Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T

Wei, J; Goldberg, MB; Burland, V; Venkatesan, MM; Deng, W; Fournier, G; Mayhew, GF; Plunkett, G; Rose, DJ; Darling, A; Mau, B; Perna, NT; Payne, SM; Runyen-Janecky, LJ; Zhou, S; Schwartz, DC; Blattner, FR

Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T

Wei, J Goldberg, MB Burland, V Venkatesan, MM Deng, W Fournier, G Mayhew, GF Plunkett, G Rose, DJ Darling, A

Mau, B Perna, NT Payne, SM Runyen-Janecky, LJ Zhou, S Schwartz, DC Blattner, FR

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Publication Type:: Journal Article
Citation:: Infection and Immunity, 2003, 71 (5), pp. 2775 - 2786
Issue Date:: 2003-05-01

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Field	Value	Language
dc.contributor.author	Wei, J	en_US
dc.contributor.author	Goldberg, MB	en_US
dc.contributor.author	Burland, V	en_US
dc.contributor.author	Venkatesan, MM	en_US
dc.contributor.author	Deng, W	en_US
dc.contributor.author	Fournier, G	en_US
dc.contributor.author	Mayhew, GF	en_US
dc.contributor.author	Plunkett, G	en_US
dc.contributor.author	Rose, DJ	en_US
dc.contributor.author	Darling, A https://orcid.org/0000-0003-2397-7925	en_US
dc.contributor.author	Mau, B	en_US
dc.contributor.author	Perna, NT	en_US
dc.contributor.author	Payne, SM	en_US
dc.contributor.author	Runyen-Janecky, LJ	en_US
dc.contributor.author	Zhou, S	en_US
dc.contributor.author	Schwartz, DC	en_US
dc.contributor.author	Blattner, FR	en_US
dc.date.issued	2003-05-01	en_US
dc.identifier.citation	Infection and Immunity, 2003, 71 (5), pp. 2775 - 2786	en_US
dc.identifier.issn	0019-9567	en_US
dc.identifier.uri	http://hdl.handle.net/10453/27464
dc.description.abstract	We determined the complete genome sequence of Shigella flexneri serotype 2a strain 2457T (4,599,354 bp). Shigella species cause >1 million deaths per year from dysentery and diarrhea and have a lifestyle that is markedly different from those of closely related bacteria, including Escherichia coli. The genome exhibits the backbone and island mosaic structure of E. coli pathogens, albeit with much less horizontally transferred DNA and lacking 357 genes present in E. coli. The strain is distinctive in its large complement of insertion sequences, with several genomic rearrangements mediated by insertion sequences, 12 cryptic prophages, 372 pseudogenes, and 195 S. flexneri-specific genes. The 2457T genome was also compared with that of a recently sequenced S. flexneri 2a strain, 301. Our data are consistent with Shigella being phylogenetically indistinguishable from E. coli. The S. flexneri-specific regions contain many genes that could encode proteins with roles in virulence. Analysis of these will reveal the genetic basis for aspects of this pathogenic organism's distinctive lifestyle that have yet to be explained.	en_US
dc.relation.ispartof	Infection and Immunity	en_US
dc.relation.isbasedon	10.1128/IAI.71.5.2775-2786.2003	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Shigella flexneri	en_US
dc.subject.mesh	DNA Transposable Elements	en_US
dc.subject.mesh	Genomics	en_US
dc.subject.mesh	Phylogeny	en_US
dc.subject.mesh	Base Sequence	en_US
dc.subject.mesh	Genes, Bacterial	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Plasmids	en_US
dc.subject.mesh	Molecular Sequence Data	en_US
dc.title	Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	71	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	06 Biological Sciences	en_US
utslib.for	07 Agricultural and Veterinary Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	71	en_US

Abstract:

We determined the complete genome sequence of Shigella flexneri serotype 2a strain 2457T (4,599,354 bp). Shigella species cause >1 million deaths per year from dysentery and diarrhea and have a lifestyle that is markedly different from those of closely related bacteria, including Escherichia coli. The genome exhibits the backbone and island mosaic structure of E. coli pathogens, albeit with much less horizontally transferred DNA and lacking 357 genes present in E. coli. The strain is distinctive in its large complement of insertion sequences, with several genomic rearrangements mediated by insertion sequences, 12 cryptic prophages, 372 pseudogenes, and 195 S. flexneri-specific genes. The 2457T genome was also compared with that of a recently sequenced S. flexneri 2a strain, 301. Our data are consistent with Shigella being phylogenetically indistinguishable from E. coli. The S. flexneri-specific regions contain many genes that could encode proteins with roles in virulence. Analysis of these will reveal the genetic basis for aspects of this pathogenic organism's distinctive lifestyle that have yet to be explained.

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http://hdl.handle.net/10453/27464