An in vitro study of the effects of exposure to a GSM signal in two human cell lines: Monocytic U937 and neuroblastoma SK-N-SH

Gurisik, E; Warton, K; Martin, DK; Valenzuela, SM

An in vitro study of the effects of exposure to a GSM signal in two human cell lines: Monocytic U937 and neuroblastoma SK-N-SH

Gurisik, E Warton, K Martin, DK

Valenzuela, SM

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Publication Type:: Journal Article
Citation:: Cell Biology International, 2006, 30 (10), pp. 793 - 799
Issue Date:: 2006-10-01

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Field	Value	Language
dc.contributor.author	Gurisik, E	en_US
dc.contributor.author	Warton, K	en_US
dc.contributor.author	Martin, DK https://orcid.org/0000-0001-5913-2372	en_US
dc.contributor.author	Valenzuela, SM https://orcid.org/0000-0001-5934-6047	en_US
dc.date.available	2006-06-23	en_US
dc.date.issued	2006-10-01	en_US
dc.identifier.citation	Cell Biology International, 2006, 30 (10), pp. 793 - 799	en_US
dc.identifier.issn	1065-6995	en_US
dc.identifier.uri	http://hdl.handle.net/10453/3714
dc.description.abstract	The use of mobile phones is increasing, which also increases the population's exposure to global system of mobile communications (GSM) signals. Questions of safety and possible biological effects are of concern and to date, remain largely unanswered. In order to examine possible biological effects of a GSM-like signal at a cellular level, we exposed two human cell lines (one of neuronal (SK-N-SH) and the other of monocytoid (U937) origin) to a 900 MHz RF signal, pulsed at 217 Hz, producing a specific absorption rate (SAR) of 0.2 W/kg. Putative effects were assessed by comparing radiofrequency-exposed cells to sham-exposed cells using a variety of assay techniques. For the cell line SK-N-SH, effects were specifically assessed by gene microarray, followed by real-time PCR of the genes of interest, Western blot analysis was used to measure heat shock protein levels, and flow cytometry to measure cell cycle distributions and apoptosis. Effects of radiofrequency on the cell line U937 were assessed by cell viability and cell cycle analysis. From our study of these two cell lines, we found no significant difference between sham-exposed versus radiofrequency-exposed cells in any of the assays or conditions examined. © 2006 International Federation for Cell Biology.	en_US
dc.relation.ispartof	Cell Biology International	en_US
dc.relation.isbasedon	10.1016/j.cellbi.2006.06.001	en_US
dc.subject.classification	Biochemistry & Molecular Biology	en_US
dc.subject.mesh	Cell Line	en_US
dc.subject.mesh	U937 Cells	en_US
dc.subject.mesh	Tumor Cells, Cultured	en_US
dc.subject.mesh	Humans	en_US
dc.subject.mesh	Heat-Shock Proteins	en_US
dc.subject.mesh	Cell Cycle	en_US
dc.subject.mesh	Apoptosis	en_US
dc.subject.mesh	Cell Survival	en_US
dc.subject.mesh	Gene Expression	en_US
dc.subject.mesh	Electromagnetic Fields	en_US
dc.subject.mesh	Radio Waves	en_US
dc.subject.mesh	Cellular Phone	en_US
dc.subject.mesh	Cell Physiological Phenomena	en_US
dc.subject.mesh	Cell Phone	en_US
dc.title	An in vitro study of the effects of exposure to a GSM signal in two human cell lines: Monocytic U937 and neuroblastoma SK-N-SH	en_US
dc.type	Journal Article
utslib.citation.volume	10	en_US
utslib.citation.volume	30	en_US
utslib.for	0601 Biochemistry and Cell Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Medical and Molecular Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
utslib.copyright.status	closed_access
pubs.issue	10	en_US
pubs.publication-status	Published	en_US
pubs.volume	30	en_US

Abstract:

The use of mobile phones is increasing, which also increases the population's exposure to global system of mobile communications (GSM) signals. Questions of safety and possible biological effects are of concern and to date, remain largely unanswered. In order to examine possible biological effects of a GSM-like signal at a cellular level, we exposed two human cell lines (one of neuronal (SK-N-SH) and the other of monocytoid (U937) origin) to a 900 MHz RF signal, pulsed at 217 Hz, producing a specific absorption rate (SAR) of 0.2 W/kg. Putative effects were assessed by comparing radiofrequency-exposed cells to sham-exposed cells using a variety of assay techniques. For the cell line SK-N-SH, effects were specifically assessed by gene microarray, followed by real-time PCR of the genes of interest, Western blot analysis was used to measure heat shock protein levels, and flow cytometry to measure cell cycle distributions and apoptosis. Effects of radiofrequency on the cell line U937 were assessed by cell viability and cell cycle analysis. From our study of these two cell lines, we found no significant difference between sham-exposed versus radiofrequency-exposed cells in any of the assays or conditions examined. © 2006 International Federation for Cell Biology.

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http://hdl.handle.net/10453/3714