Insight into small RNA abundance and expression in high- and low-temperature stress response using deep sequencing in Arabidopsis

Baev, V; Milev, I; Naydenov, M; Vachev, T; Apostolova, E; Mehterov, N; Gozmanva, M; Minkov, G; Sablok, G; Yahubyan, G

Insight into small RNA abundance and expression in high- and low-temperature stress response using deep sequencing in Arabidopsis

Baev, V Milev, I Naydenov, M Vachev, T Apostolova, E Mehterov, N Gozmanva, M Minkov, G Sablok, G

Yahubyan, G

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Publication Type:: Journal Article
Citation:: Plant Physiology and Biochemistry, 2014, 84 pp. 105 - 114
Issue Date:: 2014-11-01

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Field	Value	Language
dc.contributor.author	Baev, V	en_US
dc.contributor.author	Milev, I	en_US
dc.contributor.author	Naydenov, M	en_US
dc.contributor.author	Vachev, T	en_US
dc.contributor.author	Apostolova, E	en_US
dc.contributor.author	Mehterov, N	en_US
dc.contributor.author	Gozmanva, M	en_US
dc.contributor.author	Minkov, G	en_US
dc.contributor.author	Sablok, G https://orcid.org/0000-0002-4157-9405	en_US
dc.contributor.author	Yahubyan, G	en_US
dc.date.available	2014-09-15	en_US
dc.date.issued	2014-11-01	en_US
dc.identifier.citation	Plant Physiology and Biochemistry, 2014, 84 pp. 105 - 114	en_US
dc.identifier.issn	0981-9428	en_US
dc.identifier.uri	http://hdl.handle.net/10453/34021
dc.identifier.uri	http://hdl.handle.net/10453/33404
dc.description.abstract	© 2014 Elsevier Masson SAS. Small RNA profiling and assessing its dependence on changing environmental factors have expanded our understanding of the transcriptional and post-transcriptional regulation of plant stress responses. Insufficient data have been documented earlier to depict the profiling of small RNA classes in temperature-associated stress which has a wide implication for climate change biology. In the present study, we report a comparative assessment of the genome-wide profiling of small RNAs in Arabidopsis thaliana using two conditional responses, induced by high- and low-temperature. Genome-wide profiling of small RNAs revealed an abundance of 21nt small RNAs at low temperature, while high temperature showed an abundance of 21nt and 24nt small RNAs. The two temperature treatments altered the expression of a specific subset of mature miRNAs and displayed differential expression of a number of miRNA isoforms (isomiRs). Comparative analysis demonstrated that a large number of protein-coding genes can give rise to differentially expressed small RNAs following temperature shifts. Low temperature caused accumulation of small RNAs, corresponding to the sense strand of a number of cold-responsive genes. In contrast, high temperature stimulated the production of small RNAs of both polarities from genes encoding functionally diverse proteins.	en_US
dc.relation.ispartof	Plant Physiology and Biochemistry	en_US
dc.relation.isbasedon	10.1016/j.plaphy.2014.09.007	en_US
dc.subject.classification	Plant Biology & Botany	en_US
dc.subject.mesh	Arabidopsis	en_US
dc.subject.mesh	MicroRNAs	en_US
dc.subject.mesh	RNA, Plant	en_US
dc.subject.mesh	Temperature	en_US
dc.subject.mesh	Gene Expression Regulation, Plant	en_US
dc.subject.mesh	High-Throughput Nucleotide Sequencing	en_US
dc.title	Insight into small RNA abundance and expression in high- and low-temperature stress response using deep sequencing in Arabidopsis	en_US
dc.type	Journal Article
utslib.citation.volume	84	en_US
utslib.for	0503 Soil Sciences	en_US
utslib.for	0607 Plant Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.publication-status	Published	en_US
pubs.volume	84	en_US

Abstract:

© 2014 Elsevier Masson SAS. Small RNA profiling and assessing its dependence on changing environmental factors have expanded our understanding of the transcriptional and post-transcriptional regulation of plant stress responses. Insufficient data have been documented earlier to depict the profiling of small RNA classes in temperature-associated stress which has a wide implication for climate change biology. In the present study, we report a comparative assessment of the genome-wide profiling of small RNAs in Arabidopsis thaliana using two conditional responses, induced by high- and low-temperature. Genome-wide profiling of small RNAs revealed an abundance of 21nt small RNAs at low temperature, while high temperature showed an abundance of 21nt and 24nt small RNAs. The two temperature treatments altered the expression of a specific subset of mature miRNAs and displayed differential expression of a number of miRNA isoforms (isomiRs). Comparative analysis demonstrated that a large number of protein-coding genes can give rise to differentially expressed small RNAs following temperature shifts. Low temperature caused accumulation of small RNAs, corresponding to the sense strand of a number of cold-responsive genes. In contrast, high temperature stimulated the production of small RNAs of both polarities from genes encoding functionally diverse proteins.

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http://hdl.handle.net/10453/33404