Optimization of antibiotic analysis in water by solid-phase extraction and high performance liquid chromatography-mass spectrometry/mass spectrometry

Zhou, JL; Maskaoui, K; Lufadeju, A

Optimization of antibiotic analysis in water by solid-phase extraction and high performance liquid chromatography-mass spectrometry/mass spectrometry

Zhou, JL

Maskaoui, K Lufadeju, A

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Publication Type:: Journal Article
Citation:: Analytica Chimica Acta, 2012, 731 pp. 32 - 39
Issue Date:: 2012-06-20

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Field	Value	Language
dc.contributor.author	Zhou, JL https://orcid.org/0000-0003-1393-1608	en_US
dc.contributor.author	Maskaoui, K	en_US
dc.contributor.author	Lufadeju, A	en_US
dc.date.available	2012-04-06	en_US
dc.date.issued	2012-06-20	en_US
dc.identifier.citation	Analytica Chimica Acta, 2012, 731 pp. 32 - 39	en_US
dc.identifier.issn	0003-2670	en_US
dc.identifier.uri	http://hdl.handle.net/10453/28152
dc.identifier.uri	http://hdl.handle.net/10453/33696
dc.description.abstract	This paper describes the development of an optimized method based on solid-phase extraction (SPE) followed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS) for the simultaneous analysis of ten antibiotic compounds including tetracyclines, sulfonamides, macrolides and quinolones. LC-MS/MS sensitivity has been optimized by alterations to both LC and MS operations. Of the two high resolution columns tested, Waters Symmetry C18 endcapped and Agilent Zorbax Bonus-RP, the latter was found to show better performance in producing sharp peaks and clear separation for most of the target compounds. Optimization of the MS fragmentation collision and cone energy enhanced the peak areas of the target analytes. The recovery of the target compounds from water samples was most efficient on Waters Oasis HLB SPE cartridge, while methanol was shown to be the most suitable solvent for desorbing the compounds from SPE. In addition, acidification of samples prior to SPE was shown to enhance the recovery of the compounds. To ensure a satisfactory recovery, the flow rate through SPE should be maintained at ≤10mLmin-1. The method was successfully applied to the analysis of antibiotics from environmental water samples, with concentrations being <LOD in tap water, between <LOD to 28ngL-1 in river water and between <LOD to 230ngL-1 in sewage effluent. © 2012 Elsevier B.V.	en_US
dc.relation.ispartof	Analytica Chimica Acta	en_US
dc.relation.isbasedon	10.1016/j.aca.2012.04.021	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Water	en_US
dc.subject.mesh	Anti-Bacterial Agents	en_US
dc.subject.mesh	Solvents	en_US
dc.subject.mesh	Water Pollutants, Chemical	en_US
dc.subject.mesh	Chromatography, High Pressure Liquid	en_US
dc.subject.mesh	Spectrometry, Mass, Electrospray Ionization	en_US
dc.subject.mesh	Hydrogen-Ion Concentration	en_US
dc.subject.mesh	Time Factors	en_US
dc.subject.mesh	Tandem Mass Spectrometry	en_US
dc.subject.mesh	Solid Phase Extraction	en_US
dc.subject.mesh	Salinity	en_US
dc.title	Optimization of antibiotic analysis in water by solid-phase extraction and high performance liquid chromatography-mass spectrometry/mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	731	en_US
utslib.for	0907 Environmental Engineering	en_US
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	731	en_US

Abstract:

This paper describes the development of an optimized method based on solid-phase extraction (SPE) followed by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-MS/MS) for the simultaneous analysis of ten antibiotic compounds including tetracyclines, sulfonamides, macrolides and quinolones. LC-MS/MS sensitivity has been optimized by alterations to both LC and MS operations. Of the two high resolution columns tested, Waters Symmetry C18 endcapped and Agilent Zorbax Bonus-RP, the latter was found to show better performance in producing sharp peaks and clear separation for most of the target compounds. Optimization of the MS fragmentation collision and cone energy enhanced the peak areas of the target analytes. The recovery of the target compounds from water samples was most efficient on Waters Oasis HLB SPE cartridge, while methanol was shown to be the most suitable solvent for desorbing the compounds from SPE. In addition, acidification of samples prior to SPE was shown to enhance the recovery of the compounds. To ensure a satisfactory recovery, the flow rate through SPE should be maintained at ≤10mLmin-1. The method was successfully applied to the analysis of antibiotics from environmental water samples, with concentrations being

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http://hdl.handle.net/10453/28152