Selective extraction of bacterial DNA from the surfaces of macroalgaeδ

Burke, C; Kjelleberg, S; Thomas, T

Selective extraction of bacterial DNA from the surfaces of macroalgaeδ

Burke, C

Kjelleberg, S Thomas, T

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Publication Type:: Journal Article
Citation:: Applied and Environmental Microbiology, 2009, 75 (1), pp. 252 - 256
Issue Date:: 2009-01-01

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Field	Value	Language
dc.contributor.author	Burke, C https://orcid.org/0000-0002-8917-0019	en_US
dc.contributor.author	Kjelleberg, S	en_US
dc.contributor.author	Thomas, T	en_US
dc.date.issued	2009-01-01	en_US
dc.identifier.citation	Applied and Environmental Microbiology, 2009, 75 (1), pp. 252 - 256	en_US
dc.identifier.issn	0099-2240	en_US
dc.identifier.uri	http://hdl.handle.net/10453/34561
dc.description.abstract	A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing. © 2009, American Society for Microbiology. All Rights Reserved.	en_US
dc.relation.ispartof	Applied and Environmental Microbiology	en_US
dc.relation.isbasedon	10.1128/AEM.01630-08	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Ulva	en_US
dc.subject.mesh	DNA, Bacterial	en_US
dc.subject.mesh	Sensitivity and Specificity	en_US
dc.subject.mesh	Molecular Biology	en_US
dc.subject.mesh	Rhodophyta	en_US
dc.title	Selective extraction of bacterial DNA from the surfaces of macroalgaeδ	en_US
dc.type	Journal Article
utslib.citation.volume	1	en_US
utslib.citation.volume	75	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	1	en_US
pubs.publication-status	Published	en_US
pubs.volume	75	en_US

Abstract:

A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing. © 2009, American Society for Microbiology. All Rights Reserved.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/34561