Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux

Petty, NK; Feltwell, T; Pickard, D; Clare, S; Toribio, AL; Fookes, M; Roberts, K; Monson, R; Nair, S; Kingsley, RA; Bulgin, R; Wiles, S; Goulding, D; Keane, T; Corton, C; Lennard, N; Harris, D; Willey, D; Rance, R; Yu, L; Choudhary, JS; Churcher, C; Quail, MA; Parkhill, J; Frankel, G; Dougan, G; Salmond, GPC; Thomson, NR

Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux

Feltwell, T Pickard, D Clare, S Toribio, AL Fookes, M Roberts, K Monson, R Nair, S Kingsley, RA Bulgin, R Wiles, S Goulding, D Keane, T Corton, C Lennard, N Harris, D Willey, D Rance, R Yu, L Choudhary, JS Churcher, C Quail, MA Parkhill, J Frankel, G Dougan, G Salmond, GPC Thomson, NR

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Publication Type:: Journal Article
Citation:: PLoS Pathogens, 2011, 7 (4)
Issue Date:: 2011-04-01

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Field	Value	Language
dc.contributor.author	Petty, NK https://orcid.org/0000-0001-6528-9886	en_US
dc.contributor.author	Feltwell, T	en_US
dc.contributor.author	Pickard, D	en_US
dc.contributor.author	Clare, S	en_US
dc.contributor.author	Toribio, AL	en_US
dc.contributor.author	Fookes, M	en_US
dc.contributor.author	Roberts, K	en_US
dc.contributor.author	Monson, R	en_US
dc.contributor.author	Nair, S	en_US
dc.contributor.author	Kingsley, RA	en_US
dc.contributor.author	Bulgin, R	en_US
dc.contributor.author	Wiles, S	en_US
dc.contributor.author	Goulding, D	en_US
dc.contributor.author	Keane, T	en_US
dc.contributor.author	Corton, C	en_US
dc.contributor.author	Lennard, N	en_US
dc.contributor.author	Harris, D	en_US
dc.contributor.author	Willey, D	en_US
dc.contributor.author	Rance, R	en_US
dc.contributor.author	Yu, L	en_US
dc.contributor.author	Choudhary, JS	en_US
dc.contributor.author	Churcher, C	en_US
dc.contributor.author	Quail, MA	en_US
dc.contributor.author	Parkhill, J	en_US
dc.contributor.author	Frankel, G	en_US
dc.contributor.author	Dougan, G	en_US
dc.contributor.author	Salmond, GPC	en_US
dc.contributor.author	Thomson, NR	en_US
dc.date.available	2011-02-18	en_US
dc.date.issued	2011-04-01	en_US
dc.identifier.citation	PLoS Pathogens, 2011, 7 (4)	en_US
dc.identifier.issn	1553-7366	en_US
dc.identifier.uri	http://hdl.handle.net/10453/27800
dc.description.abstract	Citrobacter rodentium is a natural mouse pathogen that causes attaching and effacing (A/E) lesions. It shares a common virulence strategy with the clinically significant human A/E pathogens enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) and is widely used to model this route of pathogenesis. We previously reported the complete genome sequence of C. rodentium ICC168, where we found that the genome displayed many characteristics of a newly evolved pathogen. In this study, through PFGE, sequencing of isolates showing variation, whole genome transcriptome analysis and examination of the mobile genetic elements, we found that, consistent with our previous hypothesis, the genome of C. rodentium is unstable as a result of repeat-mediated, large-scale genome recombination and because of active transposition of mobile genetic elements such as the prophages. We sequenced an additional C. rodentium strain, EX-33, to reveal that the reference strain ICC168 is representative of the species and that most of the inactivating mutations were common to both isolates and likely to have occurred early on in the evolution of this pathogen. We draw parallels with the evolution of other bacterial pathogens and conclude that C. rodentium is a recently evolved pathogen that may have emerged alongside the development of inbred mice as a model for human disease. © 2011 Petty et al.	en_US
dc.relation.ispartof	PLoS Pathogens	en_US
dc.relation.isbasedon	10.1371/journal.ppat.1002018	en_US
dc.subject.classification	Virology	en_US
dc.subject.mesh	Animals	en_US
dc.subject.mesh	Mice, Inbred C57BL	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Mice	en_US
dc.subject.mesh	Citrobacter rodentium	en_US
dc.subject.mesh	Prophages	en_US
dc.subject.mesh	Enterobacteriaceae Infections	en_US
dc.subject.mesh	Disease Models, Animal	en_US
dc.subject.mesh	Genomic Instability	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Electrophoresis, Gel, Pulsed-Field	en_US
dc.subject.mesh	Gene Expression Profiling	en_US
dc.subject.mesh	Sequence Analysis, DNA	en_US
dc.subject.mesh	Virulence	en_US
dc.subject.mesh	Gene Rearrangement	en_US
dc.subject.mesh	Interspersed Repetitive Sequences	en_US
dc.subject.mesh	Genome, Bacterial	en_US
dc.subject.mesh	Plasmids	en_US
dc.subject.mesh	Female	en_US
dc.title	Citrobacter rodentium is an unstable pathogen showing evidence of significant genomic flux	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.citation.volume	7	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	1107 Immunology	en_US
utslib.for	1108 Medical Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	closed_access
pubs.issue	4	en_US
pubs.publication-status	Published	en_US
pubs.volume	7	en_US

Abstract:

Citrobacter rodentium is a natural mouse pathogen that causes attaching and effacing (A/E) lesions. It shares a common virulence strategy with the clinically significant human A/E pathogens enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) and is widely used to model this route of pathogenesis. We previously reported the complete genome sequence of C. rodentium ICC168, where we found that the genome displayed many characteristics of a newly evolved pathogen. In this study, through PFGE, sequencing of isolates showing variation, whole genome transcriptome analysis and examination of the mobile genetic elements, we found that, consistent with our previous hypothesis, the genome of C. rodentium is unstable as a result of repeat-mediated, large-scale genome recombination and because of active transposition of mobile genetic elements such as the prophages. We sequenced an additional C. rodentium strain, EX-33, to reveal that the reference strain ICC168 is representative of the species and that most of the inactivating mutations were common to both isolates and likely to have occurred early on in the evolution of this pathogen. We draw parallels with the evolution of other bacterial pathogens and conclude that C. rodentium is a recently evolved pathogen that may have emerged alongside the development of inbred mice as a model for human disease. © 2011 Petty et al.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/27800