Molecular Interactions of the Min Protein System Reproduce Spatiotemporal Patterning in Growing and Dividing Escherichia coli Cells

Walsh, JC; Angstmann, CN; Duggin, IG; Curmi, PMG

Molecular Interactions of the Min Protein System Reproduce Spatiotemporal Patterning in Growing and Dividing Escherichia coli Cells

Walsh, JC Angstmann, CN Duggin, IG

Curmi, PMG

Permalink

Publication Type:: Journal Article
Citation:: PLoS ONE, 2015, 10 (5)
Issue Date:: 2015-05-01

Open Access

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is open access.

Adobe PDF

Download Published VersionAdobe PDF (2.35 MB)

View on publisher's site

View statistics

Full metadata record

Field	Value	Language
dc.contributor.author	Walsh, JC	en_US
dc.contributor.author	Angstmann, CN	en_US
dc.contributor.author	Duggin, IG https://orcid.org/0000-0003-4868-7350	en_US
dc.contributor.author	Curmi, PMG	en_US
dc.date.available	2015-04-22	en_US
dc.date.issued	2015-05-01	en_US
dc.identifier.citation	PLoS ONE, 2015, 10 (5)	en_US
dc.identifier.uri	http://hdl.handle.net/10453/36037
dc.description.abstract	© 2015 Walsh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Oscillations of the Min protein system are involved in the correct midcell placement of the divisome during Escherichia coli cell division. Based on molecular interactions of the Min system, we formulated a mathematical model that reproduces Min patterning during cell growth and division. Specifically, the increase in the residence time of MinD attached to the membrane as its own concentration increases, is accounted for by dimerisation of membrane- bound MinD and its interaction with MinE. Simulation of this system generates unparalleled correlation between the waveshape of experimental and theoretical MinD distributions, suggesting that the dominant interactions of the physical system have been successfully incorporated into the model. For cells where MinD is fully-labelled with GFP, the model reproduces the stationary localization of MinD-GFP for short cells, followed by oscillations from pole to pole in larger cells, and the transition to the symmetric distribution during cell filamentation. Cells containing a secondary, GFP-labelled MinD display a contrasting pattern. The model is able to account for these differences, including temporary midcell localization just prior to division, by increasing the rate constant controlling MinD ATPase and heterotetramer dissociation. For both experimental conditions, the model can explain how cell division results in an equal distribution of MinD and MinE in the two daughter cells, and accounts for the temperature dependence of the period of Min oscillations. Thus, we show that while other interactions may be present, they are not needed to reproduce the main characteristics of the Min system in vivo.	en_US
dc.relation.ispartof	PLoS ONE	en_US
dc.relation.isbasedon	10.1371/journal.pone.0128148	en_US
dc.subject.classification	General Science & Technology	en_US
dc.subject.mesh	Cell Membrane	en_US
dc.subject.mesh	Escherichia coli	en_US
dc.subject.mesh	Escherichia coli Proteins	en_US
dc.subject.mesh	Cell Cycle Proteins	en_US
dc.subject.mesh	Green Fluorescent Proteins	en_US
dc.subject.mesh	Membrane Proteins	en_US
dc.subject.mesh	Adenosine Triphosphatases	en_US
dc.subject.mesh	Biological Phenomena	en_US
dc.subject.mesh	Cell Division	en_US
dc.subject.mesh	Models, Theoretical	en_US
dc.title	Molecular Interactions of the Min Protein System Reproduce Spatiotemporal Patterning in Growing and Dividing Escherichia coli Cells	en_US
dc.type	Journal Article
utslib.citation.volume	5	en_US
utslib.citation.volume	10	en_US
utslib.for	0605 Microbiology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access
pubs.issue	5	en_US
pubs.publication-status	Published	en_US
pubs.volume	10	en_US

Abstract:

© 2015 Walsh et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Oscillations of the Min protein system are involved in the correct midcell placement of the divisome during Escherichia coli cell division. Based on molecular interactions of the Min system, we formulated a mathematical model that reproduces Min patterning during cell growth and division. Specifically, the increase in the residence time of MinD attached to the membrane as its own concentration increases, is accounted for by dimerisation of membrane- bound MinD and its interaction with MinE. Simulation of this system generates unparalleled correlation between the waveshape of experimental and theoretical MinD distributions, suggesting that the dominant interactions of the physical system have been successfully incorporated into the model. For cells where MinD is fully-labelled with GFP, the model reproduces the stationary localization of MinD-GFP for short cells, followed by oscillations from pole to pole in larger cells, and the transition to the symmetric distribution during cell filamentation. Cells containing a secondary, GFP-labelled MinD display a contrasting pattern. The model is able to account for these differences, including temporary midcell localization just prior to division, by increasing the rate constant controlling MinD ATPase and heterotetramer dissociation. For both experimental conditions, the model can explain how cell division results in an equal distribution of MinD and MinE in the two daughter cells, and accounts for the temperature dependence of the period of Min oscillations. Thus, we show that while other interactions may be present, they are not needed to reproduce the main characteristics of the Min system in vivo.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/36037