Practical implementation, characterization and applications of a multi-colour time-gated luminescence microscope

Zhang, L; Zheng, X; Deng, W; Lu, Y; Lechevallier, S; Ye, Z; Goldys, EM; Dawes, JM; Piper, JA; Yuan, J; Verelst, M; Jin, D

Practical implementation, characterization and applications of a multi-colour time-gated luminescence microscope

Zhang, L Zheng, X Deng, W Lu, Y Lechevallier, S Ye, Z Goldys, EM Dawes, JM Piper, JA Yuan, J Verelst, M Jin, D

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Publication Type:: Journal Article
Citation:: Scientific Reports, 2014, 4
Issue Date:: 2014-10-13

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Field	Value	Language
dc.contributor.author	Zhang, L	en_US
dc.contributor.author	Zheng, X	en_US
dc.contributor.author	Deng, W	en_US
dc.contributor.author	Lu, Y	en_US
dc.contributor.author	Lechevallier, S	en_US
dc.contributor.author	Ye, Z	en_US
dc.contributor.author	Goldys, EM	en_US
dc.contributor.author	Dawes, JM	en_US
dc.contributor.author	Piper, JA	en_US
dc.contributor.author	Yuan, J	en_US
dc.contributor.author	Verelst, M	en_US
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666	en_US
dc.date.available	2014-09-17	en_US
dc.date.issued	2014-10-13	en_US
dc.identifier.citation	Scientific Reports, 2014, 4	en_US
dc.identifier.uri	http://hdl.handle.net/10453/36929
dc.description.abstract	Time-gated luminescence microscopy using long-lifetime molecular probes can effectively eliminate autofluorescence to enable high contrast imaging. Here we investigate a new strategy of time-gated imaging for simultaneous visualisation of multiple species of microorganisms stained with long-lived complexes under low-background conditions. This is realized by imaging two pathogenic organisms (Giardia lamblia stained with a red europium probe and Cryptosporidium parvum with a green terbium probe) at UV wavelengths (320-400 nm) through synchronization of a flash lamp with high repetition rate (1 kHz) to a robust time-gating detection unit. This approach provides four times enhancement in signal-to-background ratio over non-time-gated imaging, while the average signal intensity also increases six-fold compared with that under UV LED excitation. The high sensitivity is further confirmed by imaging the single europium-doped Y<inf>2</inf>O<inf>2</inf>S nanocrystals (150 nm). We report technical details regarding the time-gating detection unit and demonstrate its compatibility with commercial epi-fluorescence microscopes, providing a valuable and convenient addition to standard laboratory equipment.	en_US
dc.relation	http://purl.org/au-research/grants/arc/FT130100517
dc.relation.ispartof	Scientific Reports	en_US
dc.relation.isbasedon	10.1038/srep06597	en_US
dc.subject.mesh	Cryptosporidium parvum	en_US
dc.subject.mesh	Europium	en_US
dc.subject.mesh	Microscopy	en_US
dc.subject.mesh	Microscopy, Fluorescence	en_US
dc.subject.mesh	Luminescent Measurements	en_US
dc.subject.mesh	Molecular Imaging	en_US
dc.title	Practical implementation, characterization and applications of a multi-colour time-gated luminescence microscope	en_US
dc.type	Journal Article
utslib.citation.volume	4	en_US
utslib.for	1007 Nanotechnology	en_US
utslib.for	0903 Biomedical Engineering	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US
pubs.volume	4	en_US

Abstract:

Time-gated luminescence microscopy using long-lifetime molecular probes can effectively eliminate autofluorescence to enable high contrast imaging. Here we investigate a new strategy of time-gated imaging for simultaneous visualisation of multiple species of microorganisms stained with long-lived complexes under low-background conditions. This is realized by imaging two pathogenic organisms (Giardia lamblia stained with a red europium probe and Cryptosporidium parvum with a green terbium probe) at UV wavelengths (320-400 nm) through synchronization of a flash lamp with high repetition rate (1 kHz) to a robust time-gating detection unit. This approach provides four times enhancement in signal-to-background ratio over non-time-gated imaging, while the average signal intensity also increases six-fold compared with that under UV LED excitation. The high sensitivity is further confirmed by imaging the single europium-doped Y2O2S nanocrystals (150 nm). We report technical details regarding the time-gating detection unit and demonstrate its compatibility with commercial epi-fluorescence microscopes, providing a valuable and convenient addition to standard laboratory equipment.

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http://hdl.handle.net/10453/36929