Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria

Burke, C; Liu, M; Britton, W; Triccas, JA; Thomas, T; Smith, AL; Allen, S; Salomon, R; Harry, E

Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria

Burke, C

Liu, M

Britton, W Triccas, JA Thomas, T Smith, AL Allen, S Salomon, R

Harry, E

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Publication Type:: Journal Article
Citation:: PLoS ONE, 2013, 8 (4)
Issue Date:: 2013-04-02

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Field	Value	Language
dc.contributor.author	Burke, C https://orcid.org/0000-0002-8917-0019	en_US
dc.contributor.author	Liu, M https://orcid.org/0000-0001-8312-0734	en_US
dc.contributor.author	Britton, W	en_US
dc.contributor.author	Triccas, JA	en_US
dc.contributor.author	Thomas, T	en_US
dc.contributor.author	Smith, AL	en_US
dc.contributor.author	Allen, S	en_US
dc.contributor.author	Salomon, R https://orcid.org/0000-0003-4504-2901	en_US
dc.contributor.author	Harry, E https://orcid.org/0000-0003-0858-9473	en_US
dc.date.available	2013-03-04	en_US
dc.date.issued	2013-04-02	en_US
dc.identifier.citation	PLoS ONE, 2013, 8 (4)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/27447
dc.description.abstract	Cell division is an essential cellular process that requires an array of known and unknown proteins for its spatial and temporal regulation. Here we develop a novel, high-throughput screening method for the identification of bacterial cell division genes and regulators. The method combines the over-expression of a shotgun genomic expression library to perturb the cell division process with high-throughput flow cytometry sorting to screen many thousands of clones. Using this approach, we recovered clones with a filamentous morphology for the model bacterium, Escherichia coli. Genetic analysis revealed that our screen identified both known cell division genes, and genes that have not previously been identified to be involved in cell division. This novel screening strategy is applicable to a wide range of organisms, including pathogenic bacteria, where cell division genes and regulators are attractive drug targets for antibiotic development. © 2013 Burke et al.	en_US
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0060964	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Flow Cytometry	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Bacterial Physiological Phenomena	en_US
dc.title	Harnessing Single Cell Sorting to Identify Cell Division Genes and Regulators in Bacteria	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	8	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0604 Genetics	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	8	en_US

Abstract:

Cell division is an essential cellular process that requires an array of known and unknown proteins for its spatial and temporal regulation. Here we develop a novel, high-throughput screening method for the identification of bacterial cell division genes and regulators. The method combines the over-expression of a shotgun genomic expression library to perturb the cell division process with high-throughput flow cytometry sorting to screen many thousands of clones. Using this approach, we recovered clones with a filamentous morphology for the model bacterium, Escherichia coli. Genetic analysis revealed that our screen identified both known cell division genes, and genes that have not previously been identified to be involved in cell division. This novel screening strategy is applicable to a wide range of organisms, including pathogenic bacteria, where cell division genes and regulators are attractive drug targets for antibiotic development. © 2013 Burke et al.

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http://hdl.handle.net/10453/27447