High-speed fluorescence imaging and intensity profiling of femtosecond-induced calcium transients

Day, D; Cranfield, CG; Gu, M

High-speed fluorescence imaging and intensity profiling of femtosecond-induced calcium transients

Day, D Cranfield, CG

Gu, M

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Publication Type:: Journal Article
Citation:: International Journal of Biomedical Imaging, 2006, 2006
Issue Date:: 2006-04-11

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Field	Value	Language
dc.contributor.author	Day, D	en_US
dc.contributor.author	Cranfield, CG https://orcid.org/0000-0003-3608-5440	en_US
dc.contributor.author	Gu, M	en_US
dc.date.available	2006-01-05	en_US
dc.date.issued	2006-04-11	en_US
dc.identifier.citation	International Journal of Biomedical Imaging, 2006, 2006	en_US
dc.identifier.issn	1687-4188	en_US
dc.identifier.uri	http://hdl.handle.net/10453/30118
dc.description.abstract	We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10-20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propagating focused femtosecond beam with respect to the imaging system. As a result of the stimulation, calcium waves can be generated in COS cells, and laser-induced calcium oscillations are initiated in the GH3 cells. Single-photon fluorescence images and intensity profiles of the targeted specimens are sampled in real-time using a modified PerkinElmer UltraView LCI microscope. Copyright © 2006 Daniel Day et al.	en_US
dc.relation.ispartof	International Journal of Biomedical Imaging	en_US
dc.relation.isbasedon	10.1155/IJBI/2006/93438	en_US
dc.subject.classification	Nuclear Medicine & Medical Imaging	en_US
dc.title	High-speed fluorescence imaging and intensity profiling of femtosecond-induced calcium transients	en_US
dc.type	Journal Article
utslib.citation.volume	2006	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	2006	en_US

Abstract:

We have demonstrated a combined imaging system, where the physiology of biological specimens can be imaged and profiled at 10-20 frames per second whilst undergoing femtosecond laser irradiation. Individual GH3 cells labeled with the calcium fluorophore Fluo-3 were stimulated using a counter-propagating focused femtosecond beam with respect to the imaging system. As a result of the stimulation, calcium waves can be generated in COS cells, and laser-induced calcium oscillations are initiated in the GH3 cells. Single-photon fluorescence images and intensity profiles of the targeted specimens are sampled in real-time using a modified PerkinElmer UltraView LCI microscope. Copyright © 2006 Daniel Day et al.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/30118