Validation of cross-flow ultrafiltration for sampling of colloidal particles from aquatic systems

Wilding, A; Liu, R; Zhou, JL

Validation of cross-flow ultrafiltration for sampling of colloidal particles from aquatic systems

Wilding, A Liu, R Zhou, JL

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Publication Type:: Journal Article
Citation:: Journal of Colloid and Interface Science, 2004, 280 (1), pp. 102 - 112
Issue Date:: 2004-12-01

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Field	Value	Language
dc.contributor.author	Wilding, A	en_US
dc.contributor.author	Liu, R	en_US
dc.contributor.author	Zhou, JL https://orcid.org/0000-0003-1393-1608	en_US
dc.date.available	2004-07-13	en_US
dc.date.issued	2004-12-01	en_US
dc.identifier.citation	Journal of Colloid and Interface Science, 2004, 280 (1), pp. 102 - 112	en_US
dc.identifier.issn	0021-9797	en_US
dc.identifier.uri	http://hdl.handle.net/10453/33731
dc.description.abstract	The use of cross-flow ultrafiltration (CFF) for the isolation of colloids from the bulk aquatic matrices has been increasing over the last decade; however, a thorough validation of each CFF system is needed before its application to natural samples. Laboratory experiments have been conducted to validate a 1-kDa Millipore Pellicon 2 cartridge type CFF system, using a range of molecular probes spiked in natural waters. Using the separation data of these probes, key validation parameters have been calculated, including retention coefficient (RC), concentration factor (cf), colloid concentration, recovery, and mass balance. The permeation behaviour of these molecules has also been studied using a simple permeation model. Results show that good retention (>80%) of high molecular weight (HMW, >1 kDa) molecules and low retention (<20%) of low molecular weight (LMW, <1 kDa) molecules can be achieved at high cf values or over long timescales. In addition, permeation modelling and the calculation of colloid concentration demonstrate the same findings, again recommending high cf values or long timescales for ultrafiltration to minimise the retention of LMW molecules. The retention characteristics of 3-kDa dextran change slightly with salinity. Mass balance calculations show good recoveries for all of the molecules tested. The apparent membrane molecular cut-off of the CFF system has been estimated to be between 2.1 and 2.5 kDa, which is slightly greater than the manufacturer's nominal MW cut-off of 1 kDa. © 2004 Elsevier Inc. All rights reserved.	en_US
dc.relation.ispartof	Journal of Colloid and Interface Science	en_US
dc.relation.isbasedon	10.1016/j.jcis.2004.07.002	en_US
dc.subject.classification	Chemical Physics	en_US
dc.title	Validation of cross-flow ultrafiltration for sampling of colloidal particles from aquatic systems	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	280	en_US
utslib.for	02 Physical Sciences	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	09 Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Engineering and Information Technology/School of Civil and Environmental Engineering
pubs.organisational-group	/University of Technology Sydney/Strength - CTWW - Centre for Technology in Water and Wastewater Treatment
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	280	en_US

Abstract:

The use of cross-flow ultrafiltration (CFF) for the isolation of colloids from the bulk aquatic matrices has been increasing over the last decade; however, a thorough validation of each CFF system is needed before its application to natural samples. Laboratory experiments have been conducted to validate a 1-kDa Millipore Pellicon 2 cartridge type CFF system, using a range of molecular probes spiked in natural waters. Using the separation data of these probes, key validation parameters have been calculated, including retention coefficient (RC), concentration factor (cf), colloid concentration, recovery, and mass balance. The permeation behaviour of these molecules has also been studied using a simple permeation model. Results show that good retention (>80%) of high molecular weight (HMW, >1 kDa) molecules and low retention (<20%) of low molecular weight (LMW, <1 kDa) molecules can be achieved at high cf values or over long timescales. In addition, permeation modelling and the calculation of colloid concentration demonstrate the same findings, again recommending high cf values or long timescales for ultrafiltration to minimise the retention of LMW molecules. The retention characteristics of 3-kDa dextran change slightly with salinity. Mass balance calculations show good recoveries for all of the molecules tested. The apparent membrane molecular cut-off of the CFF system has been estimated to be between 2.1 and 2.5 kDa, which is slightly greater than the manufacturer's nominal MW cut-off of 1 kDa. © 2004 Elsevier Inc. All rights reserved.

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http://hdl.handle.net/10453/33731