Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy

Sackett, O; Armand, L; Beardall, J; Hill, R; Doblin, M; Connelly, C; Howes, J; Stuart, B; Ralph, P; Heraud, P

Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy

Sackett, O Armand, L Beardall, J Hill, R Doblin, M

Connelly, C Howes, J Stuart, B

Ralph, P

Heraud, P

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Publication Type:: Journal Article
Citation:: Biogeosciences, 2014, 11 (20), pp. 5795 - 5808
Issue Date:: 2014-10-20

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Field	Value	Language
dc.contributor.author	Sackett, O	en_US
dc.contributor.author	Armand, L	en_US
dc.contributor.author	Beardall, J	en_US
dc.contributor.author	Hill, R	en_US
dc.contributor.author	Doblin, M https://orcid.org/0000-0001-8750-3433	en_US
dc.contributor.author	Connelly, C	en_US
dc.contributor.author	Howes, J	en_US
dc.contributor.author	Stuart, B https://orcid.org/0000-0001-9540-4435	en_US
dc.contributor.author	Ralph, P https://orcid.org/0000-0002-3103-7346	en_US
dc.contributor.author	Heraud, P	en_US
dc.date.issued	2014-10-20	en_US
dc.identifier.citation	Biogeosciences, 2014, 11 (20), pp. 5795 - 5808	en_US
dc.identifier.issn	1726-4170	en_US
dc.identifier.uri	http://hdl.handle.net/10453/35877
dc.description.abstract	© 2014 Author(s). Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of elements (e.g. C, Si and Fe) from surface waters as a result of Fe addition. Less is known about the effects of Fe enrichment on the phenotypes of diatoms, such as associated changes in nutritional value-furthermore, data on taxon-specific responses are almost non-existent. Enhanced supply of nutrient-rich waters along the coast of the subantarctic Kerguelen Island provide a valuable opportunity to examine the responses of phytoplankton to natural Fe enrichment. Here we demonstrate the use of synchrotron radiation Fourier Transform Infrared (SR-FTIR) microspectroscopy to analyse changes in the macromolecular composition of diatoms collected along the coast and plateau of Kerguelen Island, Southern Ocean. SR-FTIR microspectroscopy enabled the analysis of individual diatom cells from mixed communities of field-collected samples, thereby providing insight into in situ taxon-specific responses in relation to changes in Fe availability. Phenotypic responses were taxon-specific in terms of intraspecific variability and changes in proteins, amino acids, phosphorylated molecules, silicate/silicic acid and carbohydrates. In contrast to some previous studies, silicate/silicic acid levels increased under Fe enrichment, in conjunction with increases in carbohydrate stores. The highly abundant taxon Fragilariopsis kerguelensis displayed a higher level of phenotypic plasticity than Pseudo-nitzschia spp., while analysis of the data pooled across all measured taxa showed different patterns in macromolecular composition compared to those for individual taxon. This study demonstrates that taxon-specific responses to Fe enrichment may not always be accurately reflected by bulk community measurements, highlighting the need for further research into taxon-specific phenotypic responses of phytoplankton to environmental change.	en_US
dc.relation.ispartof	Biogeosciences	en_US
dc.relation.isbasedon	10.5194/bg-11-5795-2014	en_US
dc.subject.classification	Meteorology & Atmospheric Sciences	en_US
dc.title	Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy	en_US
dc.type	Journal Article
utslib.citation.volume	20	en_US
utslib.citation.volume	11	en_US
utslib.for	0602 Ecology	en_US
utslib.for	0499 Other Earth Sciences	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	open_access
pubs.issue	20	en_US
pubs.publication-status	Published	en_US
pubs.volume	11	en_US

Abstract:

© 2014 Author(s). Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of elements (e.g. C, Si and Fe) from surface waters as a result of Fe addition. Less is known about the effects of Fe enrichment on the phenotypes of diatoms, such as associated changes in nutritional value-furthermore, data on taxon-specific responses are almost non-existent. Enhanced supply of nutrient-rich waters along the coast of the subantarctic Kerguelen Island provide a valuable opportunity to examine the responses of phytoplankton to natural Fe enrichment. Here we demonstrate the use of synchrotron radiation Fourier Transform Infrared (SR-FTIR) microspectroscopy to analyse changes in the macromolecular composition of diatoms collected along the coast and plateau of Kerguelen Island, Southern Ocean. SR-FTIR microspectroscopy enabled the analysis of individual diatom cells from mixed communities of field-collected samples, thereby providing insight into in situ taxon-specific responses in relation to changes in Fe availability. Phenotypic responses were taxon-specific in terms of intraspecific variability and changes in proteins, amino acids, phosphorylated molecules, silicate/silicic acid and carbohydrates. In contrast to some previous studies, silicate/silicic acid levels increased under Fe enrichment, in conjunction with increases in carbohydrate stores. The highly abundant taxon Fragilariopsis kerguelensis displayed a higher level of phenotypic plasticity than Pseudo-nitzschia spp., while analysis of the data pooled across all measured taxa showed different patterns in macromolecular composition compared to those for individual taxon. This study demonstrates that taxon-specific responses to Fe enrichment may not always be accurately reflected by bulk community measurements, highlighting the need for further research into taxon-specific phenotypic responses of phytoplankton to environmental change.

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http://hdl.handle.net/10453/35877