Simultaneous determination of endocrine disrupting phenolic compounds and steroids in water by solid-phase extraction-gas chromatography-mass spectrometry

Liu, R; Zhou, JL; Wilding, A

Simultaneous determination of endocrine disrupting phenolic compounds and steroids in water by solid-phase extraction-gas chromatography-mass spectrometry

Liu, R Zhou, JL

Wilding, A

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Publication Type:: Journal Article
Citation:: Journal of Chromatography A, 2004, 1022 (1-2), pp. 179 - 189
Issue Date:: 2004-01-02

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Field	Value	Language
dc.contributor.author	Liu, R	en_US
dc.contributor.author	Zhou, JL https://orcid.org/0000-0003-1393-1608	en_US
dc.contributor.author	Wilding, A	en_US
dc.date.issued	2004-01-02	en_US
dc.identifier.citation	Journal of Chromatography A, 2004, 1022 (1-2), pp. 179 - 189	en_US
dc.identifier.issn	0021-9673	en_US
dc.identifier.uri	http://hdl.handle.net/10453/33729
dc.description.abstract	A solid-phase extraction (SPE)-gas chromatography (GC)-mass spectrometry (MS) analytical method for the simultaneous separation and determination of endocrine disrupting chemicals (EDCs) from water samples is described in detail. Important and contrasting EDCs including estrone, 17β-estradiol, 17α-ethynylestradiol, 16α-hydroxyestrone, 4-nonylphenol, bisphenol A and 4-tert-octylphenol were selected as the target compounds. The SPE technique, followed by the derivatisation with bis (trimethylsilyl) trifluoroacetamide was used for the extraction recoveries of target compounds from water samples. A number of parameters that may affect the recovery of EDCs, such as the type of SPE cartridges, eluents, as well as water properties including pH value, and concentration of salts and humic substances were investigated. It is shown that the Oasis cartridges produced the best recoveries of target EDCs while ethyl acetate was efficient in eluting EDCs from SPE cartridges. The recovery of some EDCs was enhanced by the addition of salt, but reduced by the increase in pH value and humic acid concentration. The optimised method was further verified by performing spiking experiments in natural river water and seawater matrices, with good recovery and reproducibility for all the selected compounds. The established method was successfully applied to environmental water samples from East and West Sussex, UK, for the determination of the target EDCs. © 2003 Elsevier B.V. All rights reserved.	en_US
dc.relation.ispartof	Journal of Chromatography A	en_US
dc.relation.isbasedon	10.1016/j.chroma.2003.09.035	en_US
dc.subject.classification	Analytical Chemistry	en_US
dc.subject.mesh	Endocrine Glands	en_US
dc.subject.mesh	Phenols	en_US
dc.subject.mesh	Steroids	en_US
dc.subject.mesh	Environmental Pollutants	en_US
dc.subject.mesh	Calibration	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Reproducibility of Results	en_US
dc.subject.mesh	Gas Chromatography-Mass Spectrometry	en_US
dc.title	Simultaneous determination of endocrine disrupting phenolic compounds and steroids in water by solid-phase extraction-gas chromatography-mass spectrometry	en_US
dc.type	Journal Article
utslib.citation.volume	1-2	en_US
utslib.citation.volume	1022	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	09 Engineering	en_US
utslib.for	10 Technology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access
pubs.issue	1-2	en_US
pubs.publication-status	Published	en_US
pubs.volume	1022	en_US

Abstract:

A solid-phase extraction (SPE)-gas chromatography (GC)-mass spectrometry (MS) analytical method for the simultaneous separation and determination of endocrine disrupting chemicals (EDCs) from water samples is described in detail. Important and contrasting EDCs including estrone, 17β-estradiol, 17α-ethynylestradiol, 16α-hydroxyestrone, 4-nonylphenol, bisphenol A and 4-tert-octylphenol were selected as the target compounds. The SPE technique, followed by the derivatisation with bis (trimethylsilyl) trifluoroacetamide was used for the extraction recoveries of target compounds from water samples. A number of parameters that may affect the recovery of EDCs, such as the type of SPE cartridges, eluents, as well as water properties including pH value, and concentration of salts and humic substances were investigated. It is shown that the Oasis cartridges produced the best recoveries of target EDCs while ethyl acetate was efficient in eluting EDCs from SPE cartridges. The recovery of some EDCs was enhanced by the addition of salt, but reduced by the increase in pH value and humic acid concentration. The optimised method was further verified by performing spiking experiments in natural river water and seawater matrices, with good recovery and reproducibility for all the selected compounds. The established method was successfully applied to environmental water samples from East and West Sussex, UK, for the determination of the target EDCs. © 2003 Elsevier B.V. All rights reserved.

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