Direct estimation of functional PSII reaction center concentration and PSII electron flux on a volume basis: A new approach to the analysis of Fast Repetition Rate fluorometry (FRRf) data

Oxborough, K; Moore, CM; Suggett, DJ; Lawson, T; Chan, HG; Geider, RJ

Direct estimation of functional PSII reaction center concentration and PSII electron flux on a volume basis: A new approach to the analysis of Fast Repetition Rate fluorometry (FRRf) data

Oxborough, K Moore, CM Suggett, DJ

Lawson, T Chan, HG Geider, RJ

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Publication Type:: Journal Article
Citation:: Limnology and Oceanography: Methods, 2012, 10 (MARCH), pp. 142 - 154
Issue Date:: 2012-06-08

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Field	Value	Language
dc.contributor.author	Oxborough, K	en_US
dc.contributor.author	Moore, CM	en_US
dc.contributor.author	Suggett, DJ https://orcid.org/0000-0001-5326-2520	en_US
dc.contributor.author	Lawson, T	en_US
dc.contributor.author	Chan, HG	en_US
dc.contributor.author	Geider, RJ	en_US
dc.date.issued	2012-06-08	en_US
dc.identifier.citation	Limnology and Oceanography: Methods, 2012, 10 (MARCH), pp. 142 - 154	en_US
dc.identifier.uri	http://hdl.handle.net/10453/29124
dc.description.abstract	Phytoplankton primary productivity is most commonly measured by 14C assimilation although less direct methods, such as O2 exchange, have also been employed. These methods are invasive, requiring bottle incubation for up to 24 h. As an alternative, Fast Repetition Rate fluorometry (FRRf) has been used, on wide temporal and spatial scales within aquatic systems, to estimate photosystem II (PSII) electron flux per unit volume (JVPSII), which generally correlates well with photosynthetic O2 evolution. A major limitation of using FRRf arises from the need to employ an independent method to determine the concentration of functional photosystem II reaction centers ([RCII]); a requirement that has prevented FRR fluorometers being used, as stand-alone instruments, for the estimation of electron transport. Within this study, we have taken a new approach to the analysis of FRRf data, based on a simple hypothesis; that under a given set of environmental conditions, the ratio of rate constants for RCII fluorescence emission and photochemistry falls within a narrow range, for all groups of phytoplankton. We present a simple equation, derived from the established FRRf algorithm, for determining [RCII] from dark FRRf data alone. We also describe an entirely new algorithm for estimating JVPSII, which does not require determination of [RCII] and is valid for a heterogeneous model of connectivity among RCIIs. Empirical supporting evidence is presented. These data are derived from FRR measurements across a diverse range of microalgae, in parallel with independent measurements of [RCII]. Possible sources of error, particularly under nutrient stress conditions, are discussed. © 2012, by the American Society of Limnology and Oceanography, Inc.	en_US
dc.relation.ispartof	Limnology and Oceanography: Methods	en_US
dc.relation.isbasedon	10.4319/lom.2012.10.142	en_US
dc.subject.classification	Marine Biology & Hydrobiology	en_US
dc.title	Direct estimation of functional PSII reaction center concentration and PSII electron flux on a volume basis: A new approach to the analysis of Fast Repetition Rate fluorometry (FRRf) data	en_US
dc.type	Journal Article
utslib.citation.volume	MARCH	en_US
utslib.citation.volume	10	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	04 Earth Sciences	en_US
utslib.for	06 Biological Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	MARCH	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

Phytoplankton primary productivity is most commonly measured by 14C assimilation although less direct methods, such as O2 exchange, have also been employed. These methods are invasive, requiring bottle incubation for up to 24 h. As an alternative, Fast Repetition Rate fluorometry (FRRf) has been used, on wide temporal and spatial scales within aquatic systems, to estimate photosystem II (PSII) electron flux per unit volume (JVPSII), which generally correlates well with photosynthetic O2 evolution. A major limitation of using FRRf arises from the need to employ an independent method to determine the concentration of functional photosystem II reaction centers ([RCII]); a requirement that has prevented FRR fluorometers being used, as stand-alone instruments, for the estimation of electron transport. Within this study, we have taken a new approach to the analysis of FRRf data, based on a simple hypothesis; that under a given set of environmental conditions, the ratio of rate constants for RCII fluorescence emission and photochemistry falls within a narrow range, for all groups of phytoplankton. We present a simple equation, derived from the established FRRf algorithm, for determining [RCII] from dark FRRf data alone. We also describe an entirely new algorithm for estimating JVPSII, which does not require determination of [RCII] and is valid for a heterogeneous model of connectivity among RCIIs. Empirical supporting evidence is presented. These data are derived from FRR measurements across a diverse range of microalgae, in parallel with independent measurements of [RCII]. Possible sources of error, particularly under nutrient stress conditions, are discussed. © 2012, by the American Society of Limnology and Oceanography, Inc.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/29124