-1932
dc.identifier.uri	http://hdl.handle.net/10453/189605
dc.description.abstract	The lemon-scented tea tree (Leptospermum petersonii) is rich in bioactive compounds with therapeutic potential; however, optimal extraction conditions for these compounds remain undefined. This study systematically optimised reflux extraction for 16 key bioactives from L. petersonii leaves. Solvent type and extraction conditions significantly influenced extraction yields, with 60% ethanol identified as the most effective solvent. Response surface methodology robustly modelled the effects of temperature, reflux time, and sample-to-solvent ratio (lack of fit = 0.1773; p = 0.0069; R² = 0.96). The predicted optimal conditions (95 °C, 160 min, 6:100 g/mL) yielded 39 mg/g of total bioactives. For improved cost-efficiency, extraction at 95 °C for 180 min with a 10:100 g/mL ratio was recommended, reducing solvent use by 40% with only an 8% yield loss, producing 35.8 mg/g bioactives. This extract exhibited strong cytotoxicity against prostate cancer (LNCaP), melanoma (A375, COLO 679), and mesothelioma (AC29) cell lines, with IC₅。 values < 2.50 μg/mL, while showing reduced toxicity toward non-cancerous BV2 cells. These findings establish efficient, scalable extraction conditions and highlight the anticancer potential of L. petersonii, warranting further isolation and mechanistic studies of its active constituents.
dc.language	English
dc.publisher	TAYLOR & FRANCIS LTD
dc.relation.ispartof	COGENT FOOD & AGRICULTURE
dc.relation.isbasedon	10.1080/23311932.2025.2544962
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	3002 Agriculture, land and farm management
dc.subject.classification	3003 Animal production
dc.subject.classification	3006 Food sciences
dc.title	Optimum heat reflux extraction of key bioactive compounds from lemon scented tea tree (<i>Leptospermum petersonii)</i> leaves and their cytotoxic effects
dc.type	Journal Article
utslib.citation.volume	11
pubs.organisational-group	University of Technology Sydney
pubs.organisational-group	University of Technology Sydney/Faculty of Science
pubs.organisational-group	University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.rights.license	This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0). To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/
dc.date.updated	2025-09-03T07:51:56Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	11
utslib.citation.issue	1

Abstract:

The lemon-scented tea tree (Leptospermum petersonii) is rich in bioactive compounds with therapeutic potential; however, optimal extraction conditions for these compounds remain undefined. This study systematically optimised reflux extraction for 16 key bioactives from L. petersonii leaves. Solvent type and extraction conditions significantly influenced extraction yields, with 60% ethanol identified as the most effective solvent. Response surface methodology robustly modelled the effects of temperature, reflux time, and sample-to-solvent ratio (lack of fit = 0.1773; p = 0.0069; R² = 0.96). The predicted optimal conditions (95 °C, 160 min, 6:100 g/mL) yielded 39 mg/g of total bioactives. For improved cost-efficiency, extraction at 95 °C for 180 min with a 10:100 g/mL ratio was recommended, reducing solvent use by 40% with only an 8% yield loss, producing 35.8 mg/g bioactives. This extract exhibited strong cytotoxicity against prostate cancer (LNCaP), melanoma (A375, COLO 679), and mesothelioma (AC29) cell lines, with IC₅。 values < 2.50 μg/mL, while showing reduced toxicity toward non-cancerous BV2 cells. These findings establish efficient, scalable extraction conditions and highlight the anticancer potential of L. petersonii, warranting further isolation and mechanistic studies of its active constituents.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/189605