Fish gut content analysis by thermochemolysis with tetramethylammonium hydroxide (TMAH) and gas chromatography-mass spectrometry (GC-MS)

McIntyre, C; Wressnig, A; McRae, C

Fish gut content analysis by thermochemolysis with tetramethylammonium hydroxide (TMAH) and gas chromatography-mass spectrometry (GC-MS)

McIntyre, C Wressnig, A McRae, C

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Publisher:: Elsevier Science Bv
Publication Type:: Journal Article
Citation:: McIntyre, C., Wressing, A., and McRae, C. 2007 'Fish gut content analysis by thermochemolysis with tetramethylammonium hydroxide (TMAH) and gas chromatography-mass spectrometry (GC-MS)', Journal Of Analytical And Applied Pyrolysis, vol. 80, no. 1, pp. 6-15.
Issue Date:: 2007

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Field	Value	Language
dc.contributor.author	McIntyre, C	en_US
dc.contributor.author	Wressnig, A	en_US
dc.contributor.author	McRae, C	en_US
dc.date.accessioned	2009-11-12T04:41:10Z
dc.date.available	2009-11-12T04:41:10Z
dc.date.issued	2007	en_US
dc.identifier	2006011463	en_US
dc.identifier.citation	McIntyre, C., Wressing, A., and McRae, C. 2007 'Fish gut content analysis by thermochemolysis with tetramethylammonium hydroxide (TMAH) and gas chromatography-mass spectrometry (GC-MS)', Journal Of Analytical And Applied Pyrolysis, vol. 80, no. 1, pp. 6-15.	en_US
dc.identifier.issn	0165-2370	en_US
dc.identifier.other	C1	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3215
dc.description.abstract	Gut content analyses of fish are typically conducted using methods based on visual identification. These can lead to inconsistent results because of their subjective nature, especially when sample sizes are limited or food items are not resolvable. New approaches are required to increase the accuracy of gut content analysis. We investigate whether thermochemolysis with TMAH and GC-MS detection can qualitatively and quantitatively analyse the gut contents of two Monacanthid fishes fed seagrass, epiflora and epifauna under controlled conditions. The three food items could be readily differentiated when analysed individually, seagrass could be distinguished from epiphytes (epifauna + epiflora) in the gut, and quantitative data coule be obtained using marker compounds unique to a food item. Thermochemolysis with TMAH and GC-MS represents a new technique for the gut content analysis of fish which can complement traditional techqniques and be samples that are very small (0.5 -2mg) and contain difficult to separate items.	en_US
dc.publisher	Elsevier Science Bv	en_US
dc.relation.ispartof	Verified OK	en_US
dc.relation.ispartof	Journal Of Analytical And Applied Pyrolysis	en_US
dc.relation.isbasedon	http://dx.doi.org/10.1016/j.jaap.2006.12.013	en_US
dc.subject	gut content analysis, TMAH, thermochemolysis, GC-MS, fish	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.title	Fish gut content analysis by thermochemolysis with tetramethylammonium hydroxide (TMAH) and gas chromatography-mass spectrometry (GC-MS)	en_US
dc.type	Journal article
utslib.citation.volume	80	en_US
utslib.citation.number	1	en_US
utslib.location	Amsterdam, Netherlands	en_US
utslib.citation.startpage	6	en_US
utslib.citation.endpage	15	en_US
utslib.identifier.org	SCI.Faculty of Science	en_US
utslib.for	030100	en_US
utslib.percentage	100	en_US
utslib.copyright.status	closed_access
pubs.declined	1970-01-01T00:00:00.0+1000

Abstract:

Gut content analyses of fish are typically conducted using methods based on visual identification. These can lead to inconsistent results because of their subjective nature, especially when sample sizes are limited or food items are not resolvable. New approaches are required to increase the accuracy of gut content analysis. We investigate whether thermochemolysis with TMAH and GC-MS detection can qualitatively and quantitatively analyse the gut contents of two Monacanthid fishes fed seagrass, epiflora and epifauna under controlled conditions. The three food items could be readily differentiated when analysed individually, seagrass could be distinguished from epiphytes (epifauna + epiflora) in the gut, and quantitative data coule be obtained using marker compounds unique to a food item. Thermochemolysis with TMAH and GC-MS represents a new technique for the gut content analysis of fish which can complement traditional techqniques and be samples that are very small (0.5 -2mg) and contain difficult to separate items.

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http://hdl.handle.net/10453/3215